High-throughput determination of fudosteine in human plasma by liquid chromatography–tandem mass spectrometry, following protein precipitation in the 96-well plate format

Development and validation of a sensitive and rapid non-aqueous LC–ESI-MS/MS method for measurement of diosgenin in the plasma of normal and hyperlipidemic rats: A comparative study

“…When analyzing the supernatant from a plasma sample using protein precipitation, salts and endogenous material are present and can cause ion suppression that will lead to higher variation, which is greater than that of liquid-liquid extracts [23]. A simple liquid-liquid extraction method of using the solvent system composed of n-hexane-ethyl acetate (9:1, v/v) was used according to one report [20].…”

Optimization and validation of a high performance liquid chromatography method for rapid determination of sinafloxacin, a novel fluoroquinolone in rat plasma using a fused-core C18-silica column

“…Utilization of 96-well format plates can improve efficiency and robustness of the assay [15,16]. Various protein precipitants, such as 10% perchloric, 0.5% trichloroacetic acid and acetonitrile were tried to achieve good resolution and high recovery of sinafloxacin from spiked biologic matrices.…”

Development and validation of a LC/MS/MS method for quantification of nobiliside A in rat plasma

“…When analyzing the supernatant from a plasma sample using protein precipitation, salts and endogenous material are present and can cause ion suppression or enhancement that will lead to higher variation, which is greater than that of solid-phase and liquid-liquid extracts [10]. As shown in Table 1, the matrix effect value was 84.58-92.17% (n = 5) for nobiliside A and 86.23% (n = 5) for IS.…”