High-Throughput Protein and DNA Analysis Based on Microfluidic On-Chip Electrophoresis

Abstract

M icrofluidic technology applied to on-chip electrophoresis provides high-throughput DNA or protein analysis in an automated, unattended mode, which is currently not possible with any other technology. The 5100 Automated Lab-on-a-Chip Platform automates all the required experimental steps, including sample loading from multiple sample plates, electrophoresis, staining/destaining, and detection. The analysis of the digital data is completely automated as well and the results together with all other information, such as sample names, are directly fed into a database. The article describes in detail the design of the microfluidic system, including instrumentation, chips, DNA and protein assays, as well as the structure and the main features of the software.

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