High-throughput animal tracking in chemobehavioral phenotyping: Current limitations and future perspectives

Henry, Jason; Wlodkowic, Donald

doi:10.1016/j.beproc.2020.104226

Cited by 30 publications

(59 citation statements)

References 46 publications

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“…Results showed the preference of insects towards warmer regions of arena. Similar studies were reported on determining the preference of insects under major individual gradients like temperature ( Flinn et al 1992 , Kim et al 2015 , Henry and Wlodkowic 2020 ), moisture ( Parde et al 1998 , Collins and Conyers 2009 ), and gas concentration ( Navarro et al 1981 , White et al 1993 , Parde et al 2004 ). Directional movement of insects was assessed in one-dimensional (1D) and two-dimensional (2D) grain mass.…”

supporting

confidence: 70%

Movement and Distribution of Cryptolestes ferrugineus (Coleoptera: Laemophloeidae) Adults Under Different Temperature Differences in Different Lengths of Horizontal Grain Columns

Anukiruthika

Jayas

Jian

2022

Journal of Insect Science

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Understanding insect movement and distribution is critical for developing an effective insect pest management protocol. Movement and distribution of adult Cryptolestes ferrugineus (Stephens) in response to nominal temperature differences of 5, 10, 15, and 20°C in 1- and 2-m horizontal columns filled with 12.5% moisture content wheat were studied in 24, 48, and 72 h of movement times. In the wheat columns without temperature differences, adults had a diffusion distribution pattern caused by their random movement. Adults showed bias movement to the warmer grain in wheat columns with temperature differences and preferred the warmer grain regardless of the magnitude of temperature differences in less than 24 h. Their distribution did not have significant differences among different movement times in 1- or 2-m columns. About 40% of adults moved to the warmer wheat located at 0.4 to 0.5 m in the 1 m column but did not move to the warmer wheat located at 0.9 to 1 m in the 2-m column. Therefore, length of grain column influenced detection of warmer grain by insects. Adults under different temperatures had a similar response as that under linear temperature gradients.

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supporting

confidence: 70%

Movement and Distribution of Cryptolestes ferrugineus (Coleoptera: Laemophloeidae) Adults Under Different Temperature Differences in Different Lengths of Horizontal Grain Columns

Anukiruthika

Jayas

Jian

2022

Journal of Insect Science

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“…In parallel, automated visual tracking is fast becoming an indispensable tool for quantifying animal behaviour (reviewed in Henry & Wlodkowic, 2020; Panadeiro et al ., 2021). There is now a wide variety of programs available for tracking animal movement, including a number of recently developed open‐source systems, such as TRex (Fig.…”

Section: Tools and Technologies For Advancing Behavioural Ecotoxicologymentioning

confidence: 99%

Frontiers in quantifying wildlife behavioural responses to chemical pollution

et al. 2022

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Animal behaviour is remarkably sensitive to disruption by chemical pollution, with widespread implications for ecological and evolutionary processes in contaminated wildlife populations. However, conventional approaches applied to study the impacts of chemical pollutants on wildlife behaviour seldom address the complexity of natural environments in which contamination occurs. The aim of this review is to guide the rapidly developing field of behavioural ecotoxicology towards increased environmental realism, ecological complexity, and mechanistic understanding. We identify research areas in ecology that to date have been largely overlooked within behavioural ecotoxicology but which promise to yield valuable insights, including within-and among-individual variation, social networks and collective behaviour, and multi-stressor interactions. Further, we feature methodological and technological innovations that enable the collection of data on pollutant-induced behavioural changes at an unprecedented resolution and scale in the laboratory and the field. In an era of rapid environmental change, there is an urgent need to advance our understanding of the real-world impacts of chemical pollution on wildlife behaviour. This review therefore provides a roadmap of the major outstanding questions in behavioural ecotoxicology and highlights the need for increased cross-talk with other disciplines in order to find the answers.

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“…The latter are used to remove a parallax error (also known as perspective error) caused by an error in magnification when using a wide field lens to view three-dimensional objects that can be at different heights in the optical path. Such technologies are just being developed but promise vastly increased throughputs of data acquisition, as well as the elimination of temporal bias because all samples on one plate can be recorded simultaneously. , See Figure…”

Section: High-throughput Imagingmentioning

confidence: 99%

“…55 In contrast to the above examples, most recent paradigms to achieve ultrahigh-throughput imaging involve elimination of motorized stages in favor of digital cameras with sufficient sensor resolution to enable simultanous imaging of an area covering a large number of test chambers, coupled with ultrawide angle telecentric lenses. 56 The latter are used to remove a parallax error (also known as perspective error) caused by an error in magnification when using a wide field lens to view three-dimensional objects that can be at different heights in the optical path. Such technologies are just being developed but promise vastly increased throughputs of data acquisition, as well as the elimination of temporal bias because all samples on one plate can be recorded simultaneously.…”

Section: ■ High-throughput Imagingmentioning

confidence: 99%

Toward High-Throughput Fish Embryo Toxicity Tests in Aquatic Toxicology

Wlodkowic

Campana

2021

Environ. Sci. Technol.

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Addressing the shift from classical animal testing to high-throughput in vitro and/or simplified in vivo proxy models has been defined as one of the upcoming challenges in aquatic toxicology. In this regard, the fish embryo toxicity test (FET) has gained significant popularity and wide standardization as one of the sensitive alternative approaches to acute fish toxicity tests in chemical risk assessment and water quality evaluation. Nevertheless, despite the growing regulatory acceptance, the actual manipulation, dispensing, and analysis of living fish embryos remains very labor intensive. Moreover, the FET is commonly performed in plastic multiwell plates under static or semistatic conditions, potentially inadequate for toxicity assessment of some organic, easily degradable or highly adsorptive toxicants. Recent technological advances in the field of mechatronics, fluidics and digital vision systems demonstrate promising future opportunities for automation of many analytical stages in embryo toxicity testing. In this review, we highlight emerging advances in fluidic and laboratory automation systems that can prospectively enable high-throughput FET testing (HT-FET) akin to pipelines commonly found in in vitro drug discovery pipelines. We also outline the existing challenges, barriers to future development and provide an outlook of ground-breaking fluidic technologies in embryo toxicity testing.

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High-throughput animal tracking in chemobehavioral phenotyping: Current limitations and future perspectives

Cited by 30 publications

References 46 publications

Movement and Distribution of Cryptolestes ferrugineus (Coleoptera: Laemophloeidae) Adults Under Different Temperature Differences in Different Lengths of Horizontal Grain Columns

Movement and Distribution of Cryptolestes ferrugineus (Coleoptera: Laemophloeidae) Adults Under Different Temperature Differences in Different Lengths of Horizontal Grain Columns

Frontiers in quantifying wildlife behavioural responses to chemical pollution

Toward High-Throughput Fish Embryo Toxicity Tests in Aquatic Toxicology

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