High-throughput screening for drugs of abuse and pharmaceutical drugs in hair by liquid-chromatography-high resolution mass spectrometry (LC-HRMS)

Odoardi, Sara; Valentini, Valeria; Giovanni, Nadia De; Pascali, Vincenzo Lorenzo; Rossi, Sabina Strano

doi:10.1016/j.microc.2017.03.050

Cited by 43 publications

(27 citation statements)

References 26 publications

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“…The method was successfully applied to forensic case studies. A rapid screening method for the multi‐analyte drug analysis in hair samples, including PGB, was recently proposed by Odoardi et al By means of a liquid chromatography‐high‐resolution mass spectrometry (LC–HRMS) apparatus, the authors were able to simultaneously determine over 170 drugs for forensic and clinical toxicology investigations. Interesting studies dealing with both spectrophotometric and spectrophluorimetric methods for the determination of PGB were also described.…”

Section: Resultsmentioning

confidence: 99%

GC‐MS/MS detects potential pregabalin abuse in susceptible subjects’ hair

Ianni

Aroni

Gili

et al. 2018

Drug Testing and Analysis

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Pregabalin, a GABA analogue, binds to the alpha 2 delta subunit of voltage-dependent calcium channels. It is recognised as efficacious in pathologies such as epilepsy, neuropathic pain, and anxiety disorders. Since pregabalin prescriptions have increased worldwide, reports of its abuse have been accumulating, mainly in patients with opioid abuse disorders. The present study investigated potential pregabalin abuse by means of hair analysis, a matrix that provides valuable retrospective information. Half of the pool of 280 susceptible patients had been occasional drug users and were being monitored for driving licence renewals. The other 140 patients had a history of opiate dependency and were monitored to assess compliance with methadone therapy. In view of determining pregabalin in hair samples, it was extracted in methanol, successfully derivatised to give the ethyl chloroformate derivative, and finally pregabalin was analysed by gas chromatography-tandem mass spectrometry. Selectivity, linearity, limit of detection, limit of quantification, recovery, intra- and inter-day precision, and accuracy of the quantification procedure were appraised. Pregabalin limits of detection and quantification were 30 pg/mg and 50 pg/mg, respectively. We found 10.7% of hair samples from methadone patients and 4.29% from occasional drug users were positive to pregabalin without medical prescription. The mean pregabalin concentration in hair was higher than in consumers with medical indications (1.45 ng/mg vs 0.74 ng/mg). These results suggest that pregabalin possesses a significant abuse potential particularly among individuals attending opiate dependence services and that pregabalin abuse is a serious emerging issue, which should be carefully monitored.

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Section: Resultsmentioning

confidence: 99%

GC‐MS/MS detects potential pregabalin abuse in susceptible subjects’ hair

Ianni

Aroni

Gili

et al. 2018

Drug Testing and Analysis

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“…Marijuana or hashish, a cocktail of at least 30 different major cannabinoids, generally prepared from crushing the leaves, flowers (inflorescenses) and even stems of C. sativa , is one of the oldest recreational and addictive natural products used by humans for centuries . However, the non‐medical use of Cannabis or marijuana is illegal in many countries, which prompts the use of analytical tools, like HPLC and UPLC, to analyse biological and forensic samples like blood, oral liquid, hair and urine, to confirm marijuana usage (Tables ) . After consumption of marijuana, THCA ( 13 ), which is present in the crude marijuana, but converted to THC ( 12 ) by heat during smoking, is excreted in the urine as its glucuronide conjugate, and can be analysed comfortably by LC methods.…”

Section: Hplc and Uplc (Or Uhplc) Analysis Of Naturally Occurring Canmentioning

confidence: 99%

“…While there have been several HPLC‐based methods reported during the past decade, the use of UPLC‐based analysis for hair samples is rather limited to the publication by Shah et al . (Table ).…”

Section: Hplc and Uplc (Or Uhplc) Analysis Of Naturally Occurring Canmentioning

confidence: 99%

A review on the recent advances in HPLC, UHPLC and UPLC analyses of naturally occurring cannabinoids (2010–2019)

Nahar

Önder

Sarker

2019

Phytochemical Analysis

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Introduction Organic molecules that bind to cannabinoid receptors are called cannabinoids, and they have similar pharmacological properties like the plant, Cannabis sativa L. Hyphenated liquid chromatography (LC), incorporating high‐performance liquid chromatography (HPLC) and ultra‐performance liquid chromatography (UPLC, also known as ultrahigh‐performance liquid chromatography, UHPLC), usually coupled to an ultraviolet (UV), UV‐photodiode array (PDA) or mass spectrometry (MS) detector, has become a popular analytical tool for the analysis of naturally occurring cannabinoids in various matrices. Objective To review literature on the use of various LC‐based analytical methods for the analysis of naturally occurring cannabinoids published since 2010. Methodology A comprehensive literature search was performed utilising several databases, like Web of Knowledge, PubMed and Google Scholar, and other relevant published materials including published books. The keywords used, in various combinations, with cannabinoids being present in all combinations, in the search were Cannabis, hemp, cannabinoids, Cannabis sativa, marijuana, analysis, HPLC, UHPLC, UPLC, quantitative, qualitative and quality control. Results Since 2010, several LC methods for the analysis of naturally occurring cannabinoids have been reported. While simple HPLC‐UV or HPLC‐UV‐PDA‐based methods were common in cannabinoids analysis, HPLC‐MS, HPLC‐MS/MS, UPLC (or UHPLC)‐UV‐PDA, UPLC (or UHPLC)‐MS and UPLC (or UHPLC)‐MS/MS, were also used frequently. Applications of mathematical and computational models for optimisation of different protocols were observed, and pre‐analyses included various environmentally friendly extraction protocols. Conclusions LC‐based analysis of naturally occurring cannabinoids has dominated the cannabinoids analysis during the last 10 years, and UPLC and UHPLC methods have been shown to be superior to conventional HPLC methods.

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“…In the literature, numerous studies about hair analysis have been increased over time, especially in the toxicological, clinical, and doping control area. However, there are other applications that use hair for the investigation of illegal drugs . For instance, according to Jurado et al, hair bleaching and/or dyeing can mask drug concentrations …”

Section: Introductionmentioning

confidence: 99%

Wavelength dispersive X‐ray fluorescence (WDXRF) and chemometric investigation of human hair after cosmetic treatment

Santos¹,

Sperança

Pereira³

2018

X-Ray Spectrometry

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Variations in the chemical composition of 63 different human and 6 different synthetic hair samples were investigated using wavelength dispersive X‐ray fluorescence (WDXRF) spectra profiles. To evaluate the effect of cosmetic treatment on the strands, the human hair samples were bleached, but the synthetic ones were not. To better investigate the data, exploratory analyses were calculated using principal component analysis for the WDXRF spectra. Eight normalizations/standardizations were applied in the WDXRF to verify the clustering tendency. Bleaching was tested, because it is one way in which people mask their real hair color. After the data were standardized, an enhancement of the data discrimination was verified. Furthermore, the explained variance was higher in the first principal components. The WDXRF spectra were able to distinguish samples with distinct features, including synthetic, dyed, and straightened hair. The findings of this study hold promise for forensics due to desirable aspects such as nondestructivity and the possibility of a large hair sample database.

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High-throughput screening for drugs of abuse and pharmaceutical drugs in hair by liquid-chromatography-high resolution mass spectrometry (LC-HRMS)

Cited by 43 publications

References 26 publications

GC‐MS/MS detects potential pregabalin abuse in susceptible subjects’ hair

GC‐MS/MS detects potential pregabalin abuse in susceptible subjects’ hair

A review on the recent advances in HPLC, UHPLC and UPLC analyses of naturally occurring cannabinoids (2010–2019)

Wavelength dispersive X‐ray fluorescence (WDXRF) and chemometric investigation of human hair after cosmetic treatment

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