High-throughput residual white blood cell counter enabled by microfluidic cell enrichment and reagent-containing patch integration

Kim, Byeongyeon; Shin, Suyeon; Lee, Yujin; Um, Changyong; You, Dongwon; Yun, Hong Sik

doi:10.1016/j.snb.2018.12.069

Cited by 8 publications

(17 citation statements)

References 32 publications

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“…[92][93][94] In addition to WBC counting, these smart devices can also be used to detect and analyze certain chemicals in the blood so as to realize the low-cost and convenient medical testing of, for example, blood cholesterol [95], blood ketone [96], blood sugar [97], blood lipid [98], etc. Table 3 [82,87,88,91,[99][100][101][102][103] shows some of the latest separation and counting methods for WBCs.…”

Section: Recent Development Of Leucocyte Counting At Pocmentioning

confidence: 99%

White blood cell counting at point‐of‐care testing: A review

Luo

Chen

2020

Electrophoresis

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White blood cells, which are also called leukocytes, are found in the immune system that are involved in protecting the body against infections and foreign invaders. Conventional methods of leukocyte analysis provide valuable and accurate information to medical specialists. Analyzing and diagnosing of a disease requires a combination of multiple biomarkers, in some cases, however, such as personal health care, this will occupy some medical resources and causes unnecessary consumption. Traditional method (such as flow cytometer) for WBC counting is time and labor consuming. Compared to gold standard (flow‐based fraction/micropore filtration) or improved filtration methods for WBC counting, this is still a lengthy and time consuming process and can lead to membrane fouling due to the rapid accumulation of biological materials. Therefore, the analysis of WBC counts requires more compact and efficient equipment. The microfluidic technologies, powered by different field (force, thermal, acoustic, optical, magnetic) and other methods for leukocyte counting and analysis, are much cost‐efficient and can be used in in‐home or in resource‐limited areas to achieve Point‐of‐Care (POC). In this review, we highlight the mainstream devices that have been commercialized and extensively employed for patients for WBC counting, Next, we present some recent development with regards to leucocyte counting (mainly microfluidic technologies) and comment on their relative merits. We aim to focus and discuss the possibility of achieving POC and help researchers to tackle individual challenges accordingly. Finally, we offer some technologies in addition to previous detection devices, such as image recognition technology and cloud computing, which we believe have great potential to further promote real‐time detection and improve medical diagnosis.

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Section: Recent Development Of Leucocyte Counting At Pocmentioning

confidence: 99%

White blood cell counting at point‐of‐care testing: A review

Luo

Chen

2020

Electrophoresis

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“…A microuidic comparator was fabricated by standard photolithography and polydimethylsiloxane (PDMS) replica molding, as reported elsewhere. [2][3][4][5][6][7][8][9] Briey, the channel patterns of 57.5 mm height were photolithographically dened in a SU-8 photoresist lm, and then transferred to PDMS by thermal curing of a mixture of PDMS and its curing agent at a 10 : 1 (wt/ wt) mass ratio on the channel patterns. The nal PDMS device has a single outlet and a 2 mm-wide junction channel connected to two inlet channels, allowing the direct comparison of the ow rates of two uid streams injected into the device inlets.…”

Section: Device Fabricationmentioning

confidence: 99%

“…A miniaturized uorescence microscope was fabricated by simply assembling a laser diode (488 nm; DTR Laser, United States), a longpass lter with a cut-on wavelength of 515 nm (Omega Optical, Inc., United States), a 2Â objective lens (Edmund Optics, Inc., United States), and a CMOS sensor (FLIR, Inc., Canada) in a 3D-printed housing, as described before. 9,21 The eld-of-view of the miniaturized microscope was 1.446 mm Â 0.579 mm, which is large enough to capture the region of interrogation, the outlet channel region (400 mm in width) of the sheathless focuser.…”

Section: Device Fabricationmentioning

confidence: 99%

“…Continuous and programmable ow-rate generation is important for the reliable operation of microuidic devices, the performance of which oen relies on ow conditions. [1][2][3][4][5][6][7][8][9] For example, most microuidic cell separators and focusers have an optimum ow-rate range at which cells can have enough time to reach an equilibrium separation or focusing position by external forces [2][3][4] or intrinsic ow elds. [5][6][7] The signal intensity and counting sensitivity of microuidic cell counters can vary signicantly, depending on ow rates.…”

Section: Introductionmentioning

confidence: 99%

“…[5][6][7] The signal intensity and counting sensitivity of microuidic cell counters can vary signicantly, depending on ow rates. 8,9 For such microuidic applications, syringe pumps have been extensively used with high precision and smooth operation, despite bulky equipment size and high cost. In addition to commercially-available syringe pumps, a 3D-printed, opensource syringe pump has recently been developed, 10 creating low cost, highly customizable scientic equipment, and thus making available the essential uidic equipment even for nonexpert users to conduct microuidic applications.…”

Section: Introductionmentioning

confidence: 99%

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An open-source programmable smart pipette for portable cell separation and counting

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Microfluidic Label‐Free Hydrodynamic Separation of Blood Cells: Recent Developments and Future Perspectives

Lee

Kim

Yang

2023

Adv Materials Technologies

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The biophysical characteristics and counts of blood cells provide useful information regarding pathological conditions. Therefore, the separation of blood components is a crucial task in biology, clinical diagnosis, therapeutics, and personalized medicine. Recently, microfluidics has gained significant interest as an effective technology for separating target cells from heterogeneous cell populations. Within cellular-scale microchannels, cells of interest are separated based on intrinsic properties, such as size, shape, and deformability, followed by further downstream or off-chip analysis. In this review, microfluidic-based hydrodynamic cell-separation technologies used in label-free approaches by categorizing them according to their key working principles are discussed: i) flow fractionation, ii) deterministic lateral displacement, iii) hydrophoresis, iv) inertial migration, and v) microfiltration. An overview of the major separation mechanisms is provided, and the relative performances and features of these technologies are thoroughly discussed. In addition, future perspectives regarding microfluidic system commercialization and standardization are briefly provided for their widespread use and applications.

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High-throughput residual white blood cell counter enabled by microfluidic cell enrichment and reagent-containing patch integration

Cited by 8 publications

References 32 publications

White blood cell counting at point‐of‐care testing: A review

White blood cell counting at point‐of‐care testing: A review

An open-source programmable smart pipette for portable cell separation and counting

Microfluidic Label‐Free Hydrodynamic Separation of Blood Cells: Recent Developments and Future Perspectives

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