High throughput kinetic Vibrio fischeri bioluminescence inhibition assay for study of toxic effects of nanoparticles

Mortimer, Monika; Kasemets, Kaja; Heinlaan, Margit; Kurvet, Imbi; Kahru, Anne

doi:10.1016/j.tiv.2008.02.011

Cited by 146 publications

(84 citation statements)

References 17 publications

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“…The existing studies using V. fischeri have shown the toxic effect of various NPs (including ZnO) Strigul et al 2009). It should however be stressed that these studies have been conducted only for aqueous solutions Mortimer et al 2008). Our preliminary analysis of the toxicity also revealed a significant reduction in bioluminescence in the aqueous solution; at a concentration of 1 mg L −1 , the reduction in bioluminescence was 65 % for nano-ZnO50, 45 % for nano-ZnO100, and 60 % for bulkZnO.…”

Section: Discussionmentioning

confidence: 63%

The bioavailability and toxicity of ZnO and Ni nanoparticles and their bulk counterparts in different sediments

2016

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Purpose The intensive development of nanotechnology raises a question of the potential consequences of the presence of nanoparticles (NPs) in the different components of the environment, including sediments. The aim of this study was to evaluate the toxicity of nanoparticles of ZnO and Ni and their bulk counterparts in bottom sediments (SD1, SD2) with different properties collected from the Vistula River in Poland. Materials and methods Sediment samples with NPs at a concentration of 100 mg kg −1 were incubated for 17 months in the dark or under a photoperiod of 12 h light/12 h dark. The Microtox ® (bacteria, Vibrio fischeri) and OSTRACODTOXKIT F ™ (ostracods, Heterocypris incongruens) tests were used to evaluate toxicity. In addition, the contents of Zn and Ni were determined in extracts (H 2 O and CaCl 2 ) of the bottom sediments. Results and discussion The Zn concentration was much lower in the SD1 sediment with the addition of NPs/bulk particles (30-230 μg kg −1 ) compared to the SD2 sediment (280-1140 μg kg −1 ). The toxicity of ZnO and Ni was determined by the type of bottom sediment and the parameter studied.Both nano-and bulk-ZnO and Ni caused the mortality of H. incongruens at a level of 13.3-53.3 %. The influence of ZnO and Ni on the growth of H. incongruens was observed to be the opposite. ZnO resulted in growth stimulation, while Ni resulted in growth inhibition of H. incongruens. Both ZnO and Ni stimulated V. fisheri luminescence. In most cases, the incubation of ZnO and Ni under the photoperiod increased the toxicity or decreased the stimulation of V. fisheri bioluminescence and H. ingongruens growth compared to the darkincubated sediments. Conclusions The study provides new and important information on the ecotoxicological effects of ZnO and Ni nanoparticles in different sediments and under various environmental conditions that may be useful for the risk assessment of this new group of contaminants.

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Section: Discussionmentioning

confidence: 63%

The bioavailability and toxicity of ZnO and Ni nanoparticles and their bulk counterparts in different sediments

2016

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“…Earlier a series of physiological effects induced by ZnO-NPs have been observed in rainbow trout, Oncorhynchus mykiss [14], micro algae Pseudokir-chneriella subcapitata [15], crustacean Daphnia magna and Thermo-cephalus platyurus and bacteria Vibrio fischeri [16]. However, research on the toxicity of NPs are far from being complete, and the studies of potential adverse effects and related mechanisms exerted by NPs in the soil ecosystem are still limited [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

“…However, research on the toxicity of NPs are far from being complete, and the studies of potential adverse effects and related mechanisms exerted by NPs in the soil ecosystem are still limited [16][17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Bioaccumulation of ZnO-NPs in Earthworm Eisenia fetida (Savigny)

Gupta¹,

Yadav²

2014

J Bioremed Biodeg

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“…12 Although PEG-modified PEI has lower toxicity, its biological toxicity in vivo remains a difficult problem to tackle. [13][14][15][16] For this reason, we chose a new material polyamidoamine (PAMAM) to replace PEI. PAMAM is a novel class of nanoscopic and spherical polymer that has been proven to be an efficient gene carrier.…”

Section: Introductionmentioning

confidence: 99%

Second-generation aptamer-conjugated PSMA-targeted delivery system for prostate cancer therapy

Ding²,

Gao³

et al. 2011

IJN

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Background: miR-15a and miR-16-1 have been identified as tumor suppressor genes in prostate cancer, but their safe and effective delivery to target cells is key to the successful use of this therapeutic strategy. RNA aptamer A10 has been used as a ligand, targeting prostate cancer cells that express prostate-specific membrane antigen (PSMA). Compared with A10, the binding of the second-generation RNA aptamer, A10-3.2, to PSMA is more efficient. Methods: A10-3.2 was investigated as a PSMA-targeting ligand in the design of a polyamidoamine (PAMAM)-based microRNA (miR-15a and miR-16-1) vector to prostate cancer cells. Using polyethyleneglycol (PEG) as a spacer, PAMAM was conjugated to aptamer (PAMAM-PEG-APT) and used as a vehicle for miRNA target delivery. Results: Luciferase assays of pGL-3 expression against PC3 (PSMA − ) and LNCaP (PSMA + ) cells demonstrated that the transfection efficiency of the synthesized DNA/PAMAM-PEG-APT complex was higher than that of the DNA/PAMAM-PEG complex. In addition, cell viability assays of LNCaP (PSMA + ) cells showed that, with a N/P ratio of 15:1, the IC 50 value of miRNA/PAMAM-PEG-APT was approximately 4.7-fold lower than that of miRNA/PAMAM-PEG. Conclusion: This PSMA-targeted system may prove useful in widening the therapeutic window and allow for selective killing of prostate cancer cells.

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High throughput kinetic Vibrio fischeri bioluminescence inhibition assay for study of toxic effects of nanoparticles

Cited by 146 publications

References 17 publications

The bioavailability and toxicity of ZnO and Ni nanoparticles and their bulk counterparts in different sediments

The bioavailability and toxicity of ZnO and Ni nanoparticles and their bulk counterparts in different sediments

Bioaccumulation of ZnO-NPs in Earthworm Eisenia fetida (Savigny)

Second-generation aptamer-conjugated PSMA-targeted delivery system for prostate cancer therapy

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