High‐Throughput Phenotyping and Improvements in Breeding Cassava for Increased Carotenoids in the Roots

Belalcázar, John; Dufour, Dominique; Andersson, Meike S.; Pizarro, Mónica; Luna, Jorge; Londoño, Luis Alfonso Correa; Morante, Nelsón; Jaramillo, Angélica; Pino, Lizbeth; López-Lavalle, Luis Agusto Becerra; Davrieux, Fabrice; Talsma, Elise F.; Ceballos, Hernán

doi:10.2135/cropsci2015.11.0701

Cited by 42 publications

(45 citation statements)

References 35 publications

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“…An improved RCRS scheme has been recently described [85]. In this scheme, seedling plants (F1 stage) are grown only for 6 months.…”

Section: Evolution and Improvements Of Breeding Methods To Increase Cmentioning

confidence: 99%

“…After 5 years of harvest and database building, NIR spectroscopy coupled with LOCAL regression led to accurate and robust calibrations for breeding programs aiming at increasing carotenoids content in fresh cassava roots [85]. These results offer immense prospects for many cassava-breeding projects in the world; NIRS overtakes the bottleneck of conventional carotenoids quantification methods.…”

Section: Evolution and Improvements Of Quantifying Protocols For Caromentioning

confidence: 99%

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Carotenoids in Cassava Roots

Ceballos¹,

Davrieux²,

Talsma³

et al. 2017

Carotenoids

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Vitamin A deficiency (VAD) is a preventable tragedy that affects millions of people, particularly in sub-Saharan Africa. A large proportion of these people rely on diets based on cassava as a source of calories. During the last two decades, significant efforts have been made to identify sources of germplasm with high pro-vitamin A carotenoids (pVAC) and then use them to develop cultivars with a nutritional goal of 15 μg g −1 of β-carotene (fresh weight basis) and good agronomic performance. The protocols for sampling roots and quantifying carotenoids have been improved. Recently, NIR predictions began to be used. Retention of carotenoids after different root processing methods has been measured. Bioavailability studies suggest high conversion rates. Genetic modification has also been achieved with mixed results. Carotenogenesis genes have been characterized and their activity in roots measured.

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“…An improved RCRS scheme has been recently described [85]. In this scheme, seedling plants (F1 stage) are grown only for 6 months.…”

Section: Evolution and Improvements Of Breeding Methods To Increase Cmentioning

confidence: 99%

Section: Evolution and Improvements Of Quantifying Protocols For Caromentioning

confidence: 99%

Carotenoids in Cassava Roots

Ceballos¹,

Davrieux²,

Talsma³

et al. 2017

Carotenoids

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“…However, they tend to overestimate carotenoid content when compared to HPLC due to other compounds also detected [21,22]. Near-infrared spectrometry (NIRS) has proven a costand time-efficient method for high-throughput screening for carotenoid content in sweet potato [23], banana [24,25], and cassava as well as for other important breeding traits such as dry matter content (DMC) and cyanogenic potential [26,27,28]. New portable devices such as iCheck and portable NIRS are being evaluated and show promise for use in the field when transport of fresh cassava roots to the nearest laboratory is a challenge [29].…”

Section: Provitamin a Carotenoidsmentioning

confidence: 99%

Progress update: Crop development of biofortified staple food crops under HarvestPlus

Andersson¹,

Saltzman²,

Virk³

et al. 2017

AJFAND

125

130

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“…When TCC is expressed to dry-matter content (dry weight), it steadily increases at 8-12 months after planting (Ortiz et al 2011). Despite what has been posited by Ortiz et al (2011), where the relationship between dry matter and TCC in 26 genotypes was analyzed, the latest evidence, after analyzing more than 4000 cassava genotypes, indicates that there is no correlation between dry-matter content and the level of carotenoids in the roots, at least for market varieties whose dry-matter content ranged between 25 and 45% (Ceballos et al 2013;Belalcazar et al 2016).…”

Section: Discussionmentioning

confidence: 85%

Molecular analysis of the expression of a crtB transgene and the endogenous psy2-y 1 and psy2-y 2 genes of cassava and their effect on root carotenoid content

Chavarriaga-Aguirre

Prías

López³

et al. 2017

Transgenic Res

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A conventional breeding program was established to transfer the bacterial phytoene synthase transgene-crtB-from a transgenic, white-rooted cassava to yellow-rooted cassava plants carrying the endogenous phytoene synthase alleles named psy2-y and/or psy2-y. Combining endogenous phytoene synthase enzymes (PSYs) with CRTB in a single cassava plant would allow the molecular dissection of individual allele contributions to carotenoid synthesis and/or accumulation in cassava roots. The simultaneous expression of the crtB transgene and psy2-y in individuals planted in the field coincided with higher total, HPLC-quantified carotenoid content in roots, although the variability among replications (plants) precluded the detection of statistically significant differences. Nevertheless, the highest total carotenoid content in roots within a family coincided with one individual of the F1 progeny carrying both psy2-y and crtB genes. The results also indicated the presence of at least one more key gene-different from psy or crtB-which too is necessary for the synthesis and/or accumulation of Pro-Vitamin A carotenoids in cassava roots.

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High‐Throughput Phenotyping and Improvements in Breeding Cassava for Increased Carotenoids in the Roots

Abstract: Past research developed reliable equations to base selections for high b-carotene on nearinfrared spectroscopy (NIR) predictions (100 genotypes d −1

Cited by 42 publications

References 35 publications

Carotenoids in Cassava Roots

Carotenoids in Cassava Roots

Progress update: Crop development of biofortified staple food crops under HarvestPlus

Molecular analysis of the expression of a crtB transgene and the endogenous psy2-y 1 and psy2-y 2 genes of cassava and their effect on root carotenoid content

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