High throughput metabolomics explores the mechanism of Jigucao capsules in treating Yanghuang syndrome rats using ultra-performance liquid chromatography quadrupole time of flight coupled with mass spectrometry

Li, Taiping; He, Yanjin; Zhang, Mengli; Tan, Zhien; Zhang, Aihua; Ou, Min; Huang, Danna; Wu, Fangfang; Wang, Xijun

doi:10.1016/j.jchromb.2022.123185

Cited by 6 publications

(7 citation statements)

References 20 publications

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“…The source of the serum samples was based on the samples of our previous metabolomics study on JGCC in the treatment of DHJS [ 6 , 7 ]. Eight-week-old clean-grade adult male SD rats were purchased from Guangxi Medical University (license number SYXK (Gui) 2020-0014).…”

Section: Methodsmentioning

confidence: 99%

“…It mainly presents with three yellow symptoms, including yellow eyes, yellow body and yellow urine, as well as pathological manifestations such as thirst and fever, inappetence, disgusting vomits and poor defecation [ 5 ]. Metabolomics studies have shown that JGCC has a significant therapeutic effect on rats with DHJS by acting on some abnormal metabolic pathways such as pentose and glucuronate interconversions, arachidonic acid metabolism and primary bile acid biosynthesis [ 6 , 7 ]. On the other hand, modern pharmacological studies have shown that JGCC combined with entecavir antiviral treatment of patients with chronic hepatitis B can enhance its antiviral effects and delay the progression of liver fibrosis by reducing serum transforming growth factor-β1, transaminase, total serum bilirubin and other liver function indicators [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

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Quality Markers’ Discovery and Quality Evaluation of Jigucao Capsule Using UPLC-MS/MS Method

Tan

et al. 2023

Molecules

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Jigucao capsules (JGCC) have the effects of soothing the liver and gallbladder and clearing heat and detoxification. It is a good medicine for treating acute and chronic hepatitis cholecystitis with damp heat of the liver and gallbladder. However, the existing quality standard of JGCC does not have content determination items, which is not conducive to quality control. In this study, serum pharmacochemistry technology and UNIFI data processing software were used to identify the blood prototype components and metabolites under the condition of the obvious drug effects of JGCC, and the referenced literature reports and the results from in vitro analysis of JGCC in the early stage revealed a total of 43 prototype blood components and 33 metabolites in JGCC. Quality markers (Q-markers) were discovered, such as abrine, trigonelline, hypaphorine and isoschaftoside. In addition, ultra-high-performance liquid chromatography–triple quadrupole mass spectrometry (UPLC-QQQ-MS) was used to determine the active ingredients in JGCC. The components of quantitative analysis have good correlation in the linear range with R2 ≥ 0.9993. The recovery rate is 93.15%~108.92% and the relative standard deviation (RSD) is less than 9.48%. The established UPLC-MS/MS quantitative analysis method has high sensitivity and accuracy, and can be used for the quality evaluation of JGCC.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Quality Markers’ Discovery and Quality Evaluation of Jigucao Capsule Using UPLC-MS/MS Method

Tan

et al. 2023

Molecules

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“…The function of JGCTs, JGCCs and FFJGCC are to soothe the liver, promote bile secretion, clear heat, and detoxify. 3 Due to the complex composition of these drugs, it is essential to establish rapid and reliable analytical methods for quantitative and qualitative analysis purpose.…”

Section: Introductionmentioning

confidence: 99%

“…In the reported references, many studies have focused on the application of analytical techniques to the quantification of target compounds in Abri Herba and/or Abri mollis Herba, 1,[12][13][14] and explored the mechanism of JGCC. 3,15 In the best of our knowledge, there was only one report focused on the characterization of multiple target compounds in JGCC. 16 Therefore, it is required to develop a suitable method for the quantitative analysis of multiple components in these medicines.…”

Section: Introductionmentioning

confidence: 99%

“…The main ingredients of the above Jigucao capsules (JGCCs) and FufangJigucao capsules (FFJJCCs) are Abri mollis Herba, Panax Notoginseng, Bovis Calculus Artifactus, Susscrofa domestica Brisson, Origanum vulgare L, Paeoniae Radix Alba, Jujubae Fructus, Gardeniae Fructus, Artemisiae Scopariae Herba, Lycii Fructus. The function of JGCTs, JGCCs and FFJGCC are to soothe the liver, promote bile secretion, clear heat, and detoxify 3 . Due to the complex composition of these drugs, it is essential to establish rapid and reliable analytical methods for quantitative and qualitative analysis purpose.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous quantitative and qualitative analysis of three kinds of Jigucao related pharmaceuticals by ultra‐high performance liquid chromatography combined with chemometrics method

Yang,

Li,

Qiu

et al. 2023

Flavour & Fragrance J

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Abri Herba (Jigucao) and Abri mollis Herba (Maojigucao) are derived from dried plants of Abrus cantoniensis Hance (Leguminosae) and Abrus mollis without pods. Abri Herba is commonly used in Jigucao tablets; Abri mollis Herba is usually used in Jigucao capsules and FufangJigucao capsules. Surprising, there are few reports focused on the quantitative control of these mentioned Jigucao related pharmaceuticals. This research endeavour has harnessed the formidable power of cutting‐edge ACQUITY UPLC technology to formulate a groundbreaking methodology for the simultaneous quantification of 10 target compounds in Jigucao capsules, Jigucao tablets and FufangJigucao capsules. The primary aim is to bolster quality control and precise identification within the realm of these pharmaceutical products. Separation was performed on an ACQUITY UPLC HSS T3 Column (2.1 × 100 mm, 1.8 μm), gradient elution was performed with 0.1% formic acid in water and acetonitrile. A column temperature of 40°C acts as our guiding compass, and DAD detection scans the spectral landscape in the wavelength range of 210–400 nm. The concentrations of the 10 target components can be used to identify Jigucao tablets, Jigucao capsules and FufangJigucao capsules through the art of chemometrics. The results showed that the established method for determination of 10 target compounds was accurate and reliable with good linearity (r ≥ .9948) and satisfactory recoveries (82.91%–108.6%). The identification of the three Jigucao related pharmaceuticals can be performed using chemometrics based on the 10 target compounds. Thus, our study not only showcases a pioneering analytical approach but also unveils a new dimension in the identification of these pharmaceutical treasures.

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Zingiber officinale and Panax ginseng ameliorate ulcerative colitis in mice via modulating gut microbiota and its metabolites

Wan

Yang

et al. 2022

Journal of Chromatography B

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High throughput metabolomics explores the mechanism of Jigucao capsules in treating Yanghuang syndrome rats using ultra-performance liquid chromatography quadrupole time of flight coupled with mass spectrometry

Cited by 6 publications

References 20 publications

Quality Markers’ Discovery and Quality Evaluation of Jigucao Capsule Using UPLC-MS/MS Method

Quality Markers’ Discovery and Quality Evaluation of Jigucao Capsule Using UPLC-MS/MS Method

Simultaneous quantitative and qualitative analysis of three kinds of Jigucao related pharmaceuticals by ultra‐high performance liquid chromatography combined with chemometrics method

Zingiber officinale and Panax ginseng ameliorate ulcerative colitis in mice via modulating gut microbiota and its metabolites

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