High throughput measure of diversity in cytoplasmic and nuclear traits for unravelling geographic distribution of rosemary

Abstract

In the present work, variability of both cytoplasmic and nuclear microsatellite traits was investigated with the aim of characterizing a set of rosemary germplasm resources ( Salvia rosmarinus ). Most of the materials were collected in Italy and France. High‐resolution melting curves were compared each other computing their Euclidean distances and estimating the differences within their principal component as a measure of genetic diversity. Mantel correlation results combined to linear discriminant analysis allowed examined populations to be divided in four principal groups corresponding to four geographic areas, with few interesting and discussed exceptions. As rosemary propagates by seeds coming from insect mediated pollination, steady wild populations can be expected to be in panmictic equilibrium. Gained results confirmed and extended precedent characterization of rosemary genotypes and are compatible with the distribution of other Mediterranean species, as well as with the presence of a glacial refugium in the north‐east area of Sardinia previously described. As the officinal use of this aromatic shrub is spreading, characterization and conservation of wild Mediterranean germplasm is gaining strategic importance. A core collection of 100 genotypes was pointed out as suitable for a cheaper biodiversity ex situ preservation as well as for subsequent metabolic and linkage disequilibrium analyses.

Effects of Irrigation, Peat-Alternative Substrate and Plant Habitus on the Morphological and Production Characteristics of Sicilian Rosemary (Rosmarinus officinalis L.) Biotypes Grown in Pot

“…Rosemary presents a variety of different habitus types, morphological traits, flower colours and aromatic properties [80]. Our study confirms results reported by Flamini et al [81], who, in a recent study on the evaluation of the agronomic and production characteristics of two rosemary biotypes, state that the best performances are produced by biotypes with an erect habitus.…”

Application of High Resolution Melt analysis (HRM) for screening haplotype variation in a non-model plant genus: Cyclopia (Honeybush)

“…These studies, and most others applying HRM to non-model organisms (Dang et al, 2012;Li et al, 2012;Radvansky et al, 2011), set out to develop HRM primers having prior knowledge of the nucleotide variation under analyses. Unfortunately, such knowledge is generally not available for the study of non-model organisms and the application of HRM for detecting and genotyping of novel genetic variation in wild populations is still rare (Nunziata et al, 2019;Sillo et al, 2017). High Resolution Melt analysis appears to be an underutilized resource by phylogeographers.…”

Antifungal Activity of Salvia jordanii Against the Oral Thrush Caused by the Cosmopolitan Yeast Candida albicans among Elderly Diabetic Type 2 patients

“…The Mediterranean herb S. jordanii is one of the potential medicinal herbs especially against the fungal infections due to its rich source of anti-fungal components present [7][8][9][10]. S. jordanii was previously described as rosemary but the recent studies based upon its DNA sequencing classified into Salvia family [11]. The leaves of S. jordanii were collected and dried to extract the essential oil required for this work to check its antifungal activities against the collected specimens [12][13][14].…”