DOI: 10.1155/2013/905704
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Abstract: A highly specific, sensitive, and rapid method, to quantify gemifloxacin in human urine using HPLC coupled to the triple quadrupole mass spectrometer system, was developed and validated. Gemifloxacin and ofloxacin (internal standard) were rapidly extracted from urine samples without any tedious pretreatment procedure. Urine samples were filtered through a Millex-GP, 0.22 m syringe filter. Optimal chromatographic separation of the analytes was achieved on Zorbax SB-C 18 (30 mm × 2 mm i.d., 3.5 m maintained at a…

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