High-throughput microfluidic compressibility cytometry using multi-tilted-angle surface acoustic wave

Wu, Yanqi; Stewart, Alastair G.; Lee, Peter Vee Sin

doi:10.1039/d1lc00186h

Cited by 18 publications

(14 citation statements)

References 66 publications

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“…MTs are well known for their stabilising function of cell membrane protrusions during cell migration 52 and can also modulate single-cell major-axis length 53 . Indeed, MTs embedded in the actomyosin network can bear significant compressive forces 54 , 55 and modulate cell compressibility 56 , or bear the compression driven by the constriction of cardiomyocytes 57 . Thus, apical MTs could directly resist the pre-existing cortical tension in the midline cells and their disassembly would be sufficient to trigger cell constriction.…”

Section: Discussionmentioning

confidence: 99%

Microtubule disassembly by caspases is an important rate-limiting step of cell extrusion

2022

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The expulsion of dying epithelial cells requires well-orchestrated remodelling steps to maintain tissue sealing. This process, named cell extrusion, has been mostly analysed through the study of actomyosin regulation. Yet, the mechanistic relationship between caspase activation and cell extrusion is still poorly understood. Using the Drosophila pupal notum, a single layer epithelium where extrusions are caspase-dependent, we showed that the initiation of cell extrusion and apical constriction are surprisingly not associated with the modulation of actomyosin concentration and dynamics. Instead, cell apical constriction is initiated by the disassembly of a medio-apical mesh of microtubules which is driven by effector caspases. Importantly, the depletion of microtubules is sufficient to bypass the requirement of caspases for cell extrusion, while microtubule stabilisation strongly impairs cell extrusion. This study shows that microtubules disassembly by caspases is a key rate-limiting step of extrusion, and outlines a more general function of microtubules in epithelial cell shape stabilisation.

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Section: Discussionmentioning

confidence: 99%

Microtubule disassembly by caspases is an important rate-limiting step of cell extrusion

2022

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“…Generally, the acoustic radiation force and the Stokes’ drag force on micrometer-scale particles/cells as expressed in eqs and in acoustofluidics. It is theoretically proved that the F r value on the particles mainly depends on the particle, fluid and acoustic parameters, among which the particle size has the greatest influence on the acoustic radiation and has a cubic relationship . However, the density and compressibility of the particle and the fluid medium make up the acoustic contrast factor φ acting on F r and play a nondominant role: F r = − true( π p 0 2 V 0 β f 2 λ true) φ sin false( 2 k L false) φ false( β , ρ false) = 5 ρ p − 2 ρ f 2 ρ p + ρ f − β p β f where p 0 is the acoustic pressure, λ the wavelength, V 0 the volume of the particle, k the wavenumber, and L the distance from the particle position to the pressure node (antinode).…”

Section: Working Principlementioning

confidence: 99%

“…It is theoretically proved that the F r value on the particles mainly depends on the particle, fluid and acoustic parameters, among which the particle size has the greatest influence on the acoustic radiation and has a cubic relationship. 18 However, the density and compressibility of the particle and the fluid medium make up the acoustic contrast factor φ acting on F r and play a nondominant role:…”

Section: ■ Working Principlementioning

confidence: 99%

“…The calibration between cell compressibility, size, and acoustic radiation force is realized to improve the detection sensitivity. Different from the static observation of bioparticle migration trajectories, Wu et al 18 developed a high-throughput compressive cytometry approach using multitilted-angle surface acoustic waves, which recorded the position of collision with the sidewall to estimate the compressibility of a cell more intuitively.…”

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confidence: 99%

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Manipulation of Particle/Cell Based on Compressibility in a Divergent Microchannel by Surface Acoustic Wave

Xue

et al. 2023

Anal. Chem.

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The mechanical properties (compressibility or deformability) of cells are closely related to their death, migration, and differentiation. Accurate separation and manipulation of bioparticles based on these mechanical properties are still a challenging in the field of acoustofluidics. In this work, based on surface acoustic waves (SAW) and divergent microchannels, we developed a new method for separating and detecting particles or cells with different compressibility. The difference in acoustic radiation force (F r) caused by compressibility are gradually amplified and accumulated by decreasing the flow velocity, and they are finally reflected in the particle migration distance. During the transverse migration process, the alternating dominance of the acoustic radiation force and the Stokes resistance force (F s) drives the particles to create three typical migration patterns: intermittent migration, compound migration, and near-wall migration. In the present tilted SAW device, a 91% separation success rate of ∼10 μm polystyrene (PS) and polydimethylsiloxane (PDMS) particles can be achieved by optimizing the acoustic field input power and the fluid velocity. The application potential of the present divergent microchannel is validated by separating the myelogenous leukemia cell K562 and the natural killer cell NK92 that have similar densities and sizes (∼15 μm) but different compressibility. The results of this work are expected to provide valuable insights into the acoustofluidics separation and detection of the cells that are with different compressibility.

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“…Promising systems for the manipulation of particles based on e.g. focused SAW beams 12,50,51 or tilted arrangements 22,40,[52][53][54] of the SAWs introduce additional degrees of freedom, which can significantly affect the fluid flow and require a separate consideration. Based on the schematic representation in Fig.…”

Section: Introductionmentioning

confidence: 99%

On the acoustically induced fluid flow in particle separation systems employing standing surface acoustic waves – Part II

2022

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High-throughput microfluidic compressibility cytometry using multi-tilted-angle surface acoustic wave

Abstract: Cellular mechanical properties (e.g. compressibility) are important biophysical markers in relation to cellular processes and functionality. Among the methods for cell mechanical measurement, acoustofluidic methods appear to be advantageous due...

Cited by 18 publications

References 66 publications

Microtubule disassembly by caspases is an important rate-limiting step of cell extrusion

Microtubule disassembly by caspases is an important rate-limiting step of cell extrusion

Manipulation of Particle/Cell Based on Compressibility in a Divergent Microchannel by Surface Acoustic Wave

On the acoustically induced fluid flow in particle separation systems employing standing surface acoustic waves – Part II

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