High-throughput screening paradigms in ecotoxicity testing: Emerging prospects and ongoing challenges

Wlodkowic, Donald; Jansen, Marcus

doi:10.1016/j.chemosphere.2022.135929

Cited by 10 publications

(9 citation statements)

References 339 publications

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“…Microfluidics technology creates the potential for multiplexed analysis, single-cell, and gradient cytotoxicity assessments. Furthermore, this technology combines sensors and digital cameras for cytotoxicity studies with real-time data collection, allowing the monitoring of many cellular parameters, such as mortality, cell-substrate adhesion, electrophysiology, cell division and kinetics responses of cytotoxicity in a label-free manner (Garcia-Hernando et al, 2020;Wlodkowic and Jansen, 2022).…”

Section: Innovative Approaches To Assessing Nms Toxicity Using Daphni...mentioning

confidence: 99%

Daphnia as a model organism to probe biological responses to nanomaterials—from individual to population effects via adverse outcome pathways

et al. 2023

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The importance of the cladoceran Daphnia as a model organism for ecotoxicity testing has been well-established since the 1980s. Daphnia have been increasingly used in standardised testing of chemicals as they are well characterised and show sensitivity to pollutants, making them an essential indicator species for environmental stress. The mapping of the genomes of D. pulex in 2012 and D. magna in 2017 further consolidated their utility for ecotoxicity testing, including demonstrating the responsiveness of the Daphnia genome to environmental stressors. The short lifecycle and parthenogenetic reproduction make Daphnia useful for assessment of developmental toxicity and adaption to stress. The emergence of nanomaterials (NMs) and their safety assessment has introduced some challenges to the use of standard toxicity tests which were developed for soluble chemicals. NMs have enormous reactive surface areas resulting in dynamic interactions with dissolved organic carbon, proteins and other biomolecules in their surroundings leading to a myriad of physical, chemical, biological, and macromolecular transformations of the NMs and thus changes in their bioavailability to, and impacts on, daphnids. However, NM safety assessments are also driving innovations in our approaches to toxicity testing, for both chemicals and other emerging contaminants such as microplastics (MPs). These advances include establishing more realistic environmental exposures via medium composition tuning including pre-conditioning by the organisms to provide relevant biomolecules as background, development of microfluidics approaches to mimic environmental flow conditions typical in streams, utilisation of field daphnids cultured in the lab to assess adaption and impacts of pre-exposure to pollution gradients, and of course development of mechanistic insights to connect the first encounter with NMs or MPs to an adverse outcome, via the key events in an adverse outcome pathway. Insights into these developments are presented below to inspire further advances and utilisation of these important organisms as part of an overall environmental risk assessment of NMs and MPs impacts, including in mixture exposure scenarios.

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Section: Innovative Approaches To Assessing Nms Toxicity Using Daphni...mentioning

confidence: 99%

Daphnia as a model organism to probe biological responses to nanomaterials—from individual to population effects via adverse outcome pathways

et al. 2023

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“…Fundamental within the evolving topic of NGRA in many regulatory areas are new approach methods (NAMs). , Historically, NAMs were intended for mechanistic hazard characterization in the 3R vertebrate domain . Recently, these approaches have been extended beyond their original narrow phylogenetic scope to a broad range of organisms . In contrast to traditional tests that focus on quantifying apical effects, NAMs aim to characterize dose–response effects in novel and traditional test systems.…”

Section: Introductionmentioning

confidence: 99%

“… 6 Recently, these approaches have been extended beyond their original narrow phylogenetic scope to a broad range of organisms. 7 In contrast to traditional tests that focus on quantifying apical effects, NAMs aim to characterize dose–response effects in novel and traditional test systems. They comprise a heterogeneous collection of approaches that include a variety of in vitro experimental setups (e.g., cell lines and embryos), experimental readouts (OMICs, e.g., transcriptomics, proteomics, and metabolomics), and in silico methods (e.g., Bayesian networks and machine learning applications).…”

Section: Introductionmentioning

confidence: 99%

“…They comprise a heterogeneous collection of approaches that include a variety of in vitro experimental setups (e.g., cell lines and embryos), experimental readouts (OMICs, e.g., transcriptomics, proteomics, and metabolomics), and in silico methods (e.g., Bayesian networks and machine learning applications). Further, NAMs offer potential for implementation within high-throughput applications to characterize adverse effects at scale (for a detailed view on NAMs, see 7 ).…”

Section: Introductionmentioning

confidence: 99%

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Missing Genomic Resources for the Next Generation of Environmental Risk Assessment

Roell,

Ott,

Mair

et al. 2024

Environ. Sci. Technol.

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Environmental risk assessment traditionally relies on a wide range of in vivo testing to assess the potential hazards of chemicals in the environment. These tests are often time-consuming and costly and can cause test organisms' suffering. Recent developments of reliable low-cost alternatives, both in vivo-and in silico-based, opened the door to reconsider current toxicity assessment. However, many of these new approach methodologies (NAMs) rely on high-quality annotated genomes for surrogate species of regulatory risk assessment. Currently, a lack of genomic information slows the process of NAM development. Here, we present a phylogenetically resolved overview of missing genomic resources for surrogate species within a regulatory ecotoxicological risk assessment. We call for an organized and systematic effort within the (regulatory) ecotoxicological community to provide these missing genomic resources. Further, we discuss the potential of a standardized genomic surrogate species landscape to enable a robust and nonanimal-reliant ecotoxicological risk assessment in the systems ecotoxicology era.

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“…Historically, NAMs were intended for mechanistic hazard characterization in the 3R vertebrate domain 6 . Recently, these approaches have been extended beyond their original narrow phylogenetic scope to a broad range of organisms 7 . NAMs deviate from traditional dose-response toxicity tests.…”

Section: Introductionmentioning

confidence: 99%

Missing genomic resources for the next generation of environmental risk assessment

Roell,

Ott,

Mair

et al. 2023

Preprint

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Environmental risk assessment traditionally relies on a wide range of in vivo testing to assess the potential hazard of chemicals in the environment. These tests are often time-consuming, costly and can cause test organism suffering. Recent developments of reliable low-cost alternatives, both in vivo- and in silico-based, opened the door to reconsider current toxicity assessment. However, many of these new approach methodologies (NAMs) rely on high quality annotated genomes for surrogate species of the regulatory risk assessment. Currently, lacking genomic information slows the process of NAM development. Here, we present a phylogenetically resolved overview of missing genomic resources for surrogate species within regulatory ecotoxicological risk assessment. We call for an organized and systematic effort within the (regulatory) ecotoxicological community to provide these missing genomic resources. Further, we discuss the potential of a standardized genomic surrogate species landscape to enable a robust and non-animal reliant ecotoxicological risk assessment in the systems ecotoxicology era.

show abstract

High-throughput screening paradigms in ecotoxicity testing: Emerging prospects and ongoing challenges

Cited by 10 publications

References 339 publications

Daphnia as a model organism to probe biological responses to nanomaterials—from individual to population effects via adverse outcome pathways

Daphnia as a model organism to probe biological responses to nanomaterials—from individual to population effects via adverse outcome pathways

Missing Genomic Resources for the Next Generation of Environmental Risk Assessment

Missing genomic resources for the next generation of environmental risk assessment

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