High throughput comparative assessment of biofilm formation of Candida glabrata on polystyrene material

Sadanandan, Bindu; Ashrit, Priya; Nataraj, Lokesh Kyathsandra; Shetty, Kalidas; Jogalekar, Amruta Puroshottam; Vijayalakshmi, Vaniyamparambath; Hemanth, Beena

doi:10.1007/s11814-021-1054-3

Cited by 7 publications

(13 citation statements)

References 45 publications

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“…In the present study growth, cell viability as well as biofilm formation were not affected by the absence of FBS coating in TSB and the TSBM media. Similar results were reported in our previous study with C. glabrata where cell adherence to substratum was not affected by the absence of FBS precoating for biofilm formation in RPMI media 74 . Species as well as strain differences in biofilm formation have been previously reported that leads to the differences in the pathogenicity of the microbial strains 10 .…”

Section: Resultssupporting

confidence: 91%

“…Species as well as strain differences in biofilm formation have been previously reported that leads to the differences in the pathogenicity of the microbial strains 10 . Biofilm quantification using semi‐quantitative assays like CV assay which quantifies biofilm by binding to the matrix and MTT assay which gives a measure of cell viability are well reported and each assay reveals a different aspect of the biofilm 37,74 …”

Section: Resultsmentioning

confidence: 99%

“…Similar results were reported in our previous study with C. glabrata where cell adherence to substratum was not affected by the absence of FBS precoating for biofilm formation in RPMI media. 74 Species as well as strain differences in biofilm formation have been previously reported that leads to the differences in the pathogenicity of the microbial strains. 10 Biofilm quantification using semi- quantitative assays like CV assay which quantifies biofilm by binding to the matrix and MTT assay which gives a measure of cell viability are well reported and each assay reveals a different aspect of the biofilm.…”

Section: Biofilm Quantificationmentioning

confidence: 99%

“…10 Biofilm quantification using semi- quantitative assays like CV assay which quantifies biofilm by binding to the matrix and MTT assay which gives a measure of cell viability are well reported and each assay reveals a different aspect of the biofilm. 37,74 The approach of using more than one semi-quantitative methods is vital for media optimization for growth and biofilm formation which helps to understand the overall complexity and the nature of biofilm depending on the nutrient composition. This study is important as there are no previous studies that have reported the use of OVAT for initial screening for media formulations followed by statistical-based optimization strategy of RSM for in vitro single and dual species biofilm of C. albicans and E. coli on polystyrene material.…”

Section: Biofilm Quantificationmentioning

confidence: 99%

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A microplate‐based Response Surface Methodology model for growth optimization and biofilm formation on polystyrene polymeric material in a Candida albicans and Escherichia coli co‐culture

Ashrit

Sadanandan

Natraj

et al. 2022

Polymers for Advanced Techs

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Biofilm formation is one of the crucial factors for virulence in Candida albicans, Escherichia coli and their polymicrobial biofilm formed on medical prosthetics. In this study, high biofilm forming C. albicans M207 from a case of invasive candidiasis and a standard strain of bacterial culture E. coli ATCC 39936 were used for initial screening and media optimization using One Variable at a time (OVAT) and Response Surface Methodology (RSM) models, respectively, in polystyrene microtiter plates for in vitro biofilm studies. Sucrose and peptone significantly affected biofilm formation when screened by OVAT. Media optimization by Central Composite Design (CCD) showed that pH significantly affected biofilm formation for all the cultures which was further validated at critical parameters. Biofilm was quantified by growth OD measurements, MTT and CV assays in the presence and absence of FBS precoating and compared with standard Trypticase Soy Broth (TSB) media which did not show any significant difference. This high throughput polystyrene microtiter plate assay for preliminary screening of nutritional components using OVAT and media optimization using RSM model is being reported for the first time for biofilm formation by C. albicans and E. coli in a single and dual species environment. This study is essential for functional analysis of in‐vitro growth and biofilm formation by these two pathogenic strains on polymeric hospital equipments and to develop suitable control measures to inhibit biofilm formation by C. albicans and E. coli single species and their co‐culture.

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Section: Resultssupporting

confidence: 91%

Section: Resultsmentioning

confidence: 99%

Section: Biofilm Quantificationmentioning

confidence: 99%

Section: Biofilm Quantificationmentioning

confidence: 99%

See 2 more Smart Citations

A microplate‐based Response Surface Methodology model for growth optimization and biofilm formation on polystyrene polymeric material in a Candida albicans and Escherichia coli co‐culture

Ashrit

Sadanandan

Natraj

et al. 2022

Polymers for Advanced Techs

Self Cite

View full text Add to dashboard Cite

show abstract

“…We have previously reported that the FBS coating of microtiter plates did not affect cell adhesion and biofilm formation in some of the clinical isolates of C. albicans when grown in RPMI-1640 (49), whereas for the other isolates FBS did play a significant role. We have also reported in our previous study with C. glabrata cultures where biofilm formation in the absence of FBS coating did not make any significant difference to the cell adhesion which is most vital during biofilm formation (50,51). FBS has conventionally been used to mimic the in vivo environment (Thein et (56).…”

Section: Germ Tube Testmentioning

confidence: 72%

Aqueous Spice Extracts as Alternative Antimycotics to Control Highly Drug Resistant Extensive Biofilm Forming Clinical Isolates ofCandida albicans

Sadanandan

Vijayalakshmi

Ashrit

et al. 2023

Preprint

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Candida albicans forms biofilm by associating with biotic and abiotic surfaces. Biofilm formation by C. albicans is relevant and significant as the organisms residing within, gain resistance to conventional antimycotics and are therefore difficult to treat. This study targeted the potential of spice-based antimycotics to control C. albicans biofilms. Ten clinical isolates of C. albicans along with a standard culture MTCC-3017 (ATCC-90028) were screened for their biofilm-forming ability. C. albicans M-207 and C. albicans S-470 were identified as high biofilm formers by point inoculation on TSA medium as they formed a lawn within 16 h and exhibited resistance to fluconazole and caspofungin at 25 mcg and 8 mcg respectively. Aqueous and organic spice extracts were screened for their antimycotic activity against C. albicans M-207 and S-470 by agar and disc diffusion and Zone of Inhibition was observed. Minimal Inhibitory Concentration was determined based on growth absorbance and cell viability measurements. The whole aqueous extract of garlic inhibited biofilms of C. albicans M-207, whereas whole aqueous extracts of garlic, clove, and Indian gooseberry were effective in controlling C. albicans S-470 biofilm within 12 h of incubation. The presence of allicin, ellagic acid, and gallic acid as dominant compounds in the aqueous extracts of garlic, clove, and Indian gooseberry respectively was determined by High-Performance Thin Layer Chromatography and Liquid Chromatography-Mass Spectrometry. The morphology of C. albicans biofilm at different growth periods was also determined through bright field microscopy, phase contrast microscopy, and fluorescence microscopy. The results of this study indicated that the alternate approach in controlling high biofilm-forming, multi-drug resistant clinical isolates of C. albicans M-207 and S-470 using whole aqueous extracts of garlic, clove, and Indian gooseberry is a safe, potential, and cost-effective one that can benefit the health care needs with additional effective therapeutics to treat biofilm infections.

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Unraveling the complexities of antifungal susceptibility testing in Candida spp.: Insights from design of experiments

Carvalho,

Bazana,

Ferrão

et al. 2025

Analytical Biochemistry

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High throughput comparative assessment of biofilm formation of Candida glabrata on polystyrene material

Cited by 7 publications

References 45 publications

A microplate‐based Response Surface Methodology model for growth optimization and biofilm formation on polystyrene polymeric material in a Candida albicans and Escherichia coli co‐culture

A microplate‐based Response Surface Methodology model for growth optimization and biofilm formation on polystyrene polymeric material in a Candida albicans and Escherichia coli co‐culture

Aqueous Spice Extracts as Alternative Antimycotics to Control Highly Drug Resistant Extensive Biofilm Forming Clinical Isolates ofCandida albicans

Unraveling the complexities of antifungal susceptibility testing in Candida spp.: Insights from design of experiments

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