High throughput micropatterning of interspersed cell arrays using capillary assembly

Delapierre, François-Damien; Mottet, Guillaume; Taniga, Vélan; Boisselier, Julie; Viovy, Jean‐Louis; Malaquin, Laurent

doi:10.1088/1758-5090/aa5852

Cited by 11 publications

(9 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Capillary assembly has also been used to improve inking of molecules onto PDMS stamps resulting in much improved prints [ 68 , 69 ], using specific antibody coated at the surface of the particles and targeting cell membrane proteins. Delapierre et al were able to capture and place specific types of cells onto the stamp [ 70 ]. Alternate grooves at 90° angle to each others allowed the alternate capture of particles coated with different antibodies capable of attaching two different cell types on the stamp.…”

Section: Techniques and Methodsmentioning

confidence: 99%

Methods of Micropatterning and Manipulation of Cells for Biomedical Applications

et al. 2017

View full text Add to dashboard Cite

Micropatterning and manipulation of mammalian and bacterial cells are important in biomedical studies to perform in vitro assays and to evaluate biochemical processes accurately, establishing the basis for implementing biomedical microelectromechanical systems (bioMEMS), point-of-care (POC) devices, or organs-on-chips (OOC), which impact on neurological, oncological, dermatologic, or tissue engineering issues as part of personalized medicine. Cell patterning represents a crucial step in fundamental and applied biological studies in vitro, hence today there are a myriad of materials and techniques that allow one to immobilize and manipulate cells, imitating the 3D in vivo milieu. This review focuses on current physical cell patterning, plus chemical and a combination of them both that utilizes different materials and cutting-edge micro-nanofabrication methodologies.

show abstract

Section: Techniques and Methodsmentioning

confidence: 99%

Methods of Micropatterning and Manipulation of Cells for Biomedical Applications

et al. 2017

View full text Add to dashboard Cite

show abstract

“…By means of self-assembling, the MPs can be patterned and positioned registry accurately for the usage in electronics, optics, sensors, biology, etc. [29][30][31][32][33][34][35][36] Generally, self-assembly is worked with the assistance of external forces, such as magnetic, electrostatic, capillary, or friction forces, which guide MPs orienting toward the directed position to form one-, two-, and threedimensional structures. [37][38][39][40][41][42][43][44][45] Among these, rubbing is a relatively common method for obtaining self-assembled MPs.…”

Section: Introductionmentioning

confidence: 99%

Capillary Self‐Assembly Register Microspheres to Fabricate Anisotropic Conductive Film Used for Ultra‐Fine Pitch Stable Electrical Interfacing Interconnection

Pan

Zhang

Cheng

et al. 2023

Adv Materials Technologies

View full text Add to dashboard Cite

The trend to integrate more electronic components in a limited space range motivates the development of advanced electronic packaging. Conventional electronic packaging is difficult to perform in stable interfacing interconnection pitch below 7 µm due to the possibility of short‐circuit problems. Herein, a kind of anisotropic conductive film (μ‐ACF) containing periodically arranged conductive microspheres for microscale pitch electrical interfacing interconnection is developed. The periodic arrangement can avert the contact of conductive microspheres, thus enabling stable interconnection with a pitch as small as 5 µm. By coating conductive microspheres with a layer of silicon insulation, the pitch can be further reduced to 3 µm, which can prevent the formation of conductive pathways between electrodes even if they come into contact with each other. Such high‐quality arrangement is achieved by lithography and capillary self‐assembly method, which is expected to be used in high‐throughput production. Thanks to the delicate design, the μ‐ACF can achieve a low contact resistance of 4.62 mΩ mm−2 and a spatial resolution of 3 µm without short‐circuit failure. The spatial resolution can be further improved by adjusting the size of conductive microspheres, which is conducive to the development of highly integrated devices.

show abstract

“…Other methods for cell patterning include soft lithographic techniques, such as microcontact printing [28,29], where cells adhere to selectively biochemically treated areas and form the printed pattern, and microwell trapping [30], where single-cell entrapment occurs in micropores or a defined diameter after the deposition of a cell suspension before the excess medium is removed. Although single cells are trapped, these methods do not have the ability to typically position multiple cell types in precise arrangements, and offer little flexibility in cell size variation, pattern shape, or spacing.…”

Section: Introductionmentioning

confidence: 99%

Patterning of Particles and Live Cells at Single Cell Resolution

et al. 2020

View full text Add to dashboard Cite

The ability to manipulate and selectively position cells into patterns or distinct microenvironments is an important component of many single cell experimental methods and biological engineering applications. Although a variety of particles and cell patterning methods have been demonstrated, most of them deal with the patterning of cell populations, and are either not suitable or difficult to implement for the patterning of single cells. Here, we describe a bottom-up strategy for the micropatterning of cells and cell-sized particles. We have configured a micromanipulator system, in which a pneumatic microinjector is coupled to a holding pipette capable of physically isolating single particles and cells from different types, and positioning them with high accuracy in a predefined position, with a resolution smaller than 10 µm. Complementary DNA sequences were used to stabilize and hold the patterns together. The system is accurate, flexible, and easy-to-use, and can be automated for larger-scale tasks. Importantly, it maintains the viability of live cells. We provide quantitative measurements of the process and offer a file format for such assemblies.

show abstract

High throughput micropatterning of interspersed cell arrays using capillary assembly

Cited by 11 publications

References 30 publications

Methods of Micropatterning and Manipulation of Cells for Biomedical Applications

Methods of Micropatterning and Manipulation of Cells for Biomedical Applications

Capillary Self‐Assembly Register Microspheres to Fabricate Anisotropic Conductive Film Used for Ultra‐Fine Pitch Stable Electrical Interfacing Interconnection

Patterning of Particles and Live Cells at Single Cell Resolution

Contact Info

Product

Resources

About