High-Throughput Microfluidic Device for LAMP Analysis of Airborne Bacteria

Jiang, Xiran; Jing, Wenwen; Sun, Xiaoting; Liu, Qi; Yang, Chunguang; Liu, Sixiu; Qin, Kairong; Sui, Guodong

doi:10.1021/acssensors.6b00282

Cited by 40 publications

(31 citation statements)

References 22 publications

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“…However, there is a lack of microfluidic data for atmospherically relevant INPs. Further to this, although bio-aerosol sampling has been demonstrated via the use of microfluidic devices for the detection of aerosol chemical composition (Noblitt et al 2009 ; Metcalf et al 2016 ) and airborne pathogens (Jing et al 2013 , 2014 ; Ma et al 2016 ; Bian et al 2016 ; Choi et al 2017 ; Jiang et al 2016a , b ), including on-site monitoring (e.g. in hospitals) (Noblitt et al 2009 ; Jiang et al 2016c ; Liu et al 2016 ), there is a notable lack of microfluidic applications for the study of the atmospheric sciences, in particular for the measurements of atmospheric INPs.…”

Section: Introductionmentioning

confidence: 99%

The study of atmospheric ice-nucleating particles via microfluidically generated droplets

Tarn

Sikora

Porter

et al. 2018

Microfluid Nanofluid

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Ice-nucleating particles (INPs) play a significant role in the climate and hydrological cycle by triggering ice formation in supercooled clouds, thereby causing precipitation and affecting cloud lifetimes and their radiative properties. However, despite their importance, INP often comprise only 1 in 103–106 ambient particles, making it difficult to ascertain and predict their type, source, and concentration. The typical techniques for quantifying INP concentrations tend to be highly labour-intensive, suffer from poor time resolution, or are limited in sensitivity to low concentrations. Here, we present the application of microfluidic devices to the study of atmospheric INPs via the simple and rapid production of monodisperse droplets and their subsequent freezing on a cold stage. This device offers the potential for the testing of INP concentrations in aqueous samples with high sensitivity and high counting statistics. Various INPs were tested for validation of the platform, including mineral dust and biological species, with results compared to literature values. We also describe a methodology for sampling atmospheric aerosol in a manner that minimises sampling biases and which is compatible with the microfluidic device. We present results for INP concentrations in air sampled during two field campaigns: (1) from a rural location in the UK and (2) during the UK’s annual Bonfire Night festival. These initial results will provide a route for deployment of the microfluidic platform for the study and quantification of INPs in upcoming field campaigns around the globe, while providing a benchmark for future lab-on-a-chip-based INP studies.Electronic supplementary materialThe online version of this article (10.1007/s10404-018-2069-x) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

The study of atmospheric ice-nucleating particles via microfluidically generated droplets

Tarn

Sikora

Porter

et al. 2018

Microfluid Nanofluid

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“…developed a MFs system that could capture and enrich airborne pathogens ( Staphylococcus aureus ) and detect them using LAMP. [ 108 ] The analysis time required from loading the bio‐aerosol to LAMP amplification was about 1.5 h with 24 cells per reaction as the detection limit for S. aureus . The specificity of the test was performed using five different air‐borne bacteria.…”

Section: Mfs For Environmental Monitoringmentioning

confidence: 99%

Emerging Trends in Microfluidics Based Devices

Solanki

Pandey

Gupta

et al. 2020

Biotechnology Journal

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One of the major challenges for scientists and engineers today is to develop technologies for the improvement of human health in both developed and developing countries. However, the need for cost-effective, high-performance diagnostic techniques is very crucial for providing accessible, affordable, and high-quality healthcare devices. In this context, microfluidic-based devices (MFDs) offer powerful platforms for automation and integration of complex tasks onto a single chip. The distinct advantage of MFDs lies in precise control of the sample quantities and flow rate of samples and reagents that enable quantification and detection of analytes with high resolution and sensitivity. With these excellent properties, microfluidics (MFs) have been used for various applications in healthcare, along with other biological and medical areas. This review focuses on the emerging demands of MFs in different fields such as biomedical diagnostics, environmental analysis, food and agriculture research, etc., in the last three or so years. It also aims to reveal new opportunities in these areas and future prospects of commercial MFDs.

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“…Additionally, these peptides were attached to underlying electrodes, and bacterial detection was measured by resulting changes in resistance with a limit of detection of 10 CFU/mL [ 70 ]. Other devices have been designed to identify pathogenic bacteria and bacteria toxins within air samples [ 71 , 72 ]. Bian et al trapped the bacteria Vibrio parahemolyticus within a microfluidic trapping device and performed mass spectrometry to identify the bioaerosols excreted by the bacteria [ 71 ].…”

Section: Disease Detectionmentioning

confidence: 99%

“…Bian et al trapped the bacteria Vibrio parahemolyticus within a microfluidic trapping device and performed mass spectrometry to identify the bioaerosols excreted by the bacteria [ 71 ]. Jiang et al developed a device to test air samples by flowing air spiked with bacteria through a microfluidic device coated with LAMP reagents to detect Staphylococcus aureus as well as four other common airborne bacteria with a limit of detection of 24 CFU per microfluidic channel for air spiked with S. aureus [ 72 ].…”

Section: Disease Detectionmentioning

confidence: 99%

Microfluidic and Paper-Based Devices for Disease Detection and Diagnostic Research

Campbell

Balhoff

Landwehr

et al. 2018

IJMS

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Recent developments in microfluidic devices, nanoparticle chemistry, fluorescent microscopy, and biochemical techniques such as genetic identification and antibody capture have provided easier and more sensitive platforms for detecting and diagnosing diseases as well as providing new fundamental insight into disease progression. These advancements have led to the development of new technology and assays capable of easy and early detection of pathogenicity as well as the enhancement of the drug discovery and development pipeline. While some studies have focused on treatment, many of these technologies have found initial success in laboratories as a precursor for clinical applications. This review highlights the current and future progress of microfluidic techniques geared toward the timely and inexpensive diagnosis of disease including technologies aimed at high-throughput single cell analysis for drug development. It also summarizes novel microfluidic approaches to characterize fundamental cellular behavior and heterogeneity.

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High-Throughput Microfluidic Device for LAMP Analysis of Airborne Bacteria

Cited by 40 publications

References 22 publications

The study of atmospheric ice-nucleating particles via microfluidically generated droplets

The study of atmospheric ice-nucleating particles via microfluidically generated droplets

Emerging Trends in Microfluidics Based Devices

Microfluidic and Paper-Based Devices for Disease Detection and Diagnostic Research

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