High-throughput screening of a nicotinate dehydrogenase producing Pseudomonas putida mutant for efficient biosynthesis of 6-hydroxynicotinic acid

Shang, Yan; Qin, Jiufu; Gong, Jin Song; Wang, Zi Kai; Li, Heng; Li, Hui; Shi, Jin Song; Xu, Zhongyu

doi:10.1016/j.mcat.2021.111600

Cited by 6 publications

(3 citation statements)

References 29 publications

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“…3a). In previous studies, screening samples were enriched and fermented, and the fermentation droplets were subsequently observed and developed on lter paper [7,9]. Compared to previous investigations, this study eliminates the need for labor-intensive fermentation liquid culture, allowing for the rapid identi cation of target enzyme-producing strains.…”

Section: Strain Identi Cationmentioning

confidence: 99%

“…Shang et al [7] reported that organic acids such as pyruvate and fumaric acid, when used as fermentation carbon sources, can enhance the increase in nicotinic acid dehydrogenase activity. In contrast to these ndings, the nicotinic acid dehydrogenase activity using soluble starch in this study was higher than that of other carbon sources, and pyruvate was not considered a favorable carbon source.…”

Section: Effect Of Different Carbon Sourcesmentioning

confidence: 99%

“…Most reported biosynthesis methods for 6-hydroxynicotinic acid involve the catalysis of nicotinic acid by nicotinic acid dehydrogenase produced by Pseudomonas strains [7]. The production of 6-HNA is directly in uenced by the activity of nicotinic dehydrogenase [8].…”

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confidence: 99%

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Efficient production of 6-Hydroxynicotinic Acid by newly isolated Pseudomonas poae

Li,

Tang,

et al. 2024

Preprint

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Nicotinic acid dehydrogenase possesses the capability to convert nicotinic acid into 6-hydroxynicotinic acid, a compound of significant research value as a pharmaceutical intermediate. The extraction of nicotinic acid dehydrogenase is primarily performed by strains. However, the enzyme activity of the strains reported currently is relatively low, and their potential to catalyze the production of 6-hydroxynicotinic acid is insufficient to meet industrial requirements. Due to the revealing properties of 6-hydroxynicotinic acid, this study proposes a technique for calculating the luminescence intensity of colonies, which is based on a fluorescence spectrometer. The developed method establishes a reliable linear relationship (88.2%) between the luminescence intensity and enzyme activity. Consequently, it has been employed to screen strains that produce nicotinate dehydrogenase. This screening approach allows for the evaluation of about 500 enzyme-producing strains daily, presenting an efficient strategy for screening. Through this approach, a novel high enzyme activity strain producing nicotinic acid dehydrogenase, Pseudomonas poae have been obtained, which designated as HD530. After process optimization, it was utilized to produce 6-hydroxynicotinic acid, achieving a high yield of 155.45 g/L within 72 hours, meeting the requirements for industrial production. The effectiveness and potential of this technique lie in its application for strain screening and improvement.

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Section: Strain Identi Cationmentioning

confidence: 99%

Section: Effect Of Different Carbon Sourcesmentioning

confidence: 99%