High-Throughput Salting-Out Assisted Liquid/Liquid Extraction and Ultrafast LC for Same-Day Delivery of First-in-Human Bioanalytical Data

Zhang, Jun; Myasein, Francis; Rodila, Ramona; Wu, Huaiqin; El-Shourbagy, Tawakol A.

doi:10.4155/bio.09.71

Cited by 14 publications

(7 citation statements)

References 13 publications

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“…Plasma concentrations of ABT‐436 were determined using a validated liquid chromatography method with tandem mass spectrometric (Zhang et al., ). Deuterated ABT‐436 (ABT‐436‐D8) was used as an internal standard.…”

Section: Methodsmentioning

confidence: 99%

Single‐Dose Interaction Study of the Arginine Vasopressin Type 1B Receptor Antagonist ABT‐436 and Alcohol in Moderate Alcohol Drinkers

Katz

Locke

Liu

et al. 2016

Alcoholism Clin & Exp Res

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Background: ABT-436, a potent and selective arginine vasopressin (AVP) type 1B receptor (V 1B ) antagonist, has previously demonstrated basal hypothalamic-pituitary-adrenal (HPA) axis attenuation in man. A V 1B antagonist is hypothesized as an alcohol-dependent treatment based on the role of the V 1B receptor in stress regulation and the finding that stress is a trigger for relapse in alcoholics. A V 1B antagonist has shown favorable effects in rat models of alcohol dependence. A single-dose clinical study was conducted to assess the potential for pharmacokinetic or pharmacodynamic interaction between ABT-436 and alcohol.Methods: Twenty moderate alcohol drinkers each received the 4 possible combinations of a single 1,000 mg ABT-436 dose (or matching placebo) and a single 0.5 g/kg alcohol dose (or placebo for alcohol) in a double-blind, randomized, 4-period crossover study. Plasma ABT-436 and blood alcohol levels were measured to assess pharmacokinetic interactions. A computerized cognitive test battery (CDR System), Bond-Lader Visual Analog Scales scales, and a postural stability test were used to measure the effects of alcohol and the potential interaction with ABT-436. The pharmacologic effect of ABT-436 was assessed by measuring serum cortisol.Results: Neither ABT-436 nor alcohol affected the blood levels of the other. Alcohol reduced performance on 2 of 5 CDR System composite variables (power of attention, p = 0.002; quality of secondary episodic memory, p < 0.001), and decreased postural stability (p = 0.043). ABT-436 did not exacerbate those deleterious effects. ABT-436 reduced serum cortisol (p < 0.001), and alcohol did not significantly diminish this expected effect on the HPA axis.Conclusions: No pharmacokinetic or pharmacodynamic interaction between ABT-436 and alcohol was observed.

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Section: Methodsmentioning

confidence: 99%

Single‐Dose Interaction Study of the Arginine Vasopressin Type 1B Receptor Antagonist ABT‐436 and Alcohol in Moderate Alcohol Drinkers

Katz

Locke

Liu

et al. 2016

Alcoholism Clin & Exp Res

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“…In recent years, salting‐out assisted liquid–liquid extraction (SALLE) has received increasing attention and has been adapted as the sample preparation method in several high‐throughput LC–MS/MS bioanalyses because of its time‐saving procedure and low cost . Salting‐out, also called anti‐solvent crystallization, is a frequently used technology for separating proteins, based on electrolyte–nonelectrolyte interaction theory, which states that the solubility of nonelectrolytes in an aqueous solution is decreased when the concentration of electrolytes is increased.…”

Section: Introductionmentioning

confidence: 99%

High‐throughput salting‐out‐assisted liquid–liquid extraction for the simultaneous determination of atorvastatin, ortho‐hydroxyatorvastatin, and para‐hydroxyatorvastatin in human plasma using ultrafast liquid chromatography with tandem mass spectrometry

Yang

Zhou

et al. 2015

J of Separation Science

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A high-throughput, specific, and rapid liquid chromatography with tandem mass spectrometry method was established and validated for the simultaneous determination of atorvastatin and its two major metabolites, ortho-hydroxyatorvastatin and para-hydroxyatorvastatin, in human plasma. A simple salting-out-assisted liquid-liquid extraction using acetonitrile and a mass-spectrometry-friendly salt, ammonium acetate, was employed to extract the analytes from human plasma. A recovery of more than 81% for all analytes was achieved in 1 min extraction time. Chromatographic separation was performed on a Kinetex XB C18 column utilizing a gradient elution starting with a 60% of water solution (1% formic acid), followed by increasing percentages of acetonitrile. Analytes were detected on a tandem mass spectrometer equipped with an electrospray ionization source that was operated in the positive mode, using the transitions of m/z 559.3 → m/z 440.2 for atorvastatin, and m/z 575.3 → m/z 440.2 for both ortho- and para-hydroxyatorvastatin. Deuterium-labeled compounds were used as the internal standards. The method was validated over the concentration ranges of 0.0200-15.0 ng/mL for atorvastatin and ortho-hydroxyatorvastatin, and 0.0100-2.00 ng/mL for para-hydroxyatorvastatin with acceptable accuracy and precision. It was then successfully applied in a bioequivalence study of atorvastatin.

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“…In another innovation, miniaturization of salting-out technique was successfully employed for significant cost saving for analyzing sulfonamides (Liu et al, 2010). The ruggedness and reproducibility of the salting-out sample preparation step has been confirmed with incurred sample analysis for a new investigational agent (Zhang et al, 2009a(Zhang et al, , 2009b.…”

mentioning

confidence: 99%

“…Hence, this technique would not only provide similar efficiency to protein precipitation, but would also provide an avenue to analyze both hydrophilic and hydrophobic moieties with cleaner background noise and improved sensitivity. Recently, the adaptation of this technique has been taken to the next level by automation for increased throughput and analysis of scores of compounds (Wang et al, 2006;Zhang et al, 2006Zhang et al, , 2007Zhang et al, , 2009aZhang et al, , 2009bKim et al, 2007;Wu et al, 2008).…”

mentioning

confidence: 99%

The re‐emergence of salting‐out association with acetonitrile for modern day bioanalysis

Srinivas¹

2013

Biomedical Chromatography

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High-Throughput Salting-Out Assisted Liquid/Liquid Extraction and Ultrafast LC for Same-Day Delivery of First-in-Human Bioanalytical Data

Cited by 14 publications

References 13 publications

Single‐Dose Interaction Study of the Arginine Vasopressin Type 1B Receptor Antagonist ABT‐436 and Alcohol in Moderate Alcohol Drinkers

Single‐Dose Interaction Study of the Arginine Vasopressin Type 1B Receptor Antagonist ABT‐436 and Alcohol in Moderate Alcohol Drinkers

High‐throughput salting‐out‐assisted liquid–liquid extraction for the simultaneous determination of atorvastatin, ortho‐hydroxyatorvastatin, and para‐hydroxyatorvastatin in human plasma using ultrafast liquid chromatography with tandem mass spectrometry

The re‐emergence of salting‐out association with acetonitrile for modern day bioanalysis

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