High Throughput Screening for Bioactive Components of Berberis baluchistanica Ahrendt Root and Their Functional Potential Assessment

Abstract

Berberis baluchistanica Ahrendt is a medicinal plant potentially known for the treatment of different diseases. The bioactive, antioxidant, nutritional components, and antimicrobial properties of crude ethanolic root extract of Berberis baluchistanica were evaluated in this study. The extract was analyzed for total phenolic, flavonoid, DPPH (2, 2-diphenyl-1-picryl-hydrazyl) scavenging ability, FRAP (ferric reducing antioxidant power), nutritional, and antimicrobial potentials. The alkaloids, tannins, cardiac glycosides, anthraquinones, coumarin, saponins, phenolics, flavonoids, steroids, and terpenoids were confirmed. The extract possessed DPPH radical inhibition with the IC50 of 1.125 mg/mL and FRAP % reduction activity with IC50 (0.912 mg/mL). Total phenolic 19.897 ± 4.8141 mg GAE/g and flavonoid 12.9876 ± 0.8388 mg QE/g contents were confirmed in the root. The extracts exhibit good antibacterial activity against a broad spectrum of food borne pathogens Pseudomonas aeruginosa, Salmonella typhi, Klebsiella pneumoniae, Escherichia coli, and Staphylococcus aureus. The highest inhibitory activity was against Escherichia coli 23.30 ± 1.16 mm and lowest against Klebsiella pneumoniae 7 ± 0.01 mm . Furthermore, the presence of various phytochemical constituents (plant secondary metabolites) was also confirmed with gas chromatography and mass spectroscopy analysis. Results disclosed the occurrence of more than 70 compounds possessing various medicinal properties supporting the traditional uses of root of Berberis baluchistanica in various medical complications indigenously.

High throughput biochemical profiling, and functional potential analysis for valorization of grape peduncles

“…Due to their redox properties, phenolic and flavonoid contents which are abundant in plants have been discovered to have antispasmodic, antibacterial, anti-inflammatory, anticancer, antifungal, antidepressant and antioxidant power 16 . Phenolic compounds regulate plant cell division and development, also stabilize lipids against peroxidation and neutralize metabolic processes by scavenging free radicals.…”

“…According to Kadhim et al 37 2-Furanmethanol has the strong anti-inflammatory activity which was isolated from methanolic extract of grape. Hexadecanoic acid has potent antimicrobial activity and Hexasiloxane tetradecamethyl is a strong anticancer agent while Octadecadienoic acid (Z, Z)—has powerful anti-inflammatory, and anti-diabetic power 16 . Carvacrol derivative has high as antimicrobial, antimutagenic, anti-inflammatory, antitumor, antiparasitic activity 38 .…”

“…Applications for oleic acid include medicines, food additive and emulsifier, while lactic acid, 2TMS derivative used as antibacterial, keratolytic and emollient agent in various cosmetic material 39 . Erythritol is a sweet antioxidant releases oxidative stress and has unique nutritional effects 16 . The significant biological and pharmacological effects of resulted compounds have already been documented by Rani et al 32 .…”

“…The qualitative analysis of phytochemicals included carbohydrates, alkaloids, cardiac glycoside, coumarin, saponin, steroids, tannin, quinine, terpenoid, flavonoid and phenol in EGP extract was carried out by the methodology explained by Gul et al 16 .…”