High-throughput DNA sequencing of microbiota at interproximal sites

Abstract

Objective: The oral microbiota has been deeply studied by high-throughput sequencing techniques. However, although the interproximal regions have one of the highest caries rates in the oral cavity, information about the bacterial composition at those sites is scarce. Methods: In this study, we used 16S rRNA Illumina sequencing to describe the microbiota associated to interproximal regions at two time points. In addition, dental plaque samples at the vestibular and lingual surfaces from the same teeth were also analysed at the two time points. Results: Interproximal-associated microbiota was found to be similar to already described bacterial communities in other mouth niches. Streptoccocus, Veillonella, Rothia, Actinomyces, Neisseria, Haemophilus and Fusobacterium were the most abundant genera in this oral region. Statistical analyses showed that the microbiota from interproximal sites was more similar to that sampled from the vestibular surfaces than to the lingual surfaces. Interestingly, many potentially cariogenic bacteria such as Scardovia, Atopobium or Selenomonas were overrepresented in the interproximal regions in comparison with vestibular and lingual sites. Conclusion: The microbiota at interproximal regions appears to be specific and stable through time. Potentially pathogenic bacteria may increase caries development risk and gingival inflammation at those sites.

Topic Application of the Probiotic Streptococcus dentisani Improves Clinical and Microbiological Parameters Associated With Oral Health

“…Reads were analyzed as previously described (Carda-Diéguez et al, 2019 ) using the Dada2 pipeline (Callahan et al, 2016 ). Briefly, we filtered, end-trimmed, denoise, and merge paired reads before adapters and primers were filtered out.…”

Study on the community structure and function of symbiotic bacteria from different growth and developmental stages of Hypsizygus marmoreus

“…16S rDNA PCR amplification and high-throughput sequencing (HiSeq) [36,37] Total DNA was extracted from H. marmoreus hyphae with a Genomic DNA Kit (TransGen Biotech Co., Ltd., Beijing, China). When sufficient quantities (400-500 ng) of highquality community DNA samples were available, the V3-V4 region of the 16S rDNA was amplified with universal primers (338 F: 5′-ACTCCTACGGGAGGCAGCA-3′; 806 R: 5′-GGACTACHVGGGTWTCTAAT-3′) in 50 uL reaction volume.…”

Study on the community structure and function of symbiotic bacteria at different growth and developmental stages of Hypsizygus marmoreus

“…16S rDNA PCR ampli cation and high-throughput sequencing (HiSeq) [34,35] Total DNA was extracted from H. marmoreus hyphae with a Genomic DNA Kit (TransGen Biotech Co., Ltd., Beijing, China). When su cient quantities (400-500 ng) of high-quality community DNA samples were available, the V3-V4 region of the 16S rDNA was ampli ed with universal primers (338 F: 5'-ACTCCTACGGGAGGCAGCA-3'; 806 R: 5'-GGACTACHVGGGTWTCTAAT-3') in 50 uL reaction volume.…”