High-throughput determination of vancomycin in human plasma by a cost-effective system of two-dimensional liquid chromatography

Sheng, Yanghao; Zhou, Boting

doi:10.1016/j.chroma.2017.02.061

Cited by 25 publications

(22 citation statements)

References 34 publications

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“…It was found that the IC 50 value was 13.74 ng/L, the IC 20 -IC 80 were 3.43-43.62 ng/L, and the IC 10 reached up to 1.07 ng/L according to the established IC-TRFIA for Van. The LOD value was 1.07 ng/L in the presented IC-TRFIA for Van, which is more sensitive (approximately 60-fold) compared with Kong et al (2017) established IC-ELISA for Van with LOD value of 0.06 ng/mL, and approximately 460-fold, 2.0 × 10 5 -fold and 2.5 × 10 5 -fold compare with Liang et al (2015), Sheng and Zhou (2017) and Usman and Hempel (2016) which were established fluorescence "turn-off" biosensor, two-dimensional liquid chromatography and HPLC for Van with LOD value of 0.46 ng/mL, 0.2 and 0.25 μg/mL respectively. In addition, it was slightly better than Liu, Yan, Hua, and Wang (2013), Hu et al (2018), Zhou et al (2015) and Sheng et al (2016) established IC-TRFIAs for thiacloprid, aflatoxin B 1 , chloramphenicol and diniconazole with LOD value of 1.9, 3.55, 8.0 and 21 ng/L, respectively.…”

Section: Ic-trfia Establishment Of Van and Its Analoguesmentioning

confidence: 79%

Establishment of an ultrasensitive indirect competitive time-resolved fluoroimmunoassay for vancomycin determination

Han

Dong

et al. 2019

Food and Agricultural Immunology

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Vancomycin (Van) is a common antibiotic that pollutes food, feed and ecological environment. In the present study, an anti-Van polyclonal antibodies (pAbs) and Eu 3+-labeled goat anti-rabbit IgG monoclonal antibody based indirect competitive timeresolved fluoroimmunoassay (IC-TRFIA) was established for ultrasensitive determination of Van, and its half-maximum inhibition concentrations (IC 50) was 13.74 ng/L and the limit of detection (LOD) was 1.07 ng/L. The IC-TRFIA showed strong crossreactivity (CRs) of 36.2% for norvancomycin, and less than 0.1% for polymyxin B, kanamycin and ampicillin. It was found that the IC-TRFIA for Van spiked in different milk powder samples determination were with good accuracy, stability and repeatability, and its recoveries are 83.0-97.9%, also with its coefficient of variations (CVs) are 3.0-12.9% in intra-assay and inter-assay. The results showed that the established IC-TRFIA was promising for ultrasensitive determination of Van in food samples. Highlights (1) Prepared high-quality Van-KLH/BSA conjugates. (2) Obtained high activity and purify anti-Van polyclonal antibodies. (3) Prepared high-quality Eu 3+-labelled goat anti-rabbit IgG monoclonal antibody. (4) Firstly established an IC-TRFIA for Van based on the anti-Van polyclonal antibodies and Eu 3+-labelled goat anti-rabbit IgG monoclonal antibody. (5) The IC-TRFIA can be used for ultrasensitive determination of Van in milk powder samples.

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Section: Ic-trfia Establishment Of Van and Its Analoguesmentioning

confidence: 79%

Establishment of an ultrasensitive indirect competitive time-resolved fluoroimmunoassay for vancomycin determination

Han

Dong

et al. 2019

Food and Agricultural Immunology

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“…Furthermore, the 2D‐LC system adopted simulated gradient peak compression technology to control peak broadening and to improve peak shape, which offered high sensitivity (Stevenson, Bassanese, Conlan, & Barnett, ). To our knowledge, the 2D‐LC system has been used for detecting vancomycin and monoterpene indole alkaloids in human serum (Li et al, ; Sheng & Zhou, ; Liu et al, ), while no study has been described for the detection of the VPA in plasma using the 2D‐LC method without any derivatization. In this paper, we developed a 2D‐LC method for the determining of the VPA and applied the method to detect the concentrations of VPA in human blood samples.…”

Section: Introductionmentioning

confidence: 99%

A novel and nonderivatization method for the determination of valproic acid in human serum by two‐dimensional liquid chromatography

Liu

Shang

Yao

et al. 2019

Biomedical Chromatography

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A novel and robust two‐dimensional liquid chromatography with ultraviolet detection method (2D‐LC–UV) was developed and validated for high‐throughput determination of the concentrations of valproic acid (VPA) in human plasma. This 2D‐LC system was composed of a first‐dimensional LC column, a second‐dimensional LC column and an intermediate transfer column. The sample was directly injected into the 2D‐LC system after an easy protein precipitation treatment. After online preconcentration and primary separation by the first‐dimensional column, the target was captured by an intermediate column and then transferred to second‐dimensional column for analysis. The system transferred the target through “central cutting” mode whereby the drug peak was not subject to interference from the matrix. The analysis cycle time was completed within 7.0 min. Compared with other methods that have been developed, the analysis time was reduced and the operation was much easier without any derivatization. The calibration curve was linear over the 5.90–188.94 μg/ml range for the VPA concentrations. The intra‐day and inter‐day precisions were <5.6%. The recoveries were in the range from 95.2 to 98.0%. This method appears to be sensitive, precise, rapid and low‐cost for the quantification of VPA in serum sample.

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“…HPLC is a type of chromatographic separation technique that is commonly coupled with UV, [55][56][57] (photo)diode array (DA), [58][59] fluorescence, 60 mass spectrometry (MS) 51,[61][62][63] or electrochemical 64 detector for the identification and quantification of the analyte(s) of interest. Due to its high sensitivity and specificity, HPLC has been considered the gold standard for Van quantification among the different analytical methods.…”

Section: Hplcmentioning

confidence: 99%

“…58 Especially in complicated biological fluids, HPLC proves to be an accurate and reliable tool to detect Van at concentrations below the therapeutic range which are often unattainable by immunoassays. [55][56] Another advantage of HPLC over immunoassays is the ability to isolate and quantify the degraded products of Van. [55][56] Furthermore, a simultaneous detection of Van and other commonly co-prescribed antibiotics has been performed on HPLC systems which improves the efficiency of analysis.…”

Section: Hplcmentioning

confidence: 99%

“…[55][56] Another advantage of HPLC over immunoassays is the ability to isolate and quantify the degraded products of Van. [55][56] Furthermore, a simultaneous detection of Van and other commonly co-prescribed antibiotics has been performed on HPLC systems which improves the efficiency of analysis. 53,63 However, traditional HPLC systems have several drawbacks 50,56,[61][62] that limited its widespread use in routine laboratories and prompt ongoing efforts to improve existing HPLC techniques.…”

Section: Hplcmentioning

confidence: 99%

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Design and development of novel fluorescence-based detection methods for bio-analytes

Ng¹

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I would like to express my gratitude to many people who have given me their help and support in one way or another throughout my four years of research studies. First of all, I would like to thank Nanyang Technological University for the research scholarship and great opportunity to pursue my graduate studies in such a wonderful environment. I would like to thank the Division of Chemistry and Biological Chemistry (CBC) for the excellent research infrastructure and the devoted staff for their kind assistance that has helped my research experience to be a lot smoother. I would like to express my heartfelt thanks to my supervisor, Assoc. Prof. Edwin Kok Lee Yeow. I know that sometimes you find it difficult to communicate your point across to me, but thank you for not giving up and still being so patient to teach and guide me. You taught me how to be more open-minded to consider different questions and not to reject it hastily. You also taught me how to look at the bigger picture whenever I am stuck with the details of my work. Thank you for guiding me through the different projects and the whole process of publishing a paper with your expertise and hard work. I would also like to thank my co-supervisor, Assoc. Prof. Bengang Xing, for your encouragement and advice throughout the four years. I am very grateful to my mentor and colleague, Dr. Xiangyang Wu. You are a very experienced and knowledgeable researcher. Thank you for teaching me many things from my first year of PhD and always guiding me and helping me to solve many problems. You have a lot of ideas and you are persistent to work on those ideas. The way you conduct research taught me how a research life is and should be like. I would like to thank my collaborators, Assoc. Prof. Howe Siang Tan, Mr.

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High-throughput determination of vancomycin in human plasma by a cost-effective system of two-dimensional liquid chromatography

Cited by 25 publications

References 34 publications

Establishment of an ultrasensitive indirect competitive time-resolved fluoroimmunoassay for vancomycin determination

Establishment of an ultrasensitive indirect competitive time-resolved fluoroimmunoassay for vancomycin determination

A novel and nonderivatization method for the determination of valproic acid in human serum by two‐dimensional liquid chromatography

Design and development of novel fluorescence-based detection methods for bio-analytes

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