High-temperature wheat leaf rust resistance gene Lr13 exhibits pleiotropic effects on hybrid necrosis

Yan, Xiaohui; Li, Miaomiao; Zhang, Peipei; Guihong, Yin; Zhang, Huaizhi; Gebrewahid, Takele Weldu; Zhang, Jianping; Dong, Lingli; Liu, Daqun; Liu, Zhiyong; Li, Zaifeng

doi:10.1016/j.molp.2021.05.009

Cited by 37 publications

(35 citation statements)

References 10 publications

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“…Cadenza do indeed possess the non-functional variant. Our report is accompanied by a paper (Yan et al, 2021) describing map-based cloning of els1 and LrZH22 that encode the same gene to Lr13/Ne2 m . For MutRenSeq, four independent lr13 mutants of cv.…”

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confidence: 99%

Wheat leaf rust resistance gene Lr13 is a specific Ne2 allele for hybrid necrosis

et al. 2021

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Wheat leaf rust resistance gene Lr13 is a specific Ne2 allele for hybrid necrosis

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“…ETI is activated by pathogen-effector proteins via NLRs [47]. The leaf rust disease resistance genes Lr1, Lr10, Lr13, Lr21, Lr22a, and Lr42 [10,[48][49][50][51][52][53] and the powdery mildew resistance gene Pm21 are NLR genes [17,18]. Two upregulated NLR genes were identified, which had different expression patterns in the addition line II-9-3.…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis Reveals the Gene Expression and Regulatory Characteristics of Broad-Spectrum Immunity to Leaf Rust in a Wheat–Agropyron cristatum 2P Addition Line

Liu

et al. 2022

IJMS

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Wheat leaf rust (caused by Puccinia triticina Erikss.) is among the major diseases of common wheat. The lack of resistance genes to leaf rust has limited the development of wheat cultivars. Wheat–Agropyron cristatum (A. cristatum) 2P addition line II-9-3 has been shown to provide broad-spectrum immunity to leaf rust. To identify the specific A. cristatum resistance genes and related regulatory pathways in II-9-3, we conducted a comparative transcriptome analysis of inoculated and uninoculated leaves of the resistant addition line II-9-3 and the susceptible cultivar Fukuhokomugi (Fukuho). The results showed that there were 66 A. cristatum differentially expressed genes (DEGs) and 1389 wheat DEGs in II-9-3 during P. triticina infection. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment and gene set enrichment analysis (GSEA) revealed that the DEGs of II-9-3 were associated with plant–pathogen interaction, MAPK signaling pathway–plant, plant hormone signal transduction, glutathione metabolism, and phenylpropanoid biosynthesis. Furthermore, many defense-related A. cristatum genes, such as two NLR genes, seven receptor kinase-encoding genes, and four transcription factor-encoding genes, were identified. Our results indicated that the key step of resistance to leaf rust involves, firstly, the gene expression of chromosome 2P upstream of the immune pathway and, secondly, the effect of chromosome 2P on the co-expression of wheat genes in II-9-3. The disease resistance regulatory pathways and related genes in the addition line II-9-3 thus could play a critical role in the effective utilization of innovative resources for leaf rust resistance in wheat breeding.

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“…We discovered that Yr27 is allelic to the recently cloned leaf rust resistance gene Lr13 (refs. 2 , 3 ). NLRs often form complex gene clusters, and it has been found that different members of such allelic series can confer resistance to various fungal pathogens.…”

Section: Mainmentioning

confidence: 99%

“…5a,b ). Lr13 results not only in leaf rust resistance, but also in hybrid necrosis in certain genetic backgrounds 2 , 3 . Two amino acids in the NB-ARC domain (Ile 346 and Lys 562) of Lr13 are critical for leaf rust resistance specificity 2 .…”

Section: Mainmentioning

confidence: 99%

“…We identified the race-specific disease resistance gene Yr27 , which encodes an intracellular immune receptor, to be a major contributor to this resistance. Yr27 is allelic to the leaf rust resistance gene Lr13 ; the Yr27 and Lr13 proteins show 97% sequence identity 2 , 3 . Our results demonstrate the feasibility of generating chromosome-scale wheat assemblies to clone genes, and exemplify that highly similar alleles of a single-copy gene can confer resistance to different pathogens, which might provide a basis for engineering Yr27 alleles with multiple recognition specificities in the future.…”

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Long-read genome sequencing of bread wheat facilitates disease resistance gene cloning

et al. 2022

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The cloning of agronomically important genes from large, complex crop genomes remains challenging. Here we generate a 14.7 gigabase chromosome-scale assembly of the South African bread wheat (Triticum aestivum) cultivar Kariega by combining high-fidelity long reads, optical mapping and chromosome conformation capture. The resulting assembly is an order of magnitude more contiguous than previous wheat assemblies. Kariega shows durable resistance to the devastating fungal stripe rust disease1. We identified the race-specific disease resistance gene Yr27, which encodes an intracellular immune receptor, to be a major contributor to this resistance. Yr27 is allelic to the leaf rust resistance gene Lr13; the Yr27 and Lr13 proteins show 97% sequence identity2,3. Our results demonstrate the feasibility of generating chromosome-scale wheat assemblies to clone genes, and exemplify that highly similar alleles of a single-copy gene can confer resistance to different pathogens, which might provide a basis for engineering Yr27 alleles with multiple recognition specificities in the future.

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High-temperature wheat leaf rust resistance gene Lr13 exhibits pleiotropic effects on hybrid necrosis

Cited by 37 publications

References 10 publications

Wheat leaf rust resistance gene Lr13 is a specific Ne2 allele for hybrid necrosis

Wheat leaf rust resistance gene Lr13 is a specific Ne2 allele for hybrid necrosis

Comparative Transcriptome Analysis Reveals the Gene Expression and Regulatory Characteristics of Broad-Spectrum Immunity to Leaf Rust in a Wheat–Agropyron cristatum 2P Addition Line

Long-read genome sequencing of bread wheat facilitates disease resistance gene cloning

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