High Throughput Determination of Infectious Virus Titers by Kinetic Measurement of infection-Induced Changes in Cell Morphology
Dominik Hotter,
Marco Kunzelmann,
Franziska Kiefer
et al.
Abstract:Infectivity assays are the key analytical technology for development of manufacturing processes for virus-based therapeutics. Here, we introduce a novel assay format that utilizes label-free bright field images to determine the kinetics of infection-dependent changes in cell morphology. In particular, cell rounding is directly proportional to the amount of infectious virus applied, enabling rapid determination of viral titers in relation to a standard curve. Our kinetic infectious virus titer (KIT) assay is st… Show more
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