High‐Throughput Bacterial Mutagenicity Testing: Vitotox™ Assay

Verschaeve, Luc

doi:10.1002/9781118538203.ch11

Cited by 8 publications

(9 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To our knowledge this is the first report that tests the genotoxicity of LNA probes. Since the VITOTOX ® test is known to correlate very well with the Ames assay, which is essentially a gene mutation assay [ 43 ], we can conclude that the Cy3_HP_LNA/2OMe_PS probe does not induce genotoxicity by aSOS-induction mechanism.…”

Section: Discussionmentioning

confidence: 95%

Fluorescence In Vivo Hybridization (FIVH) for Detection of Helicobacter pylori Infection in a C57BL/6 Mouse Model

et al. 2016

View full text Add to dashboard Cite

IntroductionIn this study, we applied fluorescence in vivo hybridization (FIVH) using locked nucleic acid (LNA) probes targeting the bacterial rRNA gene for in vivo detection of H. pylori infecting the C57BL/6 mouse model. A previously designed Cy3_HP_LNA/2OMe_PS probe, complementary to a sequence of the H. pylori 16S rRNA gene, was used. First, the potential cytotoxicity and genotoxicity of the probe was assessed by commercial assays. Further, the performance of the probe for detecting H. pylori at different pH conditions was tested in vitro, using fluorescence in situ hybridization (FISH). Finally, the efficiency of FIVH to detect H. pylori SS1 strain in C57BL/6 infected mice was evaluated ex vivo in mucus samples, in cryosections and paraffin-embedded sections by epifluorescence and confocal microscopy.ResultsH. pylori SS1 strain infecting C57BL/6 mice was successfully detected by the Cy3_HP_LNA/2OMe_PS probe in the mucus, attached to gastric epithelial cells and colonizing the gastric pits. The specificity of the probe for H. pylori was confirmed by microscopy.ConclusionsIn the future this methodology can be used in combination with a confocal laser endomicroscope for in vivo diagnosis of H. pylori infection using fluorescent LNA probes, which would be helpful to obtain an immediate diagnosis. Our results proved for the first time that FIVH method is applicable inside the body of a higher-order animal.

show abstract

Section: Discussionmentioning

confidence: 95%

Fluorescence In Vivo Hybridization (FIVH) for Detection of Helicobacter pylori Infection in a C57BL/6 Mouse Model

et al. 2016

View full text Add to dashboard Cite

show abstract

“…[ 15 23 24 ] The Vitotox ® test was found very suitable and highly efficient for (high throughput) screening of chemicals to determine their genotoxic potential. [ 16 17 ] It was also shown to be particularly useful in the research or prescreening phase in the pharmaceutical industry. [ 23 25 ] The test is usually more sensitive than the Ames assay as it detects lower concentrations of a genotoxic compound.…”

Section: Resultsmentioning

confidence: 99%

“…A detailed description of the Vitotox ® test is given elsewhere. [ 15 16 17 ] In summary, this test employs two different constructs of Salmonella typhymurium TA 104. One has a luciferase gene under the control of a modified recN promoter, which leads to light production when DNA is damaged (TA 104-recN2-4 strain or Genox strain), whereas the second one contains lux -genes under the control of a constitutive promoter so that the light production is not influenced by genotoxic compounds (pr1 or Cytox strain).…”

Section: Methodsmentioning

confidence: 99%

Evaluation of the genotoxicity and cytotoxicity of semipurified fractions from the Mediterranean brown algae, Dictyopteris membranacea

et al. 2016

Self Cite

View full text Add to dashboard Cite

Dictyopteris membranacea, a species of Mediterranean brown algae, is believed to have potential pharmacological and nutritional applications. However, such potentials only make sense when devoid of any adverse health consequences. The present study should be seen in this context. It aimed at evaluating the genotoxicity and cytoxicity of its organic extract (F0) and semi purified fractions (F4, F5, and F6). Extracts were tested using the bacterial Vitotox® test and micronucleus assay in different concentrations (from 1.25 μg/mL up to 100 μg/mL, depending on the test and the extract). Applied concentrations were based on a preliminary dose-finding test with the neutral red uptake assay. The results show that all extracts were not genotoxic in the presence or absence of a rat metabolic enzyme fraction (S9). This is encouraging and justifies further investigations on the therapeutic and other values of this algae.SUMMARY Dictyopteris membranacea extracts and some of their semi purified fractions have important antibacterial properties.The organic extract (F0) and semi purified fractions (F4, F5, and F6) were not genotoxic according to the bacterial Vitotox test.They were also not genotoxic according to the micronucleus test in human C3A cells.Applied concentrations were based on the in-vitro neutral red uptake (NRU) test.

show abstract

“…This is not surprising as it is established that this bacterial genotoxicity test is very performing when pure single compounds are tested but that the interpretation of results from tests of complex mixtures, as are plant extracts, sometimes are more difficult due to an apparent toxic response and/or irregular dose-response curves. 9,16 Toxicity according to the Vitotox test may also be due to another mechanism as that detected by the NRU assay. The Vitotox test never the less remains an interesting screening test which correlates very well with the Ames assay.…”

Section: Bauhinia Racemosa (Sample 7)mentioning

confidence: 99%

“…Ficus vasta, (3) Parkia africana (P. biglobosa (Jacq.) G. Don), (4) Pistacia lentiscus, (5) Ampelopsis brevipedunculata, (6) Derris robbusta, (7) Bauhinia racemosa, (8) Lagerstroemia tomentosa, (9) Cichorium intybus and (10) Bauhinia alba (syn. of Bauhinia variegata var.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Composition and Potential Genotoxic effects of Important Egyptian Medicinal Plants

Rashed¹,

Anthonissen²,

Cappoen³

et al. 2015

Phcog Commn.

Self Cite

View full text Add to dashboard Cite

Introduction: We performed a phytochemical analysis and investigated the potential genotoxic properties of ten different methanol extracts of plants that are frequently used in Egypt for medicinal purposes. Such studies are important because these plants were never thoroughly investigated with respect to their possible potential sideeffects and risks for humans. Materials and Methods: In this study we used the bacterial Vitotox test and alkaline comet assay in human C3A cells to estimate their genotoxic potential. The Vitotox test is an interesting screening test which correlates very well with the well-known Ames assay but has the advantage of being rapid, sensitive and requiring only small amounts of a test compound. The test was conducted in the presence and absence of a metabolizing S9-enzyme fraction. The comet assay is now a widely used and validated genotoxicity test which can be applied in all DNA-containing cells. In this study it was conducted in human C3A cells which conserved phase I and II biotransformation capabilities. The in vitro NRU assay was used to investigate toxicity and utilized as a dose-finding test. Results: None of the plants have shown genotoxic properties although one of them, Derris rubusta, showed borderline genotoxicity in both tests. This plant also contains alkaloids and coumarins, besides flavonoids, carbohydrates, tannins and triterpenes that were also found in the other plants. Conclusion: So far all 10 investigated medicinal plants appeared not genotoxic but due to borderline effects, Derris robusta deserves further complementary investigations.

show abstract

High‐Throughput Bacterial Mutagenicity Testing: Vitotox™ Assay

Cited by 8 publications

References 39 publications

Fluorescence In Vivo Hybridization (FIVH) for Detection of Helicobacter pylori Infection in a C57BL/6 Mouse Model

Fluorescence In Vivo Hybridization (FIVH) for Detection of Helicobacter pylori Infection in a C57BL/6 Mouse Model

Evaluation of the genotoxicity and cytotoxicity of semipurified fractions from the Mediterranean brown algae, Dictyopteris membranacea

Phytochemical Composition and Potential Genotoxic effects of Important Egyptian Medicinal Plants

Contact Info

Product

Resources

About