High-throughput quantitative method for assessing coaggregation among oral bacterial species

Levin-Sparenberg, Elizabeth; Shin, Jae Min; Hastings, E.M.; Freeland, Melissa; Segaloff, Hannah E; Rickard, Alexander H.; Foxman, Betsy

doi:10.1111/lam.12622

Cited by 10 publications

(8 citation statements)

References 28 publications

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“…Coaggregation describes the specific interaction of pairs of oral bacteria via cognate binding. Intergeneric microbial coaggregation clearly contributes to the characteristics of the complex microbial ecology of biofilms established in the multiple habitats of the oral cavity . Many studies of bacterial coaggregation in oral biofilms have focused on the ability of ‘climax pathogenic’ species, such as P. gingivalis , to bind to early or bridging colonizers in this complex microbial ecology.…”

Section: Characteristics Of the Microbiome In The Oral Cavity And Othmentioning

confidence: 99%

The periodontal war: microbes and immunity

Ebersole¹,

Dawson²,

Emecen‐Huja³

et al. 2017

Periodontology 2000

153

120

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Maintenance of periodontal health or transition to a periodontal lesion reflects the continuous and ongoing battle between the vast microbial ecology in the oral cavity and the array of resident and emigrating inflammatory/immune cells in the periodontium. This war clearly signifies many 'battlefronts' representing the interface of the mucosal-surface cells with the dynamic biofilms composed of commensal and potential pathogenic species, as well as more recent knowledge demonstrating active invasion of cells and tissues of the periodontium leading to skirmishes in connective tissue, the locality of bone and even in the local vasculature. Research in the discipline has uncovered a concerted effort of the microbiome, using an array of survival strategies, to interact with other bacteria and host cells. These strategies aid in colonization by 'ambushing, infiltrating and outflanking' host cells and molecules, responding to local environmental changes (including booby traps for host biomolecules), communicating within and between genera and species that provide MASINT (Measurement and Signature Intelligence) to enhance sustained survival, sabotage the host inflammatory and immune responses and by potentially adopting a 'Fabian strategy' with a war of attrition and resulting disease manifestations. Additionally, much has been learned regarding the ever-increasing complexity of the host-response armamentarium at both cellular and molecular levels that is addressed in this review. Knowledge regarding how these systems fully interact requires both new laboratory and clinical tools, as well as sophisticated modeling of the networks that help maintain homeostasis and are dysregulated in disease. Finally, the triggers resulting in a 'coup de main' by the microbiome (exacerbation of disease) and the characteristics of susceptible hosts that can result in 'pyrrhic victories' with collateral damage to host tissues, the hallmark of periodontitis, remains unclear. While much has been learned, substantial gaps in our understanding of the 'parameters of this war' remain elusive toward fulfilling the Sun Tzu adage: 'If you know the enemy and know yourself, you need not fear the result of a hundred battles.'

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Section: Characteristics Of the Microbiome In The Oral Cavity And Othmentioning

confidence: 99%

The periodontal war: microbes and immunity

Ebersole¹,

Dawson²,

Emecen‐Huja³

et al. 2017

Periodontology 2000

153

120

View full text Add to dashboard Cite

show abstract

“…A wide range of co‐culture strategies has been developed to facilitate the characterization of interspecies relationships, largely focusing on metabolic compatibility and coaggregation of species that form oral biofilms and plaque (Levin‐Sparenberg et al, 2016; Ochiai et al, 1993; Postollec et al, 2006). Coaggregation in suspension and co‐adhesion on surfaces between bacterial species appear to share common pathways (Kolenbrander & Andersen, 2000).…”

Section: Introductionmentioning

confidence: 99%

No man's land: Species‐specific formation of exclusion zones bordering Actinomyces graevenitzii microcolonies in nanoliter cultures

Jalali

Ellett

Balani³

et al. 2021

MicrobiologyOpen

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To survive within complex environmental niches, including the human host, bacteria have evolved intricate interspecies communities driven by competition for limited nutrients, cooperation via complementary metabolic proficiencies, and establishment of homeostatic relationships with the host immune system. The study of such complex, interdependent relationships is often hampered by the challenges of culturing many bacterial strains in research settings and the limited set of tools available for studying the dynamic behavior of multiple bacterial species at the microscale. Here, we utilize a microfluidic‐based co‐culture system and time‐lapse imaging to characterize dynamic interactions between Streptococcus species, Staphylococcus aureus, and Actinomyces species. Co‐culture of Streptococcus cristatus or S. salivarius in nanoliter compartments with Actinomyces graevenitzii revealed localized exclusion of Streptococcus and Staphylococcus from media immediately surrounding A. graevenitzii microcolonies. This community structure did not occur with S. mitis or S. oralis strains or in co‐cultures containing other Actinomycetaceae species such as S. odontolyticus or A. naeslundii. Moreover, fewer neutrophils were attracted to compartments containing both A. graevenitzii and Staphylococcus aureus than to an equal number of either species alone, suggesting a possible survival benefit together during immune responses.

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“…Although this is not a true aggregate size distribution, owing to the fact the aggregates are 3-dimensional entities, it nevertheless allows one to compare aggregates at different stages during growth and also to compare aggregates between different strains. Combining this type of analysis with high throughput methods, like those used to assess coaggregation in aggregates of oral bacteria (85), will allow for a better understanding of aggregation in P. aeruginosa and in other strains that are clinically and industrially relevant.…”

Section: Discussionmentioning

confidence: 99%

Distinct types of multicellular aggregates in Pseudomonas aeruginosa liquid cultures

Melaugh

Martinez

Baker

et al. 2022

Preprint

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Pseudomonas aeruginosa forms suspended multicellular aggregates when cultured in liquid media. Such aggregates may be important in disease, and/or as a pathway to biofilm formation. The polysaccharide Psl and extracellular DNA (eDNA) have both been implicated in aggregation, but previous results depend strongly on the experimental conditions. Here we develop a quantitative microscopy-based method for assessing changes in the size distribution of suspended aggregates over time in growing cultures. For exponentially growing cultures of P. aeruginosa PAO1, we find that aggregation is mediated by cell-associated Psl, rather than by either eDNA or secreted Psl. These aggregates arise de novo within the culture via a growth process that involves both collisions and clonal growth. They are "non-cheatable" since Psl non-producing cells do not aggregate with producers. In contrast, we find that stationary phase (overnight) cultures contain a different type of multicelullar aggregate, in which both eDNA and Psl mediate cohesion. Our findings suggest that the physical and biological properties of multicellular aggregates may be very different in early-stage vs late-stage bacterial cultures.

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High-throughput quantitative method for assessing coaggregation among oral bacterial species

Cited by 10 publications

References 28 publications

The periodontal war: microbes and immunity

The periodontal war: microbes and immunity

No man's land: Species‐specific formation of exclusion zones bordering Actinomyces graevenitzii microcolonies in nanoliter cultures

Distinct types of multicellular aggregates in Pseudomonas aeruginosa liquid cultures

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