High-throughput discovery of highly selective reversible hMAO-B inhibitors based on at-line nanofractionation

Fan, Yu; Wang, Jincai; Jian, Jingyi; Wen, Yalei; Li, Jiahao; Tian, Hao; Crommen, Jacques; Bi, Wei; Zhang, Tingting; Jiang, Zhengjin

doi:10.1016/j.apsb.2024.01.020

Cited by 3 publications

(1 citation statement)

References 63 publications

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“…These components were identified using Compound Discoverer 3.3 software and its database suite, which includes ChemSpider, mzCloud, mzVault, and OTCML. It merits emphasis that these databases are extensively leveraged to elucidate the components used in TCM ( Qian et al, 2022 ; Fan et al, 2024 ). To investigate the associated pharmacological effects, wind-cold modeling equipment (TPC 460, ALISN Shanghai Co., Ltd.) was used to establish a standardized mouse model for investigating the anti-inflammatory, antioxidant, and antiapoptotic effects of CCD on wind-cold.…”

Section: Discussionmentioning

confidence: 99%

Decoction derived from Allium ascalonicum L. bulbs and Sojae Semen Praeparatum alleviates wind-cold-type common cold via Nrf2/HO-1 pathway and modulation of Lactobacillus murinus level

Jiang,

Wei,

Zhou

et al. 2024

Front. Pharmacol.

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BackgroundCong-Chi decoction (CCD) is made using Allium ascalonicum L. (shallot) bulbs and Sojae Semen Praeparatum (SSP). Shallot bulbs and SSP are both used regularly in traditional Chinese medicine; however, there are no recent pharmacological studies on their synergistic effects. Despite their roles in the treatment of the common cold for thousands of years, their pharmacological mechanisms of action against wind-cold-type common cold are yet to be explored comprehensively.MethodsA mouse model was standardized using wind-cold modeling equipment to study the anti-inflammatory, antioxidant, and antiapoptotic effects of CCD. Then, 16S rRNA sequencing was employed to analyze the association between Lactobacillus murinus and changes in body temperature. Additionally, the antipyretic effects of L. murinus were validated via animal experiments.ResultsThe results indicate that CCD improves the symptoms of wind-cold by reducing fever, levels of pro-inflammatory factors, and cellular apoptosis, as well as increasing the blood leukocyte and lymphocyte counts, thereby alleviating lung tissue damage. The effects of CCD are mediated by upregulation of pulmonary Nrf2 and HO-1 expressions, thereby reducing oxidative damage in the lungs, in addition to other anti-inflammatory mechanisms. Furthermore, CCD increases the abundance of L. murinus in the intestinal tract. The animal experiments confirm that L. murinus ameliorates fever in mice.ConclusionCCD exhibits remarkable antioxidant and anti-inflammatory properties for effectively treating wind-cold-type common cold. Furthermore, its regulatory effects on L. murinus represent a novel mechanism for product development.

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Section: Discussionmentioning

confidence: 99%

Decoction derived from Allium ascalonicum L. bulbs and Sojae Semen Praeparatum alleviates wind-cold-type common cold via Nrf2/HO-1 pathway and modulation of Lactobacillus murinus level

Jiang,

Wei,

Zhou

et al. 2024

Front. Pharmacol.

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Establishment of an at-line nanofractionation-based screening platform by coupling HPLC-MS/MS with high-throughput fluorescence polarization bioassay for natural SARS-CoV-2 fusion inhibitors

Yuan,

Wang,

Chen

et al. 2024

Journal of Chromatography A

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Application and Development of Targeted Fishing Technology in Natural Product Screening - A Simple Minireview

Su,

Wang,

Wang

et al. 2024

CPA

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Background: The screening of active ingredients in traditional Chinese medicine is an important task in the modernization of traditional Chinese medicine, and the commonly used analytical means in the past were mainly to screen the extracts of traditional Chinese medicine through pharmacological experiments, but the method has major defects. The target fishing strategy provides a new idea for the screening of active ingredients, and it has rapidly become a hot research direction, but there are many methods that need to be summarized and aggregated. Objective: It aims to provide readers with an understanding of the achievements, developments, and dilemmas of target fishing techniques over the past few years and to provide new ideas for subsequent research. Methods: Research articles in recent years using target fishing as an entry point are used as a basis to summarize the types of literature based on their principles and characteristics and to discuss the advantages and disadvantages of each method. Conclusion: This paper summarizes the classification and development of fishing techniques such as ultrafiltration, equilibrium dialysis, cell membrane chromatography, and immobilization of target molecules and target fishing and describes the principles and characteristics of these methods. The applications of these methods in the active ingredients of traditional Chinese medicine are summarized, and the problems and solutions of these methods are discussed.

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High-throughput discovery of highly selective reversible hMAO-B inhibitors based on at-line nanofractionation

Cited by 3 publications

References 63 publications

Decoction derived from Allium ascalonicum L. bulbs and Sojae Semen Praeparatum alleviates wind-cold-type common cold via Nrf2/HO-1 pathway and modulation of Lactobacillus murinus level

Decoction derived from Allium ascalonicum L. bulbs and Sojae Semen Praeparatum alleviates wind-cold-type common cold via Nrf2/HO-1 pathway and modulation of Lactobacillus murinus level

Establishment of an at-line nanofractionation-based screening platform by coupling HPLC-MS/MS with high-throughput fluorescence polarization bioassay for natural SARS-CoV-2 fusion inhibitors

Application and Development of Targeted Fishing Technology in Natural Product Screening - A Simple Minireview

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