“…Consequently, it was concluded that changes in the expression level of detected proteins are not helpful for identifying or monitoring the processes of cellular differentiation of both P19 and P19C6 cells. Compared with the results of proteomebased analysis, the high potential of the glycome-based approach is clear because our results revealed for the first time that 28% of glycoforms (19 N-glycans of 67 total N-glycans; peak numbers 13,20,21,22,25,28,29,32,34,37,38,39,40,41,47,48,54,60, and 63) were increased, and 10% (7 of 67) were decreased during P19CL6 cell differentiation to car- 1) and sialyl-Lewis X tetrasaccharide moieties in major N-glycans as shown in Fig. 3C, whereas monofucosylation occurred specifically in the above 31 glycoforms at the GlcNAc residue involved in core chitobiose moiety after differentiation, suggesting that loss of cell adhesion through the interaction with selectins may be key to the differentiation of P19CL6 cells toward cardiomyocytes.…”