High-throughput assay of nitric oxide metabolites in human plasma without deproteinization by lab-on-a-chip electrophoresis using a zwitterionic additive

Miyado, Takashi; Wakida, Shin‐ichi; Aizawa, Hitoshi; Shibutani, Yasuhiko; Kanie, Tomohiko; Katayama, M.; Nose, Kazuo; Shimouchi, Akito

doi:10.1016/j.chroma.2008.07.065

Cited by 15 publications

(18 citation statements)

References 30 publications

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“…Although other zones of the spectra such as the infrared spectral region have been used [56], UV-Vis is the default detection method in most commercial instruments. Pertinent examples include Beckman [57], Shimadzu [58], Waters [59], Hitachi [60], Caliper [61], Agilent [62, 63], or Bio-Rad [64], just to name a few. The advantages of UV-Vis detection include versatility (most organic analytes absorb light in this range), speed, and a wide variety of commercial sources.…”

Section: - Instrumentationmentioning

confidence: 99%

Recent developments in instrumentation for capillary electrophoresis and microchip‐capillary electrophoresis

2010

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Over the last years there has been an explosion in the number of developments and applications of capillary electrophoresis (CE) and microchip-CE. In part, this growth has been the direct consequence of recent developments in instrumentation associated with CE. This review, which is focused on contributions published in the last five years, is intended to complement the papers presented in this special issue dedicated to Instrumentation and to provide an overview on the general trend and some of the most remarkable developments published in the areas of high voltage power supplies, detectors, auxiliary components, and compact systems. It also includes few examples of alternative uses of and modifications to traditional CE instruments.

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Section: - Instrumentationmentioning

confidence: 99%

Recent developments in instrumentation for capillary electrophoresis and microchip‐capillary electrophoresis

2010

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“…Miyado et al report the use of a quartz glass microchip-CE device from Shimazdu to separate and detect nitric oxide metabolites in human plasma [17–19]. This high-throughput assay does not require deproteinization, and the zwitterionic surfactant CHES was employed to generate separations in less than 1 minute.…”

Section: Microseparationsmentioning

confidence: 99%

Review: Microfluidic applications in metabolomics and metabolic profiling

Kraly

Holcomb

Guan

et al. 2009

Analytica Chimica Acta

118

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Metabolomics is an emerging area of research focused on measuring small molecules in biological samples. There are a number of different types of metabolomics, ranging from global profiling of all metabolites in a single sample to measurement of a selected group of analytes. Microfluidics and related technologies have been used in this research area with good success. The aim of this review article is to summarize the use of microfluidics in metabolomics. Direct application of microfluidics to the determination of small molecules is covered first. Next, important sample preparation methods developed for microfluidics and applicable to metabolomics are covered. Finally, a summary of metabolomic work as it relates to analysis of cellular events using microfluidics is covered. KeywordsMicrofluidics; metabolomics; metabolic profiling; review I INTRODUCTIONThe goal of this review is to summarize the use of microfluidics as a novel tool set for metabolomics, metabolic profiling, and other metabolite related biological studies. To the knowledge of the authors this is the first such review generated, and thus we will include articles dating back as far as 2000 to give the reader a comprehensive view of the state of the art. While this is the first review of this topic, a number of important reviews on topics ranging from microfluidic and general analytical methods applicable to metabolomics [1][2][3][4][5][6] to metabolomic and metabolic profiling [7][8][9][10][11][12] have been published previously. For specific topics, it is suggested the reader consult these works. These reviews are by no means a comprehensive list of all reviews in this field given that several dozen review articles have been published over the last nine years ranging in scope from very specific to more general.Before discussing specific applications of microfluidics to metabolomics we will first define metabolomics and the various subsets of this discipline. While many people are familiar with genomics and proteomics, the terminology associated with metabolomics is not as clearly defined. For the sake of this review, we define metabolomics as five separate categories: 1) metabolomics, 2) metabonomics, 3) metabolic fingerprinting, 4) metabolic profiling, and 5) targeted metabolic profiling [13]. Metabolomics is the overall study of metabolite expression Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final citable form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. NIH Public Access Author ManuscriptAnal Chim Acta. Author manuscript; available in PMC 2010 October 19. Published in final edited form as:Anal Chim Acta. NIH-PA Author ManuscriptNIH-PA Author Manuscript ...

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“…The favored materials for microchip fabrication (including commercially available chips) encompass various polymers, e.g . PMMA 131, 132, 140, polycarbonate 106, 129, PDMS 135–138 or glass 130, 134, 137, 139, 141. There is a certain indication that PDMS substrate material may take a dominating place in prototyping microchip analytical devices, at least at a research stage.…”

Section: Microchip Electrophoresis: On the Way To Routine Usementioning

confidence: 99%

Inorganic species analysis by CE – An overview for 2007–2008

Timerbaev

2009

Electrophoresis

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This review article represents the sixth in a series of reviews on CE applied to inorganic analysis, appearing in this journal, and focuses on material published in 2007-2008. As a fundamental review, it examines primarily those documents in which the emphasis is on advances in general CE methodology which are traditionally set on the attainment of higher detection sensitivity and greater preconcentration factors both in capillary and microchip separation formats. Following a major research trend of the previous review period (see A.R. Timerbaev, Electrophoresis 2007, 28, 3420-3425), publications focusing on the quantification of different element species continue to rapidly outpace other applications. A range of practicable separation and detection designs tailor-made to species-selective analysis are critically discussed in order to assess their impact on the rate of development and wide acceptance of CE in the field.

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High-throughput assay of nitric oxide metabolites in human plasma without deproteinization by lab-on-a-chip electrophoresis using a zwitterionic additive

Cited by 15 publications

References 30 publications

Recent developments in instrumentation for capillary electrophoresis and microchip‐capillary electrophoresis

Recent developments in instrumentation for capillary electrophoresis and microchip‐capillary electrophoresis

Review: Microfluidic applications in metabolomics and metabolic profiling

Inorganic species analysis by CE – An overview for 2007–2008

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