High-throughput cultivation of heterotrophic bacteria during a spring phytoplankton bloom in the North Sea

Alejandre-Colomo, Carlota; Harder, Jens; Fuchs, Bernhard M.; Rosselló‐Móra, Ramon; Amann, Rudolf

doi:10.1016/j.syapm.2020.126066

Cited by 23 publications

(39 citation statements)

References 64 publications

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“…Metaproteomic data is available at PXD019294. The 16S rRNA gene sequences CAL_4_Ha_Ha_2_144 and CAL_4_HaHa_3_166 were previously deposited [ 84 ] under LR722957 and LR722956. Additional six 16S rRNA gene sequences obtained from spring blooms from Helgoland were deposited under the accession numbers LC549856, LC549863, LC549866, LC549883, LC549897, and LC549963 at the DNA Data Bank of Japan (DDBJ).…”

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Verrucomicrobiota are specialist consumers of sulfated methyl pentoses during diatom blooms

et al. 2021

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Marine algae annually sequester petagrams of carbon dioxide into polysaccharides, which are a central metabolic fuel for marine carbon cycling. Diatom microalgae produce sulfated polysaccharides containing methyl pentoses that are challenging to degrade for bacteria compared to other monomers, implicating these sugars as a potential carbon sink. Free-living bacteria occurring in phytoplankton blooms that specialise on consuming microalgal sugars, containing fucose and rhamnose remain unknown. Here, genomic and proteomic data indicate that small, coccoid, free-living Verrucomicrobiota specialise in fucose and rhamnose consumption during spring algal blooms in the North Sea. Verrucomicrobiota cell abundance was coupled with the algae bloom onset and accounted for up to 8% of the bacterioplankton. Glycoside hydrolases, sulfatases, and bacterial microcompartments, critical proteins for the consumption of fucosylated and sulfated polysaccharides, were actively expressed during consecutive spring bloom events. These specialised pathways were assigned to novel and discrete candidate species of the Akkermansiaceae and Puniceicoccaceae families, which we here describe as Candidatus Mariakkermansia forsetii and Candidatus Fucivorax forsetii. Moreover, our results suggest specialised metabolic pathways could determine the fate of complex polysaccharides consumed during algae blooms. Thus the sequestration of phytoplankton organic matter via methyl pentose sugars likely depend on the activity of specialised Verrucomicrobiota populations.

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Verrucomicrobiota are specialist consumers of sulfated methyl pentoses during diatom blooms

et al. 2021

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“…During the last decade, we have isolated thousands of North Sea bacterial strains (Hahnke & Harder, 2013; Hahnke et al ., 2015; Alejandre-Colomo et al ., 2020). Despite these efforts, only two Formosa strains (Unfried et al ., 2018) and one Reinekea strain (Hahnke et al ., 2015; Avci et al ., 2017) had genomes almost identical to bacterioplankton species that were abundant during blooms.…”

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confidence: 99%

Grazers affect the composition of dissolved storage glycans and thereby bacterioplankton composition during a biphasic North Sea spring algae bloom

Sidhu

Kirstein

Meunier

et al. 2022

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Blooms of marine microalgae play a pivotal role in global carbon cycling. Such blooms entail successive blooms of specialized clades of planktonic bacteria that remineralize algal biomass. We investigated the bacterioplankton response to a bloom in the German Bight in spring 2020. Metagenome sequencing at 30 time-points allowed reconstruction of 251 metagenome-assembled genomes (MAGs), 245 representing as yet uncultured species, while corresponding metatranscriptome sequencing highlighted 50 particularly active MAGs. Together with algae, copepod, protist and bacteria diversity and abundance data in combination with physico-chemical data and antibody-based saccharide measurements, we demonstrate (i) how dissolved primary photoassimilated algal and secondary bacterial storage glycans shape the bacterioplankton community composition, and (ii) how grazing on higher trophic levels determines the release of these abundant glycans. We thus elucidate principles governing how bacterioplankton clades respond to algal blooms and collectively remineralize gigatons of carbon annually on a global scale.

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“…Based on this legacy, culture‐dependent bioprospecting is continuously developing to overcome the shortcomings of traditional culturing and provide innovative culturing strategies [67]. Coupling basic biology and ecology with high‐throughput cultivation techniques resulted in increasing the amount of culturable marine microbial species and led to the isolation of new microbial species such as the first representatives of SAR 11 clade [68]. Unlike the traditional isolation strategy, the single‐cell isolation strategy starts with isolating the microorganism of interest and then enriching single cells to enhance their growth [69].…”

Section: Marine Microbial Bioprospectingmentioning

confidence: 99%

Marine microbial bioprospecting: Exploitation of marine biodiversity towards biotechnological applications—a review

et al. 2022

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The increase in the human population causes an increase in the demand for nutritional supplies and energy resources. Thus, the novel, natural, and renewable resources became of great interest. Here comes the optimistic role of bioprospecting as a promising tool to isolate novel and interesting molecules and microorganisms from the marine environment as alternatives to the existing resources. Bioprospecting of marine metabolites and microorganisms with high biotechnological potentials has gained wide interest due to the variability and richness of the marine environment. Indeed, the existence of extreme conditions that increases the adaptability of marine organisms, especially planktons, allow the presence of interesting biological species that are able to produce novel compounds with multiple health benefits and high economical value. This review aims to provide a comprehensive overview of marine microbial bioprospecting as a growing field of interest. It emphasizes functional bioprospecting that facilitates the discovery of interesting metabolites. Marine bioprospecting was also discussed from a legal aspect for the first time, focusing on the shortcomings of international law. We also summarized the challenges facing bioprospecting in the marine environment including economic feasibility issues.

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High-throughput cultivation of heterotrophic bacteria during a spring phytoplankton bloom in the North Sea

Cited by 23 publications

References 64 publications

Verrucomicrobiota are specialist consumers of sulfated methyl pentoses during diatom blooms

Verrucomicrobiota are specialist consumers of sulfated methyl pentoses during diatom blooms

Grazers affect the composition of dissolved storage glycans and thereby bacterioplankton composition during a biphasic North Sea spring algae bloom

Marine microbial bioprospecting: Exploitation of marine biodiversity towards biotechnological applications—a review

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