"High Throughput Chemical Quantification of Pyruvate in Sweat in Response to Modulation by Nutritional Supplementation and Exercise"

Abstract

A 96-well, high throughput, colorimetric chemical detection method based on 2,4-dinitrophenyl hydrazine (DNPH) reaction of the keto-acid to determine the concentration of pyruvate in human sweat samples is presented. This method represents a miniaturization and optimization of the approach developed by Schwimmer and Weston [1] and modified by Sharma and Lee [2] for assessment of pyruvate in food. Parameters were optimized to accommodate small sample sizes and reagent volumes enabling analyte detection in a simple spectrophotometer-96-well-based detection format.