High-throughput screening for analysis of in vitro toxicity

Schoonen, W.G.E.J.; Westerink, Walter M.A.; Horbach, G. Jean

doi:10.1007/978-3-7643-8336-7_14

Cited by 34 publications

(31 citation statements)

References 197 publications

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“…[30][31][32]62,67 Compared with the blank control, neither sublethal toxicity nor drop of hatching rate was detectable in the presence of lGO/NPcurcumin complexes. Screening of phenotypic toxicity may be implemented because of opaque quality of tissues following cell death.…”

Section: ■ Results and Discussionmentioning

confidence: 92%

Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model

et al. 2013

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In this paper, a facile strategy to develop graphenebased delivery nanosystems for effective drug loading and sustained drug release was proposed and validated. Specifically, biocompatible naphthalene-terminated PEG (NP) and anticancer drugs (curcumin or doxorubicin (DOX)) were simultaneously integrated onto oxidized graphene (GO), leading to selfassembled, nanosized complexes. It was found that the oxidation degree of GO had a significant impact on the drug-loading efficiency and the structural stability of nanosystems. Interestingly, the nanoassemblies resulted in more effective cellular entry of DOX in comparison with free DOX or DOX-loaded PEGpolyester micelles at equivalent DOX dose, as demonstrated by confocal microscopy studies. Moreover, the nanoassemblies not only exhibited a sustained drug release pattern without an initial burst release, but also significantly improved the stability of formulations which were resistant to drug leaking even in the presence of strong surfactants such as aromatic sodium benzenesulfonate (SBen) and aliphatic sodium dodecylsulfonate (SDS). In addition, the nanoassemblies without DOX loading showed negligible in vitro cytotoxicity, whereas DOX-loaded counterparts led to considerable toxicity against HeLa cells. The DOX-mediated cytotoxicity of the graphene-based formulation was around 20 folds lower than that of free DOX, most likely due to the slow DOX release from complexes. A zebrafish model was established to assess the in vivo safety profile of curcumin-loaded nanosystems. The results showed they were able to excrete from the zebrafish body rapidly and had nearly no influence on the zebrafish upgrowth. Those encouraging results may prompt the advance of graphene-based nanotherapeutics for biomedical applications.

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Section: ■ Results and Discussionmentioning

confidence: 92%

Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model

et al. 2013

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“…The next step involves feasibility and bioactivity testing and should be carried out in vitro and in vivo. In vitro assays using cell culture preparations are used to characterise the effects of materials on isolated cell function and for screening large numbers of compounds for biological activity, toxicity and immunogenicity (193)(194). However, due to their nature using isolated cells, in vitro models are unavoidably limited in their capacity to reflect complex in vivo environments that the TEC will be exposed to and are therefore inadequate to predict in vivo or clinical performances.…”

Section: Rationale For Translating Bone Tissue Engineering Strategiesmentioning

confidence: 99%

Bone Regeneration Based on Tissue Engineering Conceptions — A 21st Century Perspective

et al. 2013

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The role of Bone Tissue Engineering in the field of Regenerative Medicine has been the topic of substantial research over the past two decades. Technological advances have improved orthopaedic implants and surgical techniques for bone reconstruction. However, improvements in surgical techniques to reconstruct bone have been limited by the paucity of autologous materials available and donor site morbidity. Recent advances in the development of biomaterials have provided attractive alternatives to bone grafting expanding the surgical options for restoring the form and function of injured bone. Specifically, novel bioactive (second generation) biomaterials have been developed that are characterised by controlled action and reaction to the host tissue environment, whilst exhibiting controlled chemical breakdown and resorption with an ultimate replacement by regenerating tissue. Future generations of biomaterials (third generation) are designed to be not only osteoconductive but also osteoinductive, i.e. to stimulate regeneration of host tissues by combining tissue engineering and in situ tissue regeneration methods with a focus on novel applications. These techniques will lead to novel possibilities for tissue regeneration and repair. At present, tissue engineered constructs that may find future use as bone grafts for complex skeletal defects, whether from post-traumatic, degenerative, neoplastic or congenital/developmental "origin" require osseous reconstruction to ensure structural and functional integrity. Engineering functional bone using combinations of cells, scaffolds and bioactive factors is a promising strategy and a particular feature for future development in the area of hybrid materials which are able to exhibit suitable biomimetic and mechanical properties. This review will discuss the state of the art in this field and what we can expect from future generations of bone regeneration concepts.

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“…Since the results of LDH release assays compared favorably with those from conventional 51 Cr release assays, the latter is no longer in use (3). Due to the ubiquitous nature of LDH, this assay is now widely used for measuring acute cytotoxicity of any chemical in other cell types.…”

Section: Introductionmentioning

confidence: 98%

Cellular Chemosensitivity Assays: An Overview

Sumantran

2011

Methods in Molecular Biology

142

141

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Data on cell viability have long been obtained from in vitro cytotoxicity assays. Today, there is a focus on markers of cell death, and the MTT cell survival assay is widely used for measuring cytotoxic potential of a compound. However, a comprehensive evaluation of cytotoxicity requires additional assays which -measure short and long-term cytotoxicity. Assays which measure the cytostatic effects of compounds are not less important, particularly for newer anticancer agents. This overview discusses the advantages and disadvantages of different non-clonogenic assays for measuring short and medium-term cytotoxicity. It also discusses clonogenic assays, which accurately measure long-term cytostatic effects of drugs and toxic agents. For certain compounds and cell types, the advent of high throughput, multiparameter, cytotoxicity assays, and gene expression assays have made it possible to predict cytotoxic potency in vivo.

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High-throughput screening for analysis of in vitro toxicity

Cited by 34 publications

References 197 publications

Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model

Graphene-Based Anticancer Nanosystem and Its Biosafety Evaluation Using a Zebrafish Model

Bone Regeneration Based on Tissue Engineering Conceptions — A 21st Century Perspective

Cellular Chemosensitivity Assays: An Overview

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