High‐throughput droplet microfluidics screening platform for selecting fast‐growing and high lipid‐producing microalgae from a mutant library

Kim, Hyun Soo; Hsu, Shih-Chi; Han, Song‐I; Thapa, Hem R.; Guzman, Adrian R.; Browne, Daniel R.; Tatli, Mehmet; Devarenne, Timothy P.; Stern, David B.; Han, Arum

doi:10.1002/pld3.11

Cited by 66 publications

(54 citation statements)

References 54 publications

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“…However, conventional approaches utilized for strain selection mostly rely on complicated procedures that are labor intensive and time consuming [21,24]. In this regard, Kim et al [25] studied the novel advances in the development of microfluidic systems for microalgae biotechnology. Similarly, Challagulla et al [26] reviewed the application of the nuclear magnetic resonance spectroscopy, fluorescent lipid-soluble dyes, Raman spectroscopy, near-infrared spectroscopy, and Fourier transform techniques for analyzing lipid contents in microalgae.…”

Section: Introductionmentioning

confidence: 99%

Improving ‘Lipid Productivity’ in Microalgae by Bilateral Enhancement of Biomass and Lipid Contents: A Review

Shokravi

Ong

et al. 2020

Sustainability

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Microalgae have received widespread interest owing to their potential in biofuel production. However, economical microalgal biomass production is conditioned by enhancing the lipid accumulation without decreasing growth rate or by increasing both simultaneously. While extensive investigation has been performed on promoting the economic feasibility of microalgal-based biofuel production that aims to increase the productivity of microalgae species, only a handful of them deal with increasing lipid productivity (based on lipid contents and growth rate) in the feedstock production process. The purpose of this review is to provide an overview of the recent advances and novel approaches in promoting lipid productivity (depends on biomass and lipid contents) in feedstock production from strain selection to after-harvesting stages. The current study comprises two parts. In the first part, bilateral improving biomass/lipid production will be investigated in upstream measures, including strain selection, genetic engineering, and cultivation stages. In the second part, the enhancement of lipid productivity will be discussed in the downstream measure included in the harvesting and after-harvesting stages. An integrated approach involving the strategies for increasing lipid productivity in up- and down-stream measures can be a breakthrough approach that would promote the commercialization of market-driven microalgae-derived biofuel production.

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Section: Introductionmentioning

confidence: 99%

Improving ‘Lipid Productivity’ in Microalgae by Bilateral Enhancement of Biomass and Lipid Contents: A Review

Shokravi

Ong

et al. 2020

Sustainability

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“…In addition, the droplet-based microfluidics can provide encapsulation of a single cell within a droplet, which allows for high-throughput single cell screening and analysis capabilities [5,6]. The droplet-based microfluidic systems have been successfully utilized in a variety of applications, such as single cell analysis/screening [7][8][9], protein crystallization [10], polymer chain reaction (PCR) [11], drug discovery [12][13][14], and microorganism reactions [15,16] to name a few. For all of these applications, producing droplets with controlled sizes as well as at controlled generation rates are key factors to obtain consistent and robust results.…”

Section: Introductionmentioning

confidence: 99%

The Effect of Oil Viscosity on Droplet Generation Rate and Droplet Size in a T-Junction Microfluidic Droplet Generator

et al. 2019

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There have been growing interests in droplet-based microfluidics due to its capability to outperform conventional biological assays by providing various advantages, such as precise handling of liquid/cell samples, fast reaction time, and extremely high-throughput analysis/screening. The droplet-based microfluidics utilizes the interaction between the interfacial tension and the fluidic shear force to break continuous fluids into uniform-sized segments within a microchannel. In this paper, the effect of different viscosities of carrier oil on water-in-oil emulsion, particularly how droplet size and droplet generation rate are affected, has been investigated using a commonly used T-junction microfluidic droplet generator design connected to a pressure-controlled pump. We have tested mineral oils with four different viscosities (5, 7, 10, and 15 cSt) to compare the droplet generation under five different flow pressure conditions (i.e., water flow pressure of 30–150 mbar and oil flow pressure of 40–200 mbar). The results showed that regardless of the flow pressure levels, the droplet size decreased as the oil viscosity increased. Average size of the droplets decreased by approximately 32% when the viscosity of the oil changed from 5 to 15 cSt at the flow pressure of 30 mbar for water and 40 mbar for oil. Interestingly, a similar trend was observed in the droplet generation rate. Droplet generation rate and the oil viscosity showed high linear correlation (R2 = 0.9979) at the water flow pressure 30 mbar and oil flow pressure 40 mbar.

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“…In DEP-based cell manipulation and sorting, the movement of polarizable particles, such as cells, in an inhomogeneous electric field is dependent on their intrinsic dielectric properties in comparison to Despite these advances, many challenges still remain [15][16][17]. Identifying and developing strains with higher productivity, improved understanding of their behaviors under diverse cultivation conditions, and better insights into the heterogeneous population are all some of the key advances that have to be made in the upstream process of microalgae biotechnology and bioprocessing [16,[18][19][20][21][22]]. Yet, these development processes are often time-consuming and labor-intensive, posing as a significant bottleneck.…”

Section: Dep Technology For Cell Population Analysismentioning

confidence: 99%

“…Many such applications have been on selecting and obtaining high-lipid-producing strains, as microalgae-based lipid production is an important area towards renewable bioenergy applications [19,65]. However, there are other examples of high-throughput screening than just lipid production.…”

Section: Strain Selection Through Screeningmentioning

confidence: 99%

Separation, Characterization, and Handling of Microalgae by Dielectrophoresis

et al. 2020

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Microalgae biotechnology has a high potential for sustainable bioproduction of diverse high-value biomolecules. Some of the main bottlenecks in cell-based bioproduction, and more specifically in microalgae-based bioproduction, are due to insufficient methods for rapid and efficient cell characterization, which contributes to having only a few industrially established microalgal species in commercial use. Dielectrophoresis-based microfluidic devices have been long established as promising tools for label-free handling, characterization, and separation of broad ranges of cells. The technique is based on differences in dielectric properties and sizes, which results in different degrees of cell movement under an applied inhomogeneous electrical field. The method has also earned interest for separating microalgae based on their intrinsic properties, since their dielectric properties may significantly change during bioproduction, in particular for lipid-producing species. Here, we provide a comprehensive review of dielectrophoresis-based microfluidic devices that are used for handling, characterization, and separation of microalgae. Additionally, we provide a perspective on related areas of research in cell-based bioproduction that can benefit from dielectrophoresis-based microdevices. This work provides key information that will be useful for microalgae researchers to decide whether dielectrophoresis and which method is most suitable for their particular application.

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High‐throughput droplet microfluidics screening platform for selecting fast‐growing and high lipid‐producing microalgae from a mutant library

Cited by 66 publications

References 54 publications

Improving ‘Lipid Productivity’ in Microalgae by Bilateral Enhancement of Biomass and Lipid Contents: A Review

Improving ‘Lipid Productivity’ in Microalgae by Bilateral Enhancement of Biomass and Lipid Contents: A Review

The Effect of Oil Viscosity on Droplet Generation Rate and Droplet Size in a T-Junction Microfluidic Droplet Generator

Separation, Characterization, and Handling of Microalgae by Dielectrophoresis

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