2001
DOI: 10.1007/s00335-001-1001-x
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High-throughput sequence identification of gene coding variants within alcohol-related QTLs

Abstract: Low initial response to alcohol has been shown to be among the best predictors of development of alcoholism. A similar phenotypic measure, difference in initial sensitivity to ethanol, has been used for the genetic selection of two mouse strains, the Inbred Long-Sleep (ILS) and Inbred Short-Sleep (ISS) mice, and for the subsequent identification of four quantitative trait loci (QTLs) for alcohol sensitivity. We now report the application of high throughput comparative gene sequencing in the search for genes un… Show more

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Cited by 35 publications
(39 citation statements)
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“…Because ethanol LORR is mediated by multiple molecular mechanisms, it is likely that distinct molecular mechanisms are responsible for hypnotic effect of different doses of ethanol. For example, at least four quantitative trait loci on different mouse chromosomes and 23 candidate genes have been identified, which are largely responsible for the heritable component of hypnotic sensitivity to ethanol in selected ILS and ISS strains of mice (Markel et al, 1997;Bennett and Johnson, 1998;Ehringer et al, 2001;MacLaren et al, 2006). Furthermore, genotypic differences in duration of ethanol-induced LORR can reflect differences in sensitivity, acute tolerance or ethanol clearance (Radcliffe et al, 2005).…”
Section: Discussionmentioning
confidence: 99%
“…Because ethanol LORR is mediated by multiple molecular mechanisms, it is likely that distinct molecular mechanisms are responsible for hypnotic effect of different doses of ethanol. For example, at least four quantitative trait loci on different mouse chromosomes and 23 candidate genes have been identified, which are largely responsible for the heritable component of hypnotic sensitivity to ethanol in selected ILS and ISS strains of mice (Markel et al, 1997;Bennett and Johnson, 1998;Ehringer et al, 2001;MacLaren et al, 2006). Furthermore, genotypic differences in duration of ethanol-induced LORR can reflect differences in sensitivity, acute tolerance or ethanol clearance (Radcliffe et al, 2005).…”
Section: Discussionmentioning
confidence: 99%
“…Differentiated NT2-N cells with retinoic acid exhibited expression of KCC2, corroborating the presence of this transcript in a neuronal-derived cell line. KCC2 cDNA has been also isolated and sequenced from mouse by means of a highthroughput sequence study designed to identify gene-coding variants within alcohol-related QTLs in a mouse model of alcohol addiction (102) and also by the Mammalian Gene Collection Program Team (http//mgc.nci.nih.gov) (391).…”
Section: The Kmentioning
confidence: 99%
“…Preparation of PCR products from Creb1 cDNA was performed as described by Ehringer et al (2001). Briefly, C57 and DBA mice were sacrificed by cervical dislocation, and cerebellum was isolated and immediately frozen on liquid nitrogen.…”
Section: Qtls For Ethanol Sensitivity and Camp Signaling 1239mentioning
confidence: 99%
“…PCR products were sequenced directly as described by Ehringer et al (2001), using the ABI Prism Big Dye Terminator Sequencing Ready Reaction Kit (Applied Biosystems, Foster City, CA). Sequencing reactions were carried out as follows: 35 cycles of 10 s at 96°C, 50 s at 50°C, and 4 min at 60°C.…”
Section: Qtls For Ethanol Sensitivity and Camp Signaling 1239mentioning
confidence: 99%