High-Throughput Association Mapping in Brassica napus L.: Methods and Applications

Gill, Rafaqat Ali; Helal, Md Mostofa Uddin; Tang, Minghua; Hu, Ming; Tong, Chaobo; S, Liu

doi:10.1007/978-1-0716-3024-2_6

Cited by 3 publications

(3 citation statements)

References 79 publications

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“…Canola, a member of the Brassicaceae family, is an important and extensively grown crop globally. It holds the second position, following soybean, in the cultivation of oilseed crops for human consumption of edible oil and animal feed in the world [ 20 ]. Although widely recognized as a salt-resistant plant, its productivity and yield are lowered under high salinity conditions [ 21 ].…”

Section: Introductionmentioning

confidence: 99%

Alleviating salinity stress in canola (Brassica napus L.) through exogenous application of salicylic acid

Ilyas,

Maqsood,

Shahbaz

et al. 2024

BMC Plant Biol

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Canola, a vital oilseed crop, is grown globally for food and biodiesel. With the enormous demand for growing various crops, the utilization of agriculturally marginal lands is emerging as an attractive alternative, including brackish-saline transitional lands. Salinity is a major abiotic stress limiting growth and productivity of most crops, and causing food insecurity. Salicylic acid (SA), a small-molecule phenolic compound, is an essential plant defense phytohormone that promotes immunity against pathogens. Recently, several studies have reported that SA was able to improve plant resilience to withstand high salinity. For this purpose, a pot experiment was carried out to ameliorate the negative effects of sodium chloride (NaCl) on canola plants through foliar application of SA. Two canola varieties Faisal (V1) and Super (V2) were assessed for their growth performance during exposure to high salinity i.e. 0 mM NaCl (control) and 200 mM NaCl. Three levels of SA (0, 10, and 20 mM) were applied through foliar spray. The experimental design used for this study was completely randomized design (CRD) with three replicates. The salt stress reduced the shoot and root fresh weights up to 50.3% and 47% respectively. In addition, foliar chlorophyll a and b contents decreased up to 61–65%. Meanwhile, SA treatment diminished the negative effects of salinity and enhanced the shoot fresh weight (49.5%), root dry weight (70%), chl. a (36%) and chl. b (67%). Plants treated with SA showed an increased levels of both enzymatic i.e. (superoxide dismutase (27%), peroxidase (16%) and catalase (34%)) and non-enzymatic antioxidants i.e. total soluble protein (20%), total soluble sugar (17%), total phenolic (22%) flavonoids (19%), anthocyanin (23%), and endogenous ascorbic acid (23%). Application of SA also increased the levels of osmolytes i.e. glycine betaine (31%) and total free proline (24%). Salinity increased the concentration of Na+ ions and concomitantly decreased the K+ and Ca2+ absorption in canola plants. Overall, the foliar treatments of SA were quite effective in reducing the negative effects of salinity. By comparing both varieties of canola, it was observed that variety V2 (Super) grew better than variety V1 (Faisal). Interestingly, 20 mM foliar application of SA proved to be effective in ameliorating the negative effects of high salinity in canola plants.

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Section: Introductionmentioning

confidence: 99%

Alleviating salinity stress in canola (Brassica napus L.) through exogenous application of salicylic acid

Ilyas,

Maqsood,

Shahbaz

et al. 2024

BMC Plant Biol

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“…Oilseed rape (Brassica napus L.) is an important and widely cultivated crop throughout the world. It is ranked second (after soybean) among oil seed crops cultivated for edible oil for humans, and seed cake for animal consumption [21]. It is an allopolyploid plant species (AACC, 2n = 38), considered to have originated relatively recently (~6700-12,000 years ago) from a small number of natural hybridization events in Europe between two diploid progenitor species, B. rapa L. (AA, 2n = 20) and B. oleracea L. (CC, 2n = 18) [22][23][24].…”

Section: Introductionmentioning

confidence: 99%

Moderate Salinity Stress Increases the Seedling Biomass in Oilseed Rape (Brassica napus L.)

Chen

Bian

et al. 2023

Plants

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Oilseed rape (Brassica napus L.), an important oil crop of the world, suffers various abiotic stresses including salinity stress during the growth stage. While most of the previous studies paid attention to the adverse effects of high salinity stress on plant growth and development, as well as their underlying physiological and molecular mechanisms, less attention was paid to the effects of moderate or low salinity stress. In this study, we first tested the effects of different concentrations of NaCl solution on the seedling growth performance of two oilseed rape varieties (CH336, a semi-winter type, and Bruttor, a spring type) in pot cultures. We found that moderate salt concentrations (25 and 50 mmol L−1 NaCl) can stimulate seedling growth by a significant increase (10~20%, compared to controls) in both above- and underground biomasses, as estimated at the early flowering stage. We then performed RNA-seq analyses of shoot apical meristems (SAMs) from six-leaf-aged seedlings under control (CK), low (LS, 25 mmol L−1), and high (HS, 180 mmol L−1) salinity treatments in the two varieties. The GO and KEGG enrichment analyses of differentially expressed genes (DEGs) demonstrated that such a stimulating effect on seedling growth by low salinity stress may be caused by a more efficient capacity for photosynthesis as compensation, accompanied by a reduced energy loss for the biosynthesis of secondary metabolites and redirecting of energy to biomass formation. Our study provides a new perspective on the cultivation of oilseed rape in saline regions and new insights into the molecular mechanisms of salt tolerance in Brassica crops. The candidate genes identified in this study can serve as targets for molecular breeding selection and genetic engineering toward enhancing salt tolerance in B. napus.

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“…Ampli ed polymorphic bands detected genetic diversity in F 8 populations of Brassica species.Molecular genetic diversity analysis was carried out using ve neutral SSR markers in which only three primers produced bands (60%) with an average of two alleles per locus. Ra2-E03 produced band size of 200-210bp, Ra2-F11 produced band size of 198-202 and Na12-A02 produced bands with size ranging from 196-210bp. These primers detected heterzogosity in the set of 48 Brassica genotypes by producing polymorphic bands.…”

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confidence: 99%

Molecular Characterization of Brassica Species using SSR Markers

Khan,

Jadoon,

Ahmad

et al. 2023

Preprint

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Genetic diversity and relationship among F8-populations of brassica species: B. napus, B. rapa and B. juncea were carried out on eight parental-lines along with their 38 introgressed populations using SSR markers to cover the diversity present in them as broadly as possible. Diversity analysis based on three neutral markers formed four distinct clusters with 1, 3, 19 and 22 genotypes, respectively. Based on these finding traits specific markers were employed which further formed six distinct clusters of selected plants with 3, 17, 2, 6, 3 and 14 genotypes. The cluster analysis identified genotypes with desirable biochemical contents i.e. plant 1 of Bnj43 had low glucosinolate, plant 3 of Bnr38 had high oleic acid content, plant 5 of Bnr38 had low linolenic acid, plant 1 of Bnr23 had low erucic acid while plant 2 of Bnr25 had high oil content and plant 2 of Bnj13 had high protein content. Moreover, the polymorphism information content (PIC) value of neutral as well as trait specific SSRs ranged from 0.5 to 0.96 and 0.45 to 0.95, respectively with an overall PIC value for all primers of 0.67. Considerable genetic diversity found among the F8-populations and their parents using SSR markers proved SSR analysis to be a useful tool. Moreover, based on the current findings, populations Bnj50F8, Bnr38F8, Bnj43F8, Bnj3F8 and Bnj25F8 are suggested to be used as source populations for transfer of desirable traits in future Brassica improvement programs.

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High-Throughput Association Mapping in Brassica napus L.: Methods and Applications

Cited by 3 publications

References 79 publications

Alleviating salinity stress in canola (Brassica napus L.) through exogenous application of salicylic acid

Alleviating salinity stress in canola (Brassica napus L.) through exogenous application of salicylic acid

Moderate Salinity Stress Increases the Seedling Biomass in Oilseed Rape (Brassica napus L.)

Molecular Characterization of Brassica Species using SSR Markers

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