High-Throughput Capillary Liquid Chromatography Using a Droplet Injection and Application to Reaction Screening

Abstract

The cycle time of a standard liquid chromatography (LC) system is the sum of the time for the chromatographic run and the autosampler injection sequence. Although LC separation times in the 1−10 s range have been demonstrated, injection sequences are commonly >15 s, limiting throughput possible with LC separations. Further, such separations are performed on relatively large bore columns requiring flow rates of ≥5 mL/min, thus generating large volumes of mobile phase waste when used for large scale screening and increasing the difficulty in interfacing to mass spectrometry. Here, a droplet injector system was established that replaces the autosampler with a four-port, two-position valve equipped with a 20 nL internal loop interfaced to a syringe pump and a three-axis positioner to withdraw sample droplets from a well plate. In the system, sample and immiscible fluid are pulled alternately from a well plate into a capillary and then through the injection valve. The valve is actuated when sample fills the loop to allow sequential injection of samples at high throughput. Capillary LC columns with 300 μm inner diameter were used to reduce the consumption of mobile phase and sample. The system achieved 96 separations of 20 nL droplet samples containing 3 components in as little as 8.1 min with 5-s cycle time. This system was coupled to a mass spectrometer through an electrospray ionization source for high-throughput chemical reaction screening.

High-throughput label-free opioid receptor binding assays using an automated desorption electrospray ionization mass spectrometry platform

“…Here we demonstrate an alternative label-free and high-throughput approach for screening of OR binding by drug candidates using high-throughput chromatography-free mass spectrometry (MS), an approach that is increasingly utilized for drug discovery applications. 9–11 Our particular system combines custom and commercial software, robotics, and analytical instrumentation, to allow automated assays and data handling. 12 The core technology of the platform is desorption electrospray ionization (DESI), an ambient ionization method that provides rapid and direct MS analysis of complex samples in the open air.…”