High-throughput screening: new technology for the 21st century

Hertzberg, Robert; Pope, Andrew J.

doi:10.1016/s1367-5931(00)00110-1

Cited by 494 publications

(294 citation statements)

References 62 publications

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“…However, miniaturisation of assays leads to a series of key challenges, reviewed by a range of authors late in the 20th century (Hertzberg and Pope 2000, Sittampalam et al 1997, Silverman et al 1998, Lemmo et al 1998 such as (i) assay methods and detection, (ii) liquid handling and robotics and (iii) process flow and information management. It is the second challenge for which inkjet printing was identified as a potentially important tool (Blanchard et al 1996, Lemmo et al 1997, Sittampalam et al 1997, Burbaum et al 1997, Lemmo et al 1998, Oldenburg et al 1998, Schena et al 1998, Rose 1999, Dunn and Feygin 2000, Bellavance et al 2000, Taylor et al 2002.…”

Section: High Throughput 'System Discovery' Techniquesmentioning

confidence: 99%

Inkjet printing for pharmaceutics – A review of research and manufacturing

Daly

Harrington

Martin

et al. 2015

International Journal of Pharmaceutics

258

185

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Abstract:Global regulatory, manufacturing and consumer trends are driving a need for change in current pharmaceutical sector business models, with a specific focus on the inherently expensive research costs, high-risk capital-intensive scale-up and the traditional centralised batch manufacturing paradigm. New technologies, such as inkjet printing, are being explored to radically transform pharmaceutical production processing and the end-to-end supply chain. This review provides a brief summary of inkjet printing technologies and their current applications in manufacturing before examining the business context driving the exploration of inkjet printing in the pharmaceutical sector. We then examine the trends reported in the literature for pharmaceutical printing, followed by the scientific considerations and challenges facing the adoption of this technology. We demonstrate that research activities are highly diverse, targeting a broad range of pharmaceutical types and printing systems. To mitigate this complexity we show that by categorising findings in terms of targeted business models and Active Pharmaceutical Ingredient (API) chemistry we have a more coherent approach to comparing research findings and can drive efficient translation of a chosen drug to inkjet manufacturing.

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Section: High Throughput 'System Discovery' Techniquesmentioning

confidence: 99%

Inkjet printing for pharmaceutics – A review of research and manufacturing

Daly

Harrington

Martin

et al. 2015

International Journal of Pharmaceutics

258

185

View full text Add to dashboard Cite

show abstract

“…In order to give rapid and effective assay to the huge pool of molecules, enormous efforts have been put into the large-scale automation of high-throughput screening (HTS) [2]. It does not mean that the combination of combinatorial chemistry and HTS will spell the end of any rational and knowledge-based approach, this is because initial euphoria that designated this technique as a universal lead generator has subsided as a result of the considerable costs involved and disappointingly low hit rates.…”

Section: Introductionmentioning

confidence: 99%

Recent Development and Application of Virtual Screening in Drug Discovery: An Overview

Hou¹,

Xu²

2012

Frontiers in Medicinal Chemistry - (Volume 3)

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Virtual screening, especially the structure-based virtual screening, has emerged as a reliable, cost-effective and time-saving technique for the discovery of lead compounds. Here, the basic ideas and computational tools for virtual screening have been briefly introduced, and emphasis is placed on aspects of recent development of docking-based virtual screening, scoring functions in molecular docking and ADME/Tox-based virtual screening in the past three years (2000 to 2003). Moreover, successful examples are provided to further demonstrate the effectiveness of virtual screening in drug discovery.

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“…High-throughput screening assays play a pivotal role in the search of novel drugs and potential therapeutics. Over the last decade, a variety of scientific advances include the growing number of potential therapeutic targets emerging from the field of functional genomics and the rapid development of large compound libraries derived from parallel and combinatorial chemical synthetic techniques, driven the need for improved drug discovery screening technology [3,4]. Testing very small samples is very attractive because it requires only minute amounts of both the component being assayed and the components of the assay, both of which may be quite expensive or available in very limited amounts [5].…”

Section: Introductionmentioning

confidence: 99%

Optimization of fluorescent cell-based assays for high-throughput analysis using microchamber array chip formats

Akagi

Rao

Morita

et al. 2004

Science and Technology of Advanced Materials

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This study describes the optimization of fluorescent cell-based assays using microchamber array chip formats as well as using automatic nanoliter volumes of sample dispensing system for high-throughput screening analysis of anticancer drugs. Cell-based assays can be employed efficiently in the screening of potential anticancer drug candidates and bioactive compounds with distinct biological function. Identification and development of cell-based assays adapted to high-throughput screening requirements is important when screening chemicals for their potential anticancer properties. Cell-based screening assays using microchamber array chip formats and automatic nanoliter volumes of sample dispensing system requires an optimization as a prerequisite for parameters including assay liquid volume and number of cells per each chamber, and the total cell-based assay itself. Further, the anticancer effect of mitomycin C was studied as an example against human cervical carcinoma cell line-HeLa 229 using cell-based assay that was optimized on chamber array chip formats and determined the cytotoxicity of mitomycin C by measuring the cell proliferation of HeLa with Calcein-AM fluorescent dye. The cell-based screening assay that was performed using chamber array chip formats was compared with the conventional 96-well plate formats was discussed. The assay described in this study is rapid, simple and inexpensive that is desirable in selecting anticancer candidates. q

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High-throughput screening: new technology for the 21st century

Cited by 494 publications

References 62 publications

Inkjet printing for pharmaceutics – A review of research and manufacturing

Inkjet printing for pharmaceutics – A review of research and manufacturing

Recent Development and Application of Virtual Screening in Drug Discovery: An Overview

Optimization of fluorescent cell-based assays for high-throughput analysis using microchamber array chip formats

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