High-throughput colorimetric detection of tyrosine kinase inhibitors based on the aggregation of gold nanoparticles

Oishi, Jun; Han, Xiaoming; Kang, Jeong Hun; Asami, Yoji; Mori, Takeshi; Niidome, Takuro; Katayama, Yoshiki

doi:10.1016/j.ab.2007.08.032

Cited by 31 publications

(16 citation statements)

References 23 publications

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“…However, this method is rapid (,30 min), does not require a phosphorylation process with the use of derived ATP cosubstrate, and obviates the modification of nanoparticles for signal amplification. Because the charge of kinase-specific peptide probe (inhibitor) could exactly be tuned by inserting positively or negatively charged amino acid residues and AuNPs in the both positive and negative forms can be easily prepared, 12,53,54,69 it is believed that the present …”

Section: Resultsmentioning

confidence: 99%

“…Usually, there are two categories of the colorimetric sensors based on the AuNPs used: one is based on the cross-linking aggregation of AuNPs functionalized respectively with recognition elements (eg, avidin and antibody) and kinase-specific peptide, 10,11,14,15 and the other is based on the non-cross-linking aggregation of unmodified AuNPs, which is tuned by the phosphorylation-induced net charge change of substrate peptide. 12,53,54 Although the cross-linking strategies are robust and highly specific, they suffer from the complicated functionalization of AuNPs. The unmodified method is simple and does not require modification of analyte-binding molecules onto the surface of AuNPs, but it shows poor anti-interference ability to high concentration of salts and other components in real samples.…”

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confidence: 99%

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Gold nanoparticles-based electrochemical method for the detection of protein kinase with a peptide-like inhibitor as the bioreceptor

Sun¹,

Chang²,

Zhou³

et al. 2017

IJN

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This article presents a general method for the detection of protein kinase with a peptide-like kinase inhibitor as the bioreceptor, and it was done by converting gold nanoparticles (AuNPs)-based colorimetric assay into sensitive electrochemical analysis. In the colorimetric assay, the kinase-specific aptameric peptide triggered the aggregation of AuNPs in solution. However, the specific binding of peptide to the target protein (kinase) inhibited its ability to trigger the assembly of AuNPs. In the electrochemical analysis, peptides immobilized on a gold electrode and presented as solution triggered together the in situ formation of AuNPs-based network architecture on the electrode surface. Nevertheless, the formation of peptide–kinase complex on the electrode surface made the peptide-triggered AuNPs assembly difficult. Electrochemical impedance spectroscopy was used to measure the change in surface property in the binding events. When a ferrocene-labeled peptide (Fc-peptide) was used in this design, the network of AuNPs/Fc-peptide produced a good voltammetric signal. The competitive assay allowed for the detection of protein kinase A with a detection limit of 20 mU/mL. This work should be valuable for designing novel optical or electronic biosensors and likely lead to many detection applications.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Gold nanoparticles-based electrochemical method for the detection of protein kinase with a peptide-like inhibitor as the bioreceptor

Sun¹,

Chang²,

Zhou³

et al. 2017

IJN

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“…The detection limits of GNP-based assays are surprisingly low, suggesting remarkable sensitivity (Table 1). Overall, colorimetric assays are very promising for assessing the effectiveness of enzyme inhibitors as potential drugs [22,23]. Such enzyme inhibitors could be used for therapeutic interventions in pathologies characterized by excessively active enzymes.…”

Section: Challenges In Gnp-based Nanosensor Developmentmentioning

confidence: 99%

Gold-nanoparticle-based biosensors for detection of enzyme activity

Hutter

Maysinger

2013

Trends in Pharmacological Sciences

149

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“…Several methods have been described for the detection of enzyme inhibitors, such as endonuclease [32], tyrosine kinase [33] and acetylcholinesterase [34] inhibitors, which is a central part of the drug-development process. Cleavage enzymes like nucleases and proteases are important therapeutic targets involved in many crucial cellular events.…”

Section: Individual Assaysmentioning

confidence: 99%

High Throughput Bioassays Using Nanoparticles

Gómez‐Hens¹,

Fernández-Romero²,

Aguilar-Caballos³

2010

CCHTS

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An overview of the usefulness of different nanoparticles to improve the features of high throughput separation and individual and multiplexed detection bioassays is presented. Although the development of microarray and microfluidic systems has expanded the capabilities of these high throughput assays, the combined use of NPs and these devices has provided them with new applications in drug discovery, proteomic and genomic studies, and clinical diagnosis. This article reviews the wide application field of magnetic, gold, silver, semiconductor and other nanoparticles in high throughput bioassays. Also, the versatility of the detection systems described shows that NPs are useful alternatives to fluorescent dyes, which are often used in these assays.

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High-throughput colorimetric detection of tyrosine kinase inhibitors based on the aggregation of gold nanoparticles

Cited by 31 publications

References 23 publications

Gold nanoparticles-based electrochemical method for the detection of protein kinase with a peptide-like inhibitor as the bioreceptor

Gold nanoparticles-based electrochemical method for the detection of protein kinase with a peptide-like inhibitor as the bioreceptor

Gold-nanoparticle-based biosensors for detection of enzyme activity

High Throughput Bioassays Using Nanoparticles

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