High throughput optical modulation biosensing for highly sensitive and rapid detection of biomarkers

Burg, Shmuel; Roth, Shira; Cohen, Meir; Avivi-Mintz, Shira; Margulis, Michael; Rohana, Hanan; Peretz, Avi; Danielli, Amos

doi:10.1016/j.talanta.2022.123624

Cited by 4 publications

(2 citation statements)

References 26 publications

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“…For this purpose, various methods have been developed. 5,6 In order to improve the detection throughput, multichannel detection is the most commonly used method. There are two kinds of strategies: electrode array and multichannel on single-electrode.…”

Section: ■ Introductionmentioning

confidence: 99%

“…However, with the growing demands for detection, PEC analysis methods are required to have higher throughput and accuracy. For this purpose, various methods have been developed. , In order to improve the detection throughput, multichannel detection is the most commonly used method. There are two kinds of strategies: electrode array and multichannel on single-electrode. , For the former, the array consists of multiple independent electrodes, and each electrode can be used to fabricate a biosensor for the analysis of one object, which enables the analysis of multiple samples but usually makes the instrumentation more complex.…”

Section: Introductionmentioning

confidence: 99%

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Magnetically Controlled and Addressable Photoelectrochemical Sensor Array with Self-Calibration for the Label-Free Detection of Amyloid β-Proteins

Wang,

Zhang,

Zhang

et al. 2023

Anal. Chem.

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A label-free addressable photoelectric immunosensor array was designed for the detection of amyloid β-proteins based on magnetic separation and self-calibration strategies. In this paper, Na 2 Ti 6 O 13 with a flower-like morphology was prepared by the hydrothermal method; after continuously combining Fe 3 O 4 and CdS, it was endowed with magnetism and better photoelectric activity. Subsequently, a series of reactions occurred in the solution, and the magnetic separation method was used to enrich the target. On the other hand, the ITO glass was separated into eight sites (2 × 4) using magnets, and a light shield was utilized to prevent light exposure, resulting in addressable and continuous detection. After the uniform preparation of magnetic photoelectric materials and precise control of testing conditions, the relative errors among different sites have been effectively reduced. Moreover, incorporating a self-calibration strategy has allowed the sensor array to achieve greater accuracy. The proposed photoelectrochemical biosensor exhibits a good relationship with amyloid β-protein ranging from 0.01 to 100 ng mL −1 with a limit of detection of 1.1 pg mL −1 and exhibits excellent specificity, reproducibility, and stability.

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Section: ■ Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Magnetically Controlled and Addressable Photoelectrochemical Sensor Array with Self-Calibration for the Label-Free Detection of Amyloid β-Proteins

Wang,

Zhang,

Zhang

et al. 2023

Anal. Chem.

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Trends and challenges in electroanalytical biosensing methodologies for infectious viral diseases

Panicker,

Kummari,

Keerthanaa

et al. 2024

Bioelectrochemistry

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Highly sensitive extraction-free saliva-based molecular assay for rapid diagnosis of SARS-CoV-2

Margulis,

Rohana,

Erster

et al. 2024

J Clin Microbiol

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The COVID-19 pandemic highlighted the necessity of fast, sensitive, and efficient methods to test large populations for respiratory viruses. The “gold standard” molecular assays for detecting respiratory viruses, such as quantitative polymerase chain reaction (qPCR) and reverse transcription qPCR (RT-qPCR), rely on invasive swab samples and require time-consuming and labor-intensive extraction processes. Moreover, the turnaround time for RT-qPCR-based assays is too lengthy for rapid screening. Extraction-free saliva-based methods provide a non-invasive sampling process with a fast turnaround time and are suitable for high-throughput applications. However, when used with a standard RT-qPCR system, the absence of extraction significantly reduces the assays’ sensitivity. Here, using a novel optical modulation biosensing (OMB) platform, we developed a rapid and highly sensitive extraction-free saliva-based molecular assay. We blindly tested 364 paired nasopharyngeal swabs and saliva samples from suspected SARS-CoV-2 cases in Israel. Compared with the gold standard swab-based RT-qPCR assay, the sensitivity of the extraction-free saliva-based OMB assay is 90.7%, much higher than the sensitivity of extraction-free saliva-based RT-qPCR assay (77.8%) with similar specificity (95.3% and 97.6%, respectively). Moreover, out of 12 samples identified by the OMB-based assay as positive, 8 samples were collected from hospitalized patients in a COVID-19 ward and were verified to be SARS-CoV-2-positive upon admission, indicating that the actual clinical sensitivity and specificity of the OMB assay are higher. Considering its user-friendly saliva-based protocol, short and cost-effective extraction-free process, and high clinical accuracy, the OMB-based molecular assay is very suitable for high-throughput testing of large populations for respiratory viruses. IMPORTANCE Three years after the SARS-CoV-2 outbreak, there are no molecular tests that combine low-cost and straightforward sample preparation, effective sample handling, minimal reagent and disposable requirements, high sensitivity, and high throughput required for mass screening. Existing rapid molecular techniques typically sacrifice certain requirements to meet others. Yet, localized outbreaks of novel viral diseases happen daily in different parts of the world. In this context, respiratory diseases are of specific importance, as they are frequently airborne and highly contagious, with the potential for a rapid global spread. The widely accepted opinion is that another pandemic is just a question of time. To ensure that the containment efforts for the upcoming “disease X” are successful, introducing rapid, high-throughput, and highly sensitive diagnostic methods for detecting and identifying pathogens is critical. A few months into the pandemic, saliva was suggested as a diagnostic matrix for SARS-CoV-2 detection. The collection of saliva does not require swabs and is minimally invasive. In particular, extraction-free saliva-based assays require fewer reagents and disposables, and therefore are faster and cheaper, offering an appealing alternative for low-income countries. Unfortunately, current extraction-free saliva-based detection methods, such as direct RT-qPCR or isothermal amplification, have either low sensitivity or low throughput. Therefore, we believe that the presented highly sensitive ht-OMBi platform and the extraction-free saliva-based molecular assay can become an essential tool in the infectious disease monitoring toolbox .

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High throughput optical modulation biosensing for highly sensitive and rapid detection of biomarkers

Cited by 4 publications

References 26 publications

Magnetically Controlled and Addressable Photoelectrochemical Sensor Array with Self-Calibration for the Label-Free Detection of Amyloid β-Proteins

Magnetically Controlled and Addressable Photoelectrochemical Sensor Array with Self-Calibration for the Label-Free Detection of Amyloid β-Proteins

Trends and challenges in electroanalytical biosensing methodologies for infectious viral diseases

Highly sensitive extraction-free saliva-based molecular assay for rapid diagnosis of SARS-CoV-2

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