High-throughput compound evaluation on 3D networks of neurons and glia in a microfluidic platform

Wevers, Nienke R.; Vught, Remko van; Wilschut, Karlijn J.; Nicolas, Arnaud; Chiang, Chiwan; Lanz, Henriëtte L.; Trietsch, Sebastiaan J.; Joore, Jos; Vulto, Paul

doi:10.1038/srep38856

Cited by 120 publications

(90 citation statements)

References 34 publications

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“…Figure 1 shows the OrganoPlate platform, which encompasses 40 microfluidic cell culture structures embedded in a standard 384-well microtiter plate format (Fig. 1a, b) 9,10 . Each microfluidic channel structure is comprised of three lanes that are connected to corresponding wells of a microtiter plate that function as inlets and outlets to access the microfluidic culture.…”

Section: Resultsmentioning

confidence: 99%

Membrane-free culture and real-time barrier integrity assessment of perfused intestinal epithelium tubes

Trietsch

Naumovska

Kurek

et al. 2018

Drug Metabolism and Pharmacokinetics

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Section: Resultsmentioning

confidence: 99%

Membrane-free culture and real-time barrier integrity assessment of perfused intestinal epithelium tubes

Trietsch

Naumovska

Kurek

et al. 2018

Drug Metabolism and Pharmacokinetics

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“…The potential neurotoxic effects of various compounds were studied by assessing the electrophysiological activity of neurons in the network, the extent of neurite outgrowth, and the cell viability in response to drug treatment ( Figure 7C). [70] Takeda et al also developed a novel neural network culture technique to mimic the layered structure of cerebral cortex by controlling the positions of somata and the direction of neurite outgrowth on the basis of the groove guide connected through three compartment chambers ( Figure 7D). [71] This 3D heterogeneous neural component allows the visualization of the network of two different neural connections and was used to assess tau propagation in Alzheimer's disease.…”

Section: Brain On a Chipmentioning

confidence: 99%

3D Miniaturization of Human Organs for Drug Discovery

Park

Wetzel

Dréau

et al. 2017

Adv Healthcare Materials

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“Engineered human organs” hold promises for predicting the effectiveness and accuracy of drug responses while reducing cost, time, and failure rates in clinical trials. Multiorgan human models utilize many aspects of currently available technologies including self‐organized spherical 3D human organoids, microfabricated 3D human organ chips, and 3D bioprinted human organ constructs to mimic key structural and functional properties of human organs. They enable precise control of multicellular activities, extracellular matrix (ECM) compositions, spatial distributions of cells, architectural organizations of ECM, and environmental cues. Thus, engineered human organs can provide the microstructures and biological functions of target organs and advantageously substitute multiscaled drug‐testing platforms including the current in vitro molecular assays, cell platforms, and in vivo models. This review provides an overview of advanced innovative designs based on the three main technologies used for organ construction leading to single and multiorgan systems useable for drug development. Current technological challenges and future perspectives are also discussed.

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“…Attempts have been made to generate thin‐layer Matrigel cultures using microfluidic technologies, microarray chips, polydimethylsiloxane molds, and wax‐printed chromatography paper . While these platforms offer high‐throughput production capabilities, specialized equipment and materials are required, which can limit accessibility of these techniques to the wider scientific community.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of thin‐layer matrigel‐based constructs for three‐dimensional cell culture

Tsai

Frampton

2018

Biotechnology Progress

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Extracellular matrix‐based hydrogels such as Matrigel are easy‐to‐use, commercially available, and offer environments for three‐dimensional (3‐D) cell culture that mimic native tissue. However, manipulating small volumes of these materials to produce thin‐layer 3‐D culture systems suitable for analysis is difficult because of air–liquid‐substrate interfacial tension effects and evaporation. Here, we demonstrate two simple techniques that use standard liquid‐handling tools and nontreated 96‐well plates to produce uniform, thin‐layer constructs for 3‐D culture of cells in Matrigel. The first technique, the floating 3‐D cell culture method, uses phase‐separating polymers to form a barrier between the dispensed Matrigel, air, and cultureware surface to generate consistently thin hydrogels from volumes as low as 5 μL. These unanchored gels provide a useful assay for investigating airway smooth muscle cell contraction and may have future applications in studying asthma pathophysiology. The second technique, the fixed 3‐D cell culture method, provides an anchored gel system for culturing noncontractile cells (e.g., neurons) where 20 μL of Matrigel is dispensed into the bottom of a well filled with culture medium to form a thin gel containing embedded cells. This technique has potential widespread applications as an accessible 3‐D culture platform for high‐throughput production of disease models for evaluation of novel drug therapies. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 35: e2733, 2019

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High-throughput compound evaluation on 3D networks of neurons and glia in a microfluidic platform

Cited by 120 publications

References 34 publications

Membrane-free culture and real-time barrier integrity assessment of perfused intestinal epithelium tubes

Membrane-free culture and real-time barrier integrity assessment of perfused intestinal epithelium tubes

3D Miniaturization of Human Organs for Drug Discovery

Fabrication of thin‐layer matrigel‐based constructs for three‐dimensional cell culture

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