High-throughput sequencing identification of genes involved with Varroa destructor resistance in the eastern honeybee, Apis cerana

Ji, Ting; Yin, Ling; Liu, Z; Shen, Fang; Shen, Jie

doi:10.4238/2014.october.31.24

Cited by 7 publications

(6 citation statements)

References 37 publications

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“…Indeed, transcripts of the calcium-transporting ATPase sarcoplasmic/endoplasmic reticulum (SERCA) and of the sarcalumenin were upregulated in CYp-infected insects. The former is a pump involved in translocation of cytosolic calcium into the sarcoplasmic reticulum to allow relaxation of muscle fibers (16); the latter is a calcium-binding protein involved in fine regulation of cellular calcium storage (52). Moreover, transcripts of the main proteins involved in contraction and cytoskeletal motion, namely, tropomyosin, troponin, myosin, actin, and dynein (16), were all upregulated upon CYp infection.…”

Section: Discussionmentioning

confidence: 99%

Two Phytoplasmas Elicit Different Responses in the Insect Vector Euscelidius variegatus Kirschbaum

et al. 2018

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Phytoplasmas are plant-pathogenic bacteria transmitted by hemipteran insects. The leafhopper is a natural vector of chrysanthemum yellows phytoplasma (CYp) and a laboratory vector of flavescence dorée phytoplasma (FDp). The two phytoplasmas induce different effects on this species: CYp slightly improves whereas FDp negatively affects insect fitness. To investigate the molecular bases of these different responses, transcriptome sequencing (RNA-seq) analysis of infected with either CYp or FDp was performed. The sequencing provided the first transcriptome assembly for a phytoplasma vector and a starting point for further analyses on differentially regulated genes, mainly related to immune system and energy metabolism. Insect phenoloxidase activity, immunocompetence, and body pigmentation were measured to investigate the immune response, while respiration and movement rates were quantified to confirm the effects on energy metabolism. The activation of the insect immune response upon infection with FDp, which is not naturally transmitted by, confirmed that this bacterium is mostly perceived as a potential pathogen. Conversely, the acquisition of CYp, which is naturally transmitted by , seems to increase the insect fitness by inducing a prompt response to stress. This long-term relationship is likely to improve survival and dispersal of the infected insect, thus enhancing the opportunity of phytoplasma transmission.

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Section: Discussionmentioning

confidence: 99%

Two Phytoplasmas Elicit Different Responses in the Insect Vector Euscelidius variegatus Kirschbaum

et al. 2018

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“…In Asia, and in particular in China, beekeepers use the indigenous honey bee A. cerana in addition to non-native A. mellifera . The Asian honey bee has coevolved with Varroa mites ( Varroa jacobsoni and V. destructor ) and possesses behavioural 27–30 and physiological resistance mechanisms against these mites 31 , which may protect them from the current global DWV epidemic 26 . At the same time, East Asia is the epicentre of disease emergence for bees: both the Varroa mite and the gut parasite Nosema ceranae have jumped from the Asian honey bee A. cerana to the European honey bee A. mellifera in East Asia in the last century and have spread globally.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and population genetics of the emerging honey bee pathogen DWV in Chinese apiculture

Diao

Yang

Zhao³

et al. 2019

Sci Rep

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Honey bees are agriculturally important, both as pollinators and by providing products such as honey. The sustainability of beekeeping is at risk through factors of global change such as habitat loss, as well as through the spread of infectious diseases. In China and other parts of Asia, beekeepers rely both on native Apis cerana and non-native Apis mellifera, putting bee populations at particular risk of disease emergence from multi-host pathogens. Indeed, two important honey bee parasites have emerged from East Asian honey bees, the mite Varroa destructor and the microsporidian Nosema ceranae. As V. destructor vectors viral bee diseases, we investigated whether another key bee pathogen, Deformed Wing Virus (DWV), may also have originated in East Asian honey bee populations. We use a large-scale survey of apiaries across china to investigate the prevalence and seasonality of DWV in managed A. mellifera and A. cerana colonies, showing that DWV-A prevalence was higher in A. mellifera, with a seasonal spike in prevalence in autumn and winter. Using phylogenetic and population genetic approaches, we show that while China and East Asian DWV isolates show comparatively high levels of genetic diversity, these bee populations are not a source for the current global DWV epidemic.

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“…The honey bee transcriptome in which we found the first Apis densovirus was erroneously classified as Apis mellifera carnica transcriptome (SRR922440, 52.177 con-tigs) and is still available under such name. It was produced from the whole heads of the workers by the researchers from the yangzhou University, Jiangsan Province, China (Ji et al, 2014). The analysis of the SRR922440 transcriptome showed that 86 % of contigs have strong signals to the Apis cerana and only 1.2 % to the Apis mellifera.…”

Section: Information About the Origin Of The Eastern Honey Bee Transcmentioning

confidence: 99%

“…This analysis demonstrated that the SRR922440 transcriptome originates from the Apis cerana, since numerous sequences show 99 % identity with the mitochondrial genome of A. cerana. The authors of this transcriptome (Ji et al, 2014) provided some information about the origin of the A. cerana colonies: M and C colonies were unrelated local strains in the same A. cerana population and were bred in the Guandong Entomological Institute, Guangzhou (also known in the West as the Canton), southern China. All Apis densovirus transcripts were obtained from the C colony that was Varroa resistant.…”

Section: Information About the Origin Of The Eastern Honey Bee Transcmentioning

confidence: 99%

“…All Apis densovirus transcripts were obtained from the C colony that was Varroa resistant. No information about the health status of the bees was provided in the original reference (Ji et al, 2014). We checked if these bees were infected with some RNA viruses, discovering only a fulllength Sacbrood virus in this transcriptome (in the sequence GALO01042235).…”

Section: Information About the Origin Of The Eastern Honey Bee Transcmentioning

confidence: 99%

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Discovery and molecular characterisation of the first ambidensovirus in honey bees

Rutar

Kordiš

2020

AAS

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Honey bees play a critical role in global food production as pollinators of numerous crops. Several stressors cause declines in populations of managed and wild bee species, such as habitat degradation, pesticide exposure and pathogens. Viruses act as key stressors and can infect a wide range of species. The majority of honey bee-infecting viruses are RNA viruses of the Picornavirales order. Although some ssDNA viruses are common in insects, such as densoviruses, they have not yet been found in honey bees. Densoviruses were however found in bumblebees and ants. Here, we show that densoviruses are indeed present in the transcriptome of the eastern honey bee (<em>Apis cerana</em>) from southern China. On the basis of non-structural and structural transcripts, we inferred the genome structure of the Apis densovirus. Phylogenetic analysis has shown that this novel Apis densovirus belongs to the <em>Scindoambidensovirus</em> genus in the Densovirinae subfamily. Apis densovirus possesses ambisense genome organisation and encodes three non-structural proteins and a split VP (capsid) protein. The availability of a nearly complete Apis densovirus genome may enable the analysis of its potential pathogenic impact on honey bees. Our findings can thus guide further research into the densoviruses in honey bees and bumblebees.

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High-throughput sequencing identification of genes involved with Varroa destructor resistance in the eastern honeybee, Apis cerana

Cited by 7 publications

References 37 publications

Two Phytoplasmas Elicit Different Responses in the Insect Vector Euscelidius variegatus Kirschbaum

Two Phytoplasmas Elicit Different Responses in the Insect Vector Euscelidius variegatus Kirschbaum

Prevalence and population genetics of the emerging honey bee pathogen DWV in Chinese apiculture

Discovery and molecular characterisation of the first ambidensovirus in honey bees

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