High-Throughput Quantitative Measurement of Bacterial Attachment Kinetics on Seconds Time Scale

Shteindel, Nimrod; Yankelev, Dimitry; Gerchman, Yoram

doi:10.1007/s00248-018-1254-5

Cited by 10 publications

(19 citation statements)

References 30 publications

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“…The effect of amoeba population density on P. aeruginosa congregation was measured using a kinetic assay in a high-throughput setting ( Fig. 3a ) ( 33 ). Adhesion was found to be correlative to amoeba population density ( Fig.…”

Section: Resultsmentioning

confidence: 99%

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Pseudomonas aeruginosa Mobbing-Like Behavior against Acanthamoeba castellanii Bacterivore and Its Rapid Control by Quorum Sensing and Environmental Cues

Shteindel

Gerchman

2021

Microbiol Spectr

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Section: Resultsmentioning

confidence: 99%

“…Bacterial adhesion to amoebae was quantified using a kinetic assay in a 96-well plate ( 33 ) ( Fig. 3 ).…”

Section: Methodsmentioning

confidence: 99%

Pseudomonas aeruginosa Mobbing-Like Behavior against Acanthamoeba castellanii Bacterivore and Its Rapid Control by Quorum Sensing and Environmental Cues

Shteindel

Gerchman

2021

Microbiol Spectr

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“…Khachikyan et al (2019) used a microfluidic mass sensor to directly measure the cellular dry weight and carbon (C) mass of environmentally relevant prokaryotic microorganisms for use in estimating the contribution of these organisms to ecosystem function and global microbial biomass. Using a microtiter plate‐based assay, Shteindel, Yankelev, and Gerchman (2019) developed a reproducible technique to measure microbial attachment kinetics during the early stages of biofilm formation.…”

Section: Methodsmentioning

confidence: 99%

Substratum‐associated microbiota

Furey

Lee

Clemans

2020

Water Environment Research

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Highlights of new, interesting, and emerging research findings on substratum-associated microbiota covered from a survey of 2019 literature from primarily freshwaters provide insight into research trends of interest to the Water Environment Federation and others interested in benthic, aquatic environments. Coverage of topics on bottom-associated or attached algae and cyanobacteria, though not comprehensive, includes new methods, taxa new-to-science, nutrient dynamics, auto-and heterotrophic interactions, grazers, bioassessment, herbicides and other pollutants, metal contaminants, and nuisance, and bloom-forming and harmful algae. Coverage of bacteria, also not comprehensive, focuses on the ecology of benthic biofilms and microbial communities, along with the ecology of microbes like Caulobacter crescentus, Rhodobacter, and other freshwater microbial species. Bacterial topics covered also include metagenomics and metatranscriptomics, toxins and pollutants, bacterial pathogens and bacteriophages, and bacterial physiology. Readers may use this literature review to learn about or renew their interest in the recent advances and discoveries regarding substratum-associated microbiota. © 2020 Water Environment Federation • Practitioner points • This review of literature from 2019 on substratum-associated microbiota presents highlights of findings on algae, cyanobacteria, and bacteria from primarily freshwaters. • Coverage of algae and cyanobacteria includes findings on new methods, taxa new to science, nutrient dynamics, auto-and heterotrophic interactions, grazers, bioassessment, herbicides and other pollutants, metal contaminants, and nuisance, bloomforming and harmful algae. • Coverage of bacteria includes findings on ecology of benthic biofilms and microbial communities, the ecology of microbes, metagenomics and metatranscriptomics, toxins and pollutants, bacterial pathogens and bacteriophages, and bacterial physiology. • Highlights of new, noteworthy and emerging topics build on those from 2018 and will be of relevance to the Water Environment Federation and others interested in benthic, aquatic environments

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“…Bacterial adhesion to amoebae was quantified using a kinetic assay in 96 well plate format [12] (figure 2a of this work). Amoeba culture in TBSS was diluted to 8X10 4…”

Section: Effect Of Amoeba Population Density On P Aeruginosa Attachmmentioning

confidence: 99%

Bacterial mobbing behavior: coordinated communal attack ofPseudomonas aeruginosaon a protozoan predator

Shteindel

Gerchman

2020

Preprint

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Mobbing, a group attack of prey on predator, is a strategy enacted by many animal species. Here we report bacterial mobbing carried out by the bacterium Pseudomonas aeruginosa towards Acanthamoeba castellanii, a common bacterivore. This behavior consists of bacterial taxis towards the amoebae, adhesion en masse to amoebae cells, and eventual killing of the amoebae. Mobbing behavior transpires in second’s timescale and responds to predator population density. A mutant defective in the production of a specific quorum sensing signal displays reduced adhesion to amoeba cells. This deficiency ameliorated by external addition of the missing signal molecule. The same quorum sensing mutant also expresses long term deficiency in its ability to cause amoeba death and shows higher susceptibility to predation, highlighting the importance of group coordination to mobbing and predation avoidance. These findings portray bacterial mobbing as a regulated and dynamic group behavior.

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High-Throughput Quantitative Measurement of Bacterial Attachment Kinetics on Seconds Time Scale

Cited by 10 publications

References 30 publications

Pseudomonas aeruginosa Mobbing-Like Behavior against Acanthamoeba castellanii Bacterivore and Its Rapid Control by Quorum Sensing and Environmental Cues

Pseudomonas aeruginosa Mobbing-Like Behavior against Acanthamoeba castellanii Bacterivore and Its Rapid Control by Quorum Sensing and Environmental Cues

Substratum‐associated microbiota

Bacterial mobbing behavior: coordinated communal attack ofPseudomonas aeruginosaon a protozoan predator

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