High Temperature Stress Causes Transient Change in the Photosynthetic Machinery and Sucrose Metabolism of Sugarcane (Saccharum spp.)

Gomathi, R.; Krishnapriya, V.; Kohila, S.; Vasantha, S.; Suresha, G. S.

doi:10.5958/2394-448x.2021.00001.8

Cited by 3 publications

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“…Sucrose synthase enzymes either hydrolyze or synthesize sucrose in sugarcane plant leaves. The expression of SPS, SS and invertase isozymes declined when temperatures increased to 42°C ( Gomathi et al., 2021 ). Conversely, in thermotolerant cultivars, SPS and SS expression levels were higher ( Kohila and Gomathi, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Expression of sucrose metabolizing enzymes in different sugarcane varieties under progressive heat stress

Mehdi,

Liu,

Riaz

et al. 2023

Front. Plant Sci.

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Studying the thermal stress effect on sucrose-metabolizing enzymes in sugarcane is of great importance for understanding acclimation to thermal stress. In this study, two varieties, S2003-US-633 and SPF-238, were grown at three different temperatures ( ± 2°C): 30°C as a control, 45°C for various episodes of high temperature treatments and recovery conditions at 24, 48 and 72 hours. Data showed that reducing sugar content increased until the grand growth stage but sharply declined at the maturity stage in both cultivars. On the other hand, sucrose is enhanced only at the maturity stage. The expression of all invertase isozymes declined prominently; however, the expression of SPS was high at the maturity stage. Hence, the sucrose accumulation in mature cane was due to increased SPS activity while decreased invertase isozymes (vacuolar, cytoplasmic and cell wall) activities at maturity stage in both cultivars. Heat shock decreased the sucrose metabolizing enzymes, sucrose content and sugar recovery rate in both cultivars. In contrast, heat-shock treatments induced maximum proline, MDA, H2O2 and EC in both cultivars. Notably, this is the first report of diverse invertase isozyme molecular weight proteins, such as those with 67, 134 and 160 kDa, produced under heat stress, suggesting that these enzymes have varied activities at different developmental stages. Overall, S2003-US-633 performs better than the cultivar SPF-238 under heat stress conditions at all development stages, with increased sucrose content, enzyme expression, proline and sugar recovery rate. This work will provide a new avenue regarding sugarcane molecular breeding programs with respect to thermal stress.

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Section: Introductionmentioning

confidence: 99%

Expression of sucrose metabolizing enzymes in different sugarcane varieties under progressive heat stress

Mehdi,

Liu,

Riaz

et al. 2023

Front. Plant Sci.

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Assessment of Mango Post-Harvest Losses along Value Chain in the Gamo Zone, Southern Ethiopia

Tarekegn

Kelem

2022

International Journal of Fruit Science

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The Effect of Glucose and Sucrose on the Photosynthetic Pigments Content in Arabidopsis Thalina Upon Heat Stress

Buzduga

Панчук

2022

Biolohichni systemy

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An increase in the Earth's average temperature has a negative effect on the development, growth and productivity of cultivated plants. One of the main metabolic damages caused by exposure to elevated temperatures is excessive production of reactive oxygen species (ROS). ROS, accumulating in chloroplasts, cause significant damage to the photosynthetic apparatus of plants, destroying the chloroplast membrane and damaging pigments. Carbohydrates play a key role in the regulation of the processes of photosynthesis, respiration, seed germination, flowering and aging of plants. Carbohydrates are chemical signaling and sensitive molecules that receive a specific signal under normal conditions and under the influence of stress factors. The aim of our work was to study the effect of exogenous sucrose and glucose on the content of photosynthetic pigments in A. thaliana plants under the influence of heat stress. Two lines of arabidopsiswere used for research: wild-type plants and cat2cat3 knockout line with lacking isoforms of catalase, the main antioxidant enzyme. The plants were grown under conditions of 16-hour daylight at a constant temperature of + 28º C, air humidity of 70% and illumination of 2.5 kL. Treatment was perfomed in the dark for 2 and 4 hours at temperatures of +37 (moderate stress) and +44°C (severe stress treatment). Contorl Plants were incubated in a potassium-phosphate buffer without the addition of sucrose and glucose at a temperature of +20° C. It was shown that the content of chlorophyll a, b and carotenoids in intact plants of the wild type and the knockout line was the same. Under the influence of heat stress, the content of the studied pigments in the presence of sucrose did not change in both lines. During heat stress in the presence of glucose, changes were noted during the 4-hour stress. It was shown that in the control plants incubated at room temperature in the presence of glucose, the content of chlorophyll a and carotenoids increased in wild type. Upon severe heat stress (+44°С), a decrease in the pigment content was jbserved. The data obtained indicate that in conditions of 4 hours of heat stress, exogenous glucose is involved in the plant cell stress response. No changes in the content of chlorophyll and carotenoids were observed in knockout line cat2cat3.

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High Temperature Stress Causes Transient Change in the Photosynthetic Machinery and Sucrose Metabolism of Sugarcane (Saccharum spp.)

Cited by 3 publications

References 0 publications

Expression of sucrose metabolizing enzymes in different sugarcane varieties under progressive heat stress

Expression of sucrose metabolizing enzymes in different sugarcane varieties under progressive heat stress

Assessment of Mango Post-Harvest Losses along Value Chain in the Gamo Zone, Southern Ethiopia

The Effect of Glucose and Sucrose on the Photosynthetic Pigments Content in Arabidopsis Thalina Upon Heat Stress

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