High-throughput RNA sequencing reveals the anti-inflammatory mechanism of baicalin on Propionibacterium acnes-induced acne in rabbits

Yang, Xingzhe; Huang, Guangrui; You, Leiming; Sheng, Honghao; Sun, Yuxiu; Feng, Yuyin; Lu, Qing‐Yi; Xu, Anlong

doi:10.1016/j.jtcms.2019.09.001

Cited by 1 publication

(1 citation statement)

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“… 2017 ). Baicalin can inhibit multiple inflammatory pathways in the process of acne, such as TNF signalling pathway and pathway of Staphylococcus aureus infections (Yang X 2019 ). Some active chemical components of QCF also play an antibacterial effect.…”

Section: Discussionmentioning

confidence: 99%

Investigating material basis and molecular mechanism of Qing Cuo formula in the treatment of acne based on animal experiments, UPLC-LTQ-Orbitrap-MS and network pharmacology

Cao

Liang

Wang

et al. 2023

Pharmaceutical Biology

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Context Qing Cuo Formula (QCF) is a traditional Chinese medicine for treating acne, but its active compounds and molecular mechanisms are unclear. Objective To investigate the material basis and molecular mechanism of QCF. Materials and methods In vivo experiments were conducted on 60 male golden hamsters with damp-heat acne, with a blank group, a spironolactone group and 3 QCF administration groups (given high, medium and low doses) over a 30-day period. Serum androgen and inflammatory cytokine levels were tested by ELISA. In vitro , chemical compositions of QCF were investigated by UPLC-LTQ-Orbitrap-MS. Network pharmacology approaches were used to analyse the protein–protein interaction (PPI) network and QCF active compounds-intersection targets-acne network. GO enrichment and KEGG pathway analysis was conducted subsequently. Results Low-dose QCF group (11.4 g/kg/day) showed significantly reduced levels of serum T (4.94 ± 0.36; 5.51 ± 0.36 ng/mL), DHT (6.67 ± 0.61; 8.09 ± 0.59 nmol/L), E2 (209.01 ± 20.92; 237.08 ± 13.94 pg/mL), IL-1α (36.84 ± 3.23; 44.07 ± 4.00 pg/mL) and FFA (128.32 ± 10.94; 148.00 ± 12.12 µmol/L) compared to the blank group ( p < 0.05). In vitro experiments identified 75 compounds in QCF decoction, with 27 active compounds absorbed in serum. Network pharmacology identified 6 active components connecting 17 targets. GO enrichment and KEGG pathway analysis indicated that QCF’s anti-acne targets mainly regulate extracellular matrix function, inflammatory processes, immune response and endocrine function. Conclusions This study provides evidence of the molecular mechanism and material basis of QCF in treating androgen-related damp-heat acne, paving the way for further research on its potential in treating other conditions related to damp-heat constitution.

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Section: Discussionmentioning

confidence: 99%