High-Throughput Screening of Cell Repellent Substrate Chemistry for Application in Expanded Bed Adsorption Chromatography

Yelemane, Vikas; Kangwa, Martin; Fernández‐Lahore, Marcelo

doi:10.1007/978-981-10-1920-3_16

Cited by 2 publications

(2 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cultivation of yeast cells, Saccharomyces cerevisiae (S. cerevisiae) was performed using shake asks, harvested at late exponential phase, as described elsewhere [16].…”

Section: Microbial and Cell Culturesmentioning

confidence: 99%

See 1 more Smart Citation

Convenient Screening of Antifouling Agents for Direct Recovery of Bioproducts Employing a Fast Microplate Assay

Yelemane¹,

Kangwa²,

Dsouza³

et al. 2020

Preprint

Self Cite

View full text Add to dashboard Cite

Major limitations in direct recovery of bioproducts from unclarified feedstock are predominantly associated with the presence of suspended whole and lysed biomass in processing systems. Most commonly, biomass interaction with adsorbent beads, synthetic membranes, and other processes surfaces causes fouling. This phenomenon is usually detrimental to bioprocess performance. This study focused on the development of a simple, easy to implement, economical and robust technique to evaluate biomass deposition and screening for chemical agents able to prevent such phenomenon. The results were confirmed by linking biomass deposition with surface energetics according to the extended Derjaguin, Landau, Verwey and Overbeek (xDLVO) theory. Assay development involved the modification of the inside surface of microwell polystyrene plates with diethyl-aminoethyl (DEAE) functional groups. The resulting microplate surface mimics commercial chromatographic anion-exchangers matrices that are prone to biomass fouling. Two biomass types, Saccharomyces cerevisiae and Chinese hamster ovary (S. cerevisiae and CHO, respectively) cells and twenty-five polyelectrolytes or amphiphilic compounds were employed. Results showed that DEAE-modified surface had the highest cell deposition while agent-coated wells showed varying degrees. Direct experimental observations and calculations performed based on xDLVO approach indicated that polyacrylic acid, polymethacrylic acid, and poly(vinyl sulfate) could prevent CHO cells and yeast deposition. On the other hand, polysulphonic acid and poly(sodium 4-styrenesulfonate) were only effective to prevent yeast deposition. Microscopic visualization of polymer-coated beads in the presence of biomass confirmed the mentioned results.

show abstract

“…Cultivation of yeast cells, Saccharomyces cerevisiae (S. cerevisiae) was performed using shake asks, harvested at late exponential phase, as described elsewhere [16].…”

Section: Microbial and Cell Culturesmentioning

confidence: 99%

“…The detailed methodology for the modi cation of microwell plate is described elsewhere [16]. Brie y, PGMA was synthesized by homo-polymerisation of glycidyl methacrylate in methanol with 2,2′-Azobis(2methylpropionitrile) as an initiator.…”

Section: Modi Cation Of Microwells With Deae-dextranmentioning

confidence: 99%