High-throughput sequencing analysis of microRNAs in gills of red swamp crayfish, Procambarus clarkii infected with white spot syndrome virus

Wang, Kai; Shen, Xiu-li; Jia, Jin-sheng; Yu, Xiaodong; Du, Jie; Lin, Sihan; Du, Zhiqiang

doi:10.1016/j.fsi.2018.09.007

Cited by 15 publications

(2 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Notedly, many immune related genes such as crustins, ALF, proPO were up-regulated in the pH group, which indicated that some pillar cells were in the immune-activated states after high pH stress. Although previous studies reported that the immune defense function of gills in crustacean was considered to be related to the plentiful hemolymph in this tissue, and we did identify the sessile hemocyte cluster of the gills in this study, there is no information about immune response function of the pillar cells ( Wang et al, 2018 ; Lv et al, 2020 ). This is the first report on the immune function of pillar cell except for ion transport.…”

Section: Discussionmentioning

confidence: 75%

Highly sensitive and specific responses of shrimp gill cells to high pH stress based on single cell RNA-seq analysis

Wang

et al. 2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

High pH is one of the main stressors affecting the shrimp survival, growth, and physiology in aquaculture ponds, but the cellular and molecular mechanism responsible for high pH stress has not been elucidated in shrimp. In this study, the shrimp acid-base disturbance and gill cell alterations were significantly observed and then single cell RNA-sequencing (scRNA-seq) was performed to study the sensitive and specific responses of gill cells to high pH stress. Three main gill cell types, including pillar cells, hemocytes and septal cells were identified. By comparative scRNA-seq analysis between control and pH group, the pillar cell was regarded as the target cell type in response to high pH stress with the down-regulation of ammonia excretion and H+ transport related genes and up-regulation of immune related genes. Notedly, high pH resulted in the emergence of a new immune cell subcluster in pillar cells, with immune activation and stress defense states. Pseudotime analysis also showed that the pillar cells could transform into the functionally inhibited ion cell subclusters and functionally activated immune cell subclusters after high pH stress. Further, the regulatory network of pillar cell population was predicted by WGCNA and two transcription factors were identified. In conclusion, these results provide key insights into the shrimp gill cell-type-specific mechanisms underlying high pH stress response at a single-cell resolution.

show abstract

Section: Discussionmentioning

confidence: 75%

Highly sensitive and specific responses of shrimp gill cells to high pH stress based on single cell RNA-seq analysis

Wang

et al. 2022

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

show abstract

“…Expression profile of miRNAs changed a lot in pathological state or environmental factor stimulation. Previous studies have reported the miRNA profiles in P. clarkia, for example, Wang et al and Du et al identified the miRNAs in gills, intestines and lymph organs of P. clarkia infected with white spot syndrome virus [13][14][15]. Ou et al screened the miRNAs potentially related to immunity against Spiroplasma eriocheiris infection in P. clarkia [16].…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of salt-tolerance relative miRNAs in Procambarus clarkii by high-throughput sequencing

Jin

Sun

2019

ExRNA

View full text Add to dashboard Cite

Procambarus clarkii is one of the important economic species in China and has been served as tasty food in recent years after being introduced to Nanjing. Significant problems of environment factors, such as salinity, pH and temperature, especially salinity, has the potential to result in significant economic losses in many crayfish-producing farms in China. miRNAs are a kind of~22 nucleotide small non coding RNAs which were encoded by plants, animals and some viruses with functions in RNA silencing or post-transcription regulation. We constructed four sRNA library of P. clarkia from different tissues and treatments by using high-throughput sequencing technology. A total of 101 conserved miRNAs and two novel pre-miRNAs were identified and RT-qPCR were further performed to confirm existence of part of identified miRNAs. A genome wide expression profile of salt-tolerance miRNAs was proved and three miRNAs were further validated by RT-qPCR with dynamic response to different salinity stages. The study of miRNAs in P. clarkia can help us better understanding the role of miRNAs in salt-tolerance in P. clarkia.

show abstract

Bisphenol S exposed changes in intestinal microflora and metabolomics of freshwater crayfish, Procambarus clarkii

Pu,

Liu,

et al. 2024

Aquatic Toxicology

View full text Add to dashboard Cite

High-throughput sequencing analysis of microRNAs in gills of red swamp crayfish, Procambarus clarkii infected with white spot syndrome virus

Cited by 15 publications

References 30 publications

Highly sensitive and specific responses of shrimp gill cells to high pH stress based on single cell RNA-seq analysis

Highly sensitive and specific responses of shrimp gill cells to high pH stress based on single cell RNA-seq analysis

Identification and characterization of salt-tolerance relative miRNAs in Procambarus clarkii by high-throughput sequencing

Bisphenol S exposed changes in intestinal microflora and metabolomics of freshwater crayfish, Procambarus clarkii

Contact Info

Product

Resources

About