High throughput chemical screening supports the involvement of Ca<sup>2+</sup>in cyclic nucleotide-gated ion channel-mediated programmed cell death in Arabidopsis

Abdel-Hamid, Huda; Chin, Kimberley; Moeder, Wolfgang; Yoshioka, Keiko

doi:10.4161/psb.6.11.17502

Cited by 16 publications

(9 citation statements)

References 22 publications

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“…Therefore, an obvious strategy to obtain specific Ca 2+ channel inhibitors is to start an unbiased de novo chemical screen for inhibitors directly in plant systems. A suppressor screen of cpr22 (AtCNGC11/12)-induced seedling lethality yielded 13 chemicals that partially restored seedling growth [ 295 ], which includes three putative Ca 2+ channel blockers, dibucaine erythrosine B and diethylstilbestrol. However, it was not tested if these inhibitors affected the Ca 2+ channelling activity of the hyperactive CNGC-based CPR22 channel [ 295 ].…”

Section: Discussionmentioning

confidence: 99%

“…A suppressor screen of cpr22 (AtCNGC11/12)-induced seedling lethality yielded 13 chemicals that partially restored seedling growth [ 295 ], which includes three putative Ca 2+ channel blockers, dibucaine erythrosine B and diethylstilbestrol. However, it was not tested if these inhibitors affected the Ca 2+ channelling activity of the hyperactive CNGC-based CPR22 channel [ 295 ]. Another approach may be to develop variants of L-type VGGC inhibitors that are more selective in plants.…”

Section: Discussionmentioning

confidence: 99%

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Pharmacological Strategies for Manipulating Plant Ca2+ Signalling

Vriese

Costa

Beeckman

et al. 2018

IJMS

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Calcium is one of the most pleiotropic second messengers in all living organisms. However, signalling specificity is encoded via spatio-temporally regulated signatures that act with surgical precision to elicit highly specific cellular responses. How this is brought about remains a big challenge in the plant field, in part due to a lack of specific tools to manipulate/interrogate the plant Ca2+ toolkit. In many cases, researchers resort to tools that were optimized in animal cells. However, the obviously large evolutionary distance between plants and animals implies that there is a good chance observed effects may not be specific to the intended plant target. Here, we provide an overview of pharmacological strategies that are commonly used to activate or inhibit plant Ca2+ signalling. We focus on highlighting modes of action where possible, and warn for potential pitfalls. Together, this review aims at guiding plant researchers through the Ca2+ pharmacology swamp.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Pharmacological Strategies for Manipulating Plant Ca2+ Signalling

Vriese

Costa

Beeckman

et al. 2018

IJMS

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“…By acting as the source of ATP energy and the intracellular calcium pool, mitochondria perform diverse cellular functions in plant growth and development. Mitochondria have been reported to be involved in plant cell death through the regulation of [Ca 2+ ] cyt homeostasis and ROS generation 41 42 . An elevation in ROS and the expression of senescence-associated genes have been found to be closely related to leaf senescence 43 44 .…”

Section: Discussionmentioning

confidence: 99%

CaM/BAG5/Hsc70 signaling complex dynamically regulates leaf senescence

Xing

Chang

et al. 2016

Sci Rep

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Calcium signaling plays an essential role in plant cell physiology, and chaperone-mediated protein folding directly regulates plant programmed cell death. The Arabidopsis thaliana protein AtBAG5 (Bcl-2-associated athanogene 5) is unique in that it contains both a BAG domain capable of binding Hsc70 (Heat shock cognate protein 70) and a characteristic IQ motif that is specific for Ca2+-free CaM (Calmodulin) binding and hence acts as a hub linking calcium signaling and the chaperone system. Here, we determined crystal structures of AtBAG5 alone and in complex with Ca2+-free CaM. Structural and biochemical studies revealed that Ca2+-free CaM and Hsc70 bind AtBAG5 independently, whereas Ca2+-saturated CaM and Hsc70 bind AtBAG5 with negative cooperativity. Further in vivo studies confirmed that AtBAG5 localizes to mitochondria and that its overexpression leads to leaf senescence symptoms including decreased chlorophyll retention and massive ROS production in dark-induced plants. Mutants interfering the CaM/AtBAG5/Hsc70 complex formation leads to different phenotype of leaf senescence. Collectively, we propose that the CaM/AtBAG5/Hsc70 signaling complex plays an important role in regulating plant senescence.

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“…The plant CNGCs are involved in numerous biological functions varying from plant development and stress tolerance ( Kaplan et al, 2007 ) to disease resistance ( Abdel-Hamid et al, 2011 ; Ma, 2011 ). As regulators of plant development and stress tolerance, AtCNGC1 is involved in Ca 2+ uptake ( Ma et al, 2006 ); a P. patens CNGC ( PpCNGCb ) and its Arabidopsis ortholog ( AtCNGC2 ) are essential to thermotolerance ( Finka et al, 2012 ); AtCNGC3 contributes to seed germination ( Gobert et al, 2006 ); AtCNGC6 mediates heat induced Ca 2+ influx and the acquisition of thermotolerance ( Gao et al, 2012 ); AtCNGC10 is associated with plant growth ( Borsics et al, 2007 ); AtCNGC16 and AtCNGC18 play a role in pollen fertility under stress and pollen tip growth respectively ( Frietsch et al, 2007 ; Tunc-Ozdemir et al, 2013 ); while AtCNGC19 and AtCNGC20 are related to salt tolerance ( Kugler et al, 2009 ).…”

Section: Introductionmentioning

confidence: 99%

Cyclic nucleotide gated channel gene family in tomato: genome-wide identification and functional analyses in disease resistance

Saand

Wen

et al. 2015

Front. Plant Sci.

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The cyclic nucleotide gated channel (CNGC) is suggested to be one of the important calcium conducting channels. Nevertheless, genome-wide identification and systemic functional analysis of CNGC gene family in crop plant species have not yet been conducted. In this study, we performed genome-wide identification of CNGC gene family in the economically important crop tomato (Solanum lycopersicum L.) and analyzed function of the group IVb SlCNGC genes in disease resistance. Eighteen CNGC genes were identified in tomato genome, and four CNGC loci that were misannotated at database were corrected by cloning and sequencing. Detailed bioinformatics analyses on gene structure, domain composition and phylogenetic relationship of the SlCNGC gene family were conducted and the group-specific feature was revealed. Comprehensive expression analyses demonstrated that SlCNGC genes were highly, widely but differently responsive to diverse stimuli. Pharmacological assays showed that the putative CNGC activators cGMP and cAMP enhanced resistance against Sclerotinia sclerotiorum. Silencing of group IVb SlCNGC genes significantly enhanced resistance to fungal pathogens Pythium aphanidermatum and S. sclerotiorum, strongly reduced resistance to viral pathogen Tobacco rattle virus, while attenuated PAMP- and DAMP-triggered immunity as shown by obvious decrease of the flg22- and AtPep1-elicited hydrogen peroxide accumulation in SlCNGC-silenced plants. Additionally, silencing of these SlCNGC genes significantly altered expression of a set of Ca2+ signaling genes including SlCaMs, SlCDPKs, and SlCAMTA3. Collectively, our results reveal that group IV SlCNGC genes regulate a wide range of resistance in tomato probably by affecting Ca2+ signaling.

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High throughput chemical screening supports the involvement of Ca²⁺in cyclic nucleotide-gated ion channel-mediated programmed cell death in Arabidopsis

Cited by 16 publications

References 22 publications

Pharmacological Strategies for Manipulating Plant Ca2+ Signalling

Pharmacological Strategies for Manipulating Plant Ca2+ Signalling

CaM/BAG5/Hsc70 signaling complex dynamically regulates leaf senescence

Cyclic nucleotide gated channel gene family in tomato: genome-wide identification and functional analyses in disease resistance

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High throughput chemical screening supports the involvement of Ca2+in cyclic nucleotide-gated ion channel-mediated programmed cell death in Arabidopsis

Cited by 16 publications

References 22 publications

Pharmacological Strategies for Manipulating Plant Ca2+ Signalling

Pharmacological Strategies for Manipulating Plant Ca2+ Signalling

CaM/BAG5/Hsc70 signaling complex dynamically regulates leaf senescence

Cyclic nucleotide gated channel gene family in tomato: genome-wide identification and functional analyses in disease resistance

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High throughput chemical screening supports the involvement of Ca²⁺in cyclic nucleotide-gated ion channel-mediated programmed cell death in Arabidopsis