High-Throughput Biochemical Phenotyping for Plants

Ménard, Guillaume; Biais, Benoît; Prodhomme, Duyên; Ballias, Patricia; Petit, J.; Just, Daniel; Rothan, Christophe; Gibon, Yves

doi:10.1016/b978-0-12-397922-3.00009-5

Cited by 9 publications

(3 citation statements)

References 92 publications

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“…Currently, several different phenotyping approaches are used to quantify plant defense against piercing-sucking insects such as aphids. These approaches include insect population assays, use of EPG technique to measure feeding behavior, hand-held spectrophotometry (SPAD meter) to measure chlorophyll content in leaves, ELISAs to measure virus transmission, and plant metabolite assays ( McLean and Kinsey, 1964 ; Tjallingii, 1988 ; Deol et al, 1997 ; Girma et al, 1998 ; Walker, 2000 ; Chan et al, 2010 ; Chen et al, 2012 ; Ménard et al, 2013 ). Most basic screening methods for tolerance include approximating insect population size and estimating plant damage by visual estimations to compare to a known susceptible genotype or cultivar.…”

Section: Quantifying Tolerancementioning

confidence: 99%

Plant Tolerance: A Unique Approach to Control Hemipteran Pests

et al. 2016

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Plant tolerance to insect pests has been indicated to be a unique category of resistance, however, very little information is available on the mechanism of tolerance against insect pests. Tolerance is distinctive in terms of the plant’s ability to withstand or recover from herbivore injury through growth and compensatory physiological processes. Because plant tolerance involves plant compensatory characteristics, the plant is able to harbor large numbers of herbivores without interfering with the insect pest’s physiology or behavior. Some studies have observed that tolerant plants can compensate photosynthetically by avoiding feedback inhibition and impaired electron flow through photosystem II that occurs as a result of insect feeding. Similarly, the up-regulation of peroxidases and other oxidative enzymes during insect feeding, in conjunction with elevated levels of phytohormones can play an important role in providing plant tolerance to insect pests. Hemipteran insects comprise some of the most economically important plant pests (e.g., aphids, whiteflies), due to their ability to achieve high population growth and their potential to transmit plant viruses. In this review, results from studies on plant tolerance to hemipterans are summarized, and potential models to understand tolerance are presented.

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Section: Quantifying Tolerancementioning

confidence: 99%

Plant Tolerance: A Unique Approach to Control Hemipteran Pests

et al. 2016

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“…In conclusion this has the potential to help plant breeders assess large numbers of test citrus rootstocks for P. nicotianae tolerance more rapidly through the proposed pipeline. The metabolic markers can be used to predict the selected trait of P. nicotianae tolerance prior to time consuming greenhouse screening by including rootstocks found to constitutively contain these markers (Menard et al 2013). Although the model is specific for P. nicotianae tolerance biomarkers a similar approach may be used to develop models for other Phytophthora species.…”

Section: Resultsmentioning

confidence: 99%

“…These metabolites, in particular from disease free plants are constitutive, and have potential uses as biomarkers for rapid screening of plant genotypes for disease tolerance (Kumaraswamy et al 2011). Biomarkers are organic indicator compounds that can be used as tracers of a given biological trait (Simoneit 2005;Schudoma et al 2012;Menard et al 2013). Metabolic markers are therefore sub-categories of biomarkers and can be either diagnostic, prognostic, or predictive markers (Fernandez et al 2016;Kumar et al 2017).…”

Section: Introductionmentioning

confidence: 99%

Towards developing a metabolic-marker based predictive model for Phytophthora nicotianae tolerance in citrus rootstocks

et al. 2018

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Root rot of citrus trees caused by Phytophthora nicotianae is responsible for severe economic losses in citriculture. Use of resistant rootstocks is an effective method of managing this problem, however, breeding and selection of new citrus rootstocks is a time-consuming undertaking. The objective was to develop a method for the rapid assessment of rootstocks for P. nicotianae tolerance, using a metabolomics approach to identify metabolic markers for the phenotypic trait of tolerance. Sixteen citrus rootstocks were inoculated with P. nicotianae in the greenhouse for determination of relative tolerance/susceptibility. Healthy citrus roots from four tolerant and four susceptible rootstocks were used for metabolite analysis with the objective of identifying potential biomarkers. Organic solvent extractions of the roots were prepared and analysed by mass-spectrometry based liquid chromatography, which produced 367 ion features (retention time and m/z). Orthogonal partial least squares discriminant analysis of peak abundance using MarkerLynx software allowed for the identification of ion features that differentiate tolerant and susceptible rootstocks. Using descriptive and inferential statistics based on the ion features of uninoculated tolerant vs. susceptible rootstocks, applying logistic regression, 14 top markers were identified and two of them (22.03_259.0975 and 22.21_313.1445: retention time (rt) and mass to charge ratio (m/z) were accepted as potential metabolic markers. A model that can potentially predict tolerance in citrus rootstocks with >98% accuracy is presented.

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