High throughput rapid amplicon sequencing for multilocus sequence typing of M. ovipneumoniae using DNA obtained from clinical samples
Isaac Framst,
Rebecca M. Wolking,
Justin Schonfeld
et al.
Abstract:Background
Spillover events of Mycoplasma ovipneumoniae have devastating effects on wild bighorn sheep populations. Multilocus sequence typing (MLST), a common method for tracking bacterial lineages, is used to monitor spillover events and the spread of M. ovipneumoniae between populations. Most work involving M. ovipneumoniae typing has used Sanger sequencing, however, this technology is time consuming, expensive, and is not well suited to efficient batch sample processing. Our study aimed to develop and val… Show more
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