High-Throughput Sequencing Discloses the Cucumber Mosaic Virus (CMV) Diversity in Slovakia and Reveals New Hosts of CMV from the Papaveraceae Family

Mrkvová, Michaela; Hančinský, Richard; Predajňa, Lukáš; Alaxin, Peter; Achs, Adam; Tomašechová, Jana; Šoltýs, Katarína; Mihálik, Daniel; Olmos, Antonio; Ruiz-García, Ana Belén; Glasa, Miroslav

doi:10.3390/plants11131665

Cited by 6 publications

(4 citation statements)

References 51 publications

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“…On the contrary, only a single BYMV infection was found in the PS2 sample (Table 1). Currently, it is largely proven through virome analyses that mixed infections of plants are frequently found in nature, complicating the elucidation of disease etiology [24,29,30]. The presence of multiple viruses in both DAT and FA40 plants made it difficult to decipher the role of any single virus in causing the observed symptoms (Figure 1).…”

Section: Resultsmentioning

confidence: 99%

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Molecular Characteristics and Biological Properties of Bean Yellow Mosaic Virus Isolates from Slovakia

Mrkvová,

Kemenczeiová,

Achs

et al. 2024

Horticulturae

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Analysis of the viromes of three symptomatic Fabaceae plants, i.e., red clover (Trifolium pratense L.), pea (Pisum sativum L.), and common bean (Phaseolus vulgaris L.), using high-throughput sequencing revealed complex infections and enabled the acquisition of complete genomes of a potyvirus, bean yellow mosaic virus (BYMV). Based on phylogenetic analysis, the Slovak BYMV isolates belong to two distinct molecular groups, i.e., VI (isolate FA40) and XI (isolates DAT, PS2). Five commercial pea genotypes (Alderman, Ambrosia, Gloriosa, Herkules, Senator) were successfully infected with the BYMV-PS2 inoculum and displayed similar systemic chlorotic mottling symptoms. Relative comparison of optical density values using semi-quantitative DAS-ELISA revealed significant differences among virus titers in one of the infected pea genotypes (Ambrosia) when upper fully developed leaves were tested. Immunoblot analysis of systemically infected Alderman plants showed rather uneven virus accumulation in different plant parts. The lowest virus accumulation was repeatedly detected in the roots, while the highest was in the upper part of the plant stem.

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Section: Resultsmentioning

confidence: 99%

“…HTS analysis of the Fabaceae samples was performed, as described previously [24]. Briefly, total RNAs were extracted from the leaves of original host plants using a Spectrum TM Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA).…”

Section: Determination Of Complete Bymv Genomes Using Htsmentioning

confidence: 99%

Molecular Characteristics and Biological Properties of Bean Yellow Mosaic Virus Isolates from Slovakia

Mrkvová,

Kemenczeiová,

Achs

et al. 2024

Horticulturae

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“…Subgroup I can be further divided into IA and IB. We included RNA3 sequences of 13 known CMV isolates representing CMV subgroups for the phylogenetic tree construction based on a previous study [11]. Although 21 CMV RNA sequences in this study were divided into two groups (group A and group B), all the 21 CMV RNA3 variants were grouped together with the 5 CMV isolates belonging to subgroup IA (Figure 7F).…”

Section: Viral Genome Assemblymentioning

confidence: 99%

Viromes of 15 Pepper (Capsicum annuum L.) Cultivars

Choi

Lee

et al. 2022

IJMS

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Pepper (Capsicum annuum L.) plants produce berry fruits that are used as spices. Here, we examined the viromes of 15 pepper cultivars through RNA sequencing. We obtained 1,325 virus-associated contigs derived from 8 virus species. Bean broad wilt virus 2 (BBWV2) and cucumber mosaic virus (CMV) were identified as the major viruses infecting pepper plants, followed by potato virus Y, bell pepper endornavirus, and hot pepper endornavirus. The proportion of viral reads in each transcriptome ranged from 0.04% to 24.5%. BBWV2 was the dominant virus in seven cultivars, whereas CMV was dominant in five cultivars. All the bell pepper cultivars showed severe viral disease symptoms, whereas the commercially developed hot pepper cultivars were asymptomatic or had mild symptoms. In addition, 111 complete viral segments were obtained from 7 viruses. Based on the obtained viral genomes, the genetic relationship between the identified viruses and quasispecies of BBWV2 and CMV in each pepper plant was determined. Newly designed primers for nine viruses confirmed the results of RNA sequencing. Taken together, this study, for the first time, provides a comprehensive overview of viromes in 15 major pepper cultivars through RNA sequencing.

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“…CMV isolates are classified into two major subgroups, termed I and II, according to their serological properties, symptomatology and CP sequence phylogenesis, and subgroup I is further divided into subgroup IA and IB based on RNA3 variability of the 5′ terminal noncoding region (Jacquemond, 2012; Roossinck et al, 1999). The host range of CMV contains the cultivated or wild plants (Mrkvová et al, 2022). In China, N. domestica was reported previously as a host of CMV subgroup II isolate (Wei et al, 2015), but it did not refer to the variety of the host, and the full genome sequences of the CMV isolate in N. domestica is unavailable.…”

Section: Introductionmentioning

confidence: 99%

Cucumber mosaic virus subgroup IA isolates infect four varieties of Nandina domestica in China

Du,

Luo,

Fei

et al. 2023

Journal of Phytopathology

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Cucumber mosaic virus (CMV) subgroup IA isolates were first identified from Nandina domestica plants in Zhejiang province of China by small RNA deep sequencing followed by bioinformatics analysis and was confirmed by RT‐PCR amplification, 5′ and 3′ RACE and DNA sequencing. The complete nucleotide sequences of this virus shared the highest sequence similarities (97–98%) with CMV subgroup IA isolate CTL RNA1 and RNA3 (GenBank accession no: EF213023, EF213025) and RP48 RNA2 (KC527727), respectively; and converged with CMV subgroup IA isolates into a branch in phylogenetic tree using full length nucleotide sequences. Pathogenicity test confirmed that this virus can be mechanically transmitted to N. domestica, Nicotiana tabacum and N. benthamiana. All thirty randomly collected samples of four major N. domestica varieties (N. domestica cv. Hong Baoshi, Nana, Fire Power and Xiu Dada) in Lin'an Zhejiang province were positive for CMV subgroup IA. This is the first report of CMV subgroup IA isolate on varieties of N. domestica in China.

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High-Throughput Sequencing Discloses the Cucumber Mosaic Virus (CMV) Diversity in Slovakia and Reveals New Hosts of CMV from the Papaveraceae Family

Cited by 6 publications

References 51 publications

Molecular Characteristics and Biological Properties of Bean Yellow Mosaic Virus Isolates from Slovakia

Molecular Characteristics and Biological Properties of Bean Yellow Mosaic Virus Isolates from Slovakia

Viromes of 15 Pepper (Capsicum annuum L.) Cultivars

Cucumber mosaic virus subgroup IA isolates infect four varieties of Nandina domestica in China

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