2011
DOI: 10.1016/j.biombioe.2010.11.025
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High throughput Agrobacterium-mediated switchgrass transformation

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Cited by 79 publications
(128 citation statements)
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“…Proline has been used at low concentrations (100-500 mg l −1 ) in combination with casein hydrolysates (Burris et al 2009;Ramamoorthy and Kumar 2012) to induce Type II calli in switchgrass. Li and Qu (2011) used proline alone at high concentration (2 g l −1 ) for friable embryogenic callus induction, and reported that all regenerated plants were albino after calli of cultivar Alamo were subcultured for 14 months. These results suggested that PVCc medium containing casamino acids would be a better medium than prolinecontaining medium to improve shoot regeneration without albino induction.…”
mentioning
confidence: 99%
“…Proline has been used at low concentrations (100-500 mg l −1 ) in combination with casein hydrolysates (Burris et al 2009;Ramamoorthy and Kumar 2012) to induce Type II calli in switchgrass. Li and Qu (2011) used proline alone at high concentration (2 g l −1 ) for friable embryogenic callus induction, and reported that all regenerated plants were albino after calli of cultivar Alamo were subcultured for 14 months. These results suggested that PVCc medium containing casamino acids would be a better medium than prolinecontaining medium to improve shoot regeneration without albino induction.…”
mentioning
confidence: 99%
“…Transgene inheritance and phenotypic expression in progeny derived from Agrobacteriummediated transformation were observed in some of these reports (Li & Qu, 2011;Somleva et al, 2002;Xi et al, 2009). …”
Section: Switchgrass Transformation Systemsmentioning
confidence: 96%
“…For stable transformation of switchgrass, the most noteworthy Agrobacterium strain so far has been EHA105; two different research groups found it to be more effective than other strains, such as AGL1, GV3101, and LBA4404 (Song et al, 2011;Xi et al, 2009). Li & Qu (2011) recently developed the most high-through-put system to date, with a stable transformation efficiency of over 90%. Although most previous transformation systems employed the cultivar 'Alamo', the authors a found higher transformation efficiency with the cultivar 'Performer'.…”
Section: Switchgrass Transformation Systemsmentioning
confidence: 99%
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“…Hygromycin B concentrations in media of more than 50 mg/L have been used in transformation protocols for some monocotyledonous plants (Dong and Qu 2005;Ge et al 2006;Li and Qu 2011). However, nearly all L. rigidum calli showed necrosis within 1 week and almost all calli infected with A. tumefaciens died within 2 weeks of inoculation when subcultured onto media containing 50 mg/L hygromycin B.…”
Section: Establishment Of a Transformation System For Annual Ryegrassmentioning
confidence: 99%