High-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag

Data for high-throughput screening of enzyme mutants by comparison of their activity ratios to an enzyme tag

“…1a, b, c, d in Ref. [1], respectively, here are the detection of polypeptides after separation by PAGE. (a) Coomassie Blue R250 staining.…”

“…The dataset in this report will facilitate understanding the validation and application of a new high-throughput screening strategy to mutants of an enzyme in a library [1]. To recognize positive mutants in a library, this new screening strategy fuses enzyme/mutants with a tag enzyme to compare activity ratios of the enzyme/mutants to the tag enzyme in cell lysates of their fused forms, when such activity ratios have physical significance and are proportional to specific activities of the non-fused counterpart enzyme/mutants.…”